Human Anti-HIV-1 Env Recombinant Antibody (clone VRC06c) (CAT#: MRO-2805CQ)
This product is a recombinant human anti-HIV-1 monoclonal antibody. VRC06c specifically binds to Env.
Specific Inquiry
ELISA
Figure 1 Binding specificity of VRC06 to gp120 defined by ELISA.
VRC06 binding to YU2 gp120 was sensitive to gp120 mutations in both the CD4bs (D368R) and the CoRbs (I420R).
Li, Y., O'Dell, S., Wilson, R., Wu, X., Schmidt, S. D., Hogerkorp, C. M., ... & Chakrabarti, B. K. (2012). HIV-1 neutralizing antibodies display dual recognition of the primary and coreceptor binding sites and preferential binding to fully cleaved envelopglycoproteins. Journal of virology, 86(20), 11231-11241.
ELISA
Figure 2 VRC06 and VRC06b displayed unique binding properties for Env gp120.
VRC06 and VRC06b preferred recognition of Env of CD4-bound conformation (stabilized core, 2CC) and of trimeric context YU2 gp140-F trimer. The CoRbs MAb 17b was used as control.
Li, Y., O'Dell, S., Wilson, R., Wu, X., Schmidt, S. D., Hogerkorp, C. M., ... & Chakrabarti, B. K. (2012). HIV-1 neutralizing antibodies display dual recognition of the primary and coreceptor binding sites and preferential binding to fully cleaved envelopglycoproteins. Journal of virology, 86(20), 11231-11241.
FC
Figure 3 VRC06 and related MAbs recognized quaternary epitopes on Env functional trimer.
Binding of VRC06 to JR-FL gp160 CTtransfected 293T cell surface by FACS assay, represented by MFI (mean of fluorescence intensity).
Li, Y., O'Dell, S., Wilson, R., Wu, X., Schmidt, S. D., Hogerkorp, C. M., ... & Chakrabarti, B. K. (2012). HIV-1 neutralizing antibodies display dual recognition of the primary and coreceptor binding sites and preferential binding to fully cleaved envelopycoproteins. Journal of virology, 86(20), 11231-11241.
Neu
Figure 4 VRC06 neutralization potency against JR-FL gp160 CT virus was affected by Env mutations leading to an alteration of neutralization sensitivity.
Env mutants 301 and T569A/I675V display enhanced neutralization sensitivity to CD4bs MAb F105 but substantially decreased neutralization sensitivity to VRC06.
Li, Y., O'Dell, S., Wilson, R., Wu, X., Schmidt, S. D., Hogerkorp, C. M., ... & Chakrabarti, B. K. (2012). HIV-1 neutralizing antibodies display dual recognition of the primary and coreceptor binding sites and preferential binding to fully cleaved envelopycoproteins. Journal of virology, 86(20), 11231-11241.
Neu
Figure 5 Neutralization potency of VRC06 against JR-FL gp160 CT virus.
The bar graphs depict the effects of the Env mutations 301 and T569A/I675V on the HIV sensitivity (IC50 values) to VRC06 and other CD4bs ligands.
Li, Y., O'Dell, S., Wilson, R., Wu, X., Schmidt, S. D., Hogerkorp, C. M., ... & Chakrabarti, B. K. (2012). HIV-1 neutralizing antibodies display dual recognition of the primary and coreceptor binding sites and preferential binding to fully cleaved envelopoproteins. Journal of virology, 86(20), 11231-11241.
FC
Figure 6 FACS-based binding curves of MAbs to the cell surface JRFL-cleaved trimers.
The CD4bs-directed bNabs
Chakrabarti, B. K., Feng, Y., Sharma, S. K., McKee, K., Hedestam, G. B. K., LaBranche, C. C., ... & Wyatt, R. T. (2013). Robust neutralizing antibodies elicited by HIV-1 JRFL envelope glycoprotein trimers in nonhuman primates. Journal of virology, 87(24), 13239-13251.
Specifications
- Host Species
- Human
- Type
- Human IgG1
- Specificity
- Recognizes HIV-1 Env
- Species Reactivity
- HIV-1
- Clone
- VRC06c
- Applications
- ELISA, FC, Neut
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The HIV-1 Env antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Flow Cytometry, Neutralization.
Target
- Alternative Names
- ENV; gp160; envelope glycoprotein; Envelope surface glycoprotein gp160; precursor; hypothetical protein; Envelope surface glycoprotein gp120; Envelope transmembrane domain
- Gene ID
- 155971
- UniProt ID
- P04578
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-474-S(P) | Recombinant Anti-HIV-1 Env Antibody scFv Fragment | WB, ELISA, IHC, FuncS | scFv |
| HPAB-2324LY-S(P) | Mouse Anti-HIV-1 Env Recombinant Antibody; scFv Fragment (HPAB-2324LY-S(P)) | Neut, ELISA | Mouse scFv |
| HPAB-2325LY-S(P) | Mouse Anti-HIV-1 Env Recombinant Antibody; scFv Fragment (HPAB-2325LY-S(P)) | Neut, ELISA | Mouse scFv |
| HPAB-2326LY-S(P) | Mouse Anti-HIV-1 Env Recombinant Antibody; scFv Fragment (HPAB-2326LY-S(P)) | Neut, ELISA | Mouse scFv |
| HPAB-2327LY-S(P) | Mouse Anti-HIV-1 Env Recombinant Antibody; scFv Fragment (HPAB-2327LY-S(P)) | Neut, ELISA | Mouse scFv |
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-057 | Recombinant Anti-HIV-1 env VHH Single Domain Antibody | ELISA, IHC, FC, FuncS | Llama VHH |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-137 | Human Anti-HIV-1 Env Recombinant Antibody (clone PGT124) | WB, Neut, FuncS | Human IgG |
| PABL-141 | Human Anti-HIV-1 Env Recombinant Antibody (clone 35O22) | WB, ELISA, Neut, FuncS | Human IgG |
| PABL-143 | Human Anti-HIV-1 Env Recombinant Antibody (clone 9H+3L) | WB, ELISA, IF, FuncS | Human IgG |
| PABL-144 | Human Anti-HIV-1 Env Recombinant Antibody (clone C11) | WB, ELISA, FuncS | Human IgG |
| PABL-145 | Human Anti-HIV-1 Env Recombinant Antibody (PABL-145) | ELISA, Neut, FuncS | Human IgG1, κ |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-137 | Human Anti-HIV-1 Env Recombinant Antibody (clone PGT124); Fab Fragment | WB, Neut, FuncS | Human Fab |
| PFBL-141 | Human Anti-HIV-1 Env Recombinant Antibody (clone 35O22); Fab Fragment | WB, ELISA, Neut, FuncS | Human Fab |
| PFBL-143 | Human Anti-HIV-1 Env Recombinant Antibody (clone 9H+3L); Fab Fragment | WB, ELISA, IF, FuncS | Human Fab |
| PFBL-144 | Human Anti-HIV-1 Env Recombinant Antibody (clone C11); Fab Fragment | WB, ELISA, FuncS | Human Fab |
| PFBL-145 | Human Anti-HIV-1 Env Recombinant Antibody; Fab Fragment (PFBL-145) | ELISA, Neut, FuncS | Human Fab |
MHC Tetramer for Virology
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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