Recombinant Human Anti-LPS Antibody (sc12) (CAT#: FAMAB-0207YC)
Recombinant monoclonal antibodies against the lipopolysaccharide of Ralstonia solanacearum (biovar 2, race 3) are provided. The antibody reacted with all race 3 strains tested, but with only some strains belonging to other races. Furthermore, only a few cross-reactions with saprophytic bacteria. Therefore, this antibody is potentially useful for detection of R. solanacearum race 3.
Specific Inquiry
ELISA
Figure 1 Reaction of purified monoclonal single-chain variable antibody fragments (scFv) (anti-LPS 3, anti-LPS 8, anti-LPS 12, and anti-LPS 13) with lipopolysaccharide (LPS) in enzyme-linked immunosorbent assay (ELISA).
ELISA plates were coated with LPS derived from Ralstonia solanacearum.
Griep, R. A., van Twisk, C., van Beckhoven, J. R., van der Wolf, J. M., & Schots, A. (1998). Development of Specific Recombinant Monoclonal Antibodies Against the Lipopolysaccharide of Ralstonia solanacearum Race 3. Phytopathology, 88(8), 795–803.
IF
Figure 2 Immunofluorescence staining of whole Ralstonia solanacearum bacteria.
A surface epitope is recognized by single-chain variable antibody fragment (scFv) anti-lipopolysaccharide (LPS) 12.
Griep, R. A., van Twisk, C., van Beckhoven, J. R., van der Wolf, J. M., & Schots, A. (1998). Development of Specific Recombinant Monoclonal Antibodies Against the Lipopolysaccharide of Ralstonia solanacearum Race 3. Phytopathology, 88(8), 795–803.
WB
Figure 3 Western blot showing that the epitope that is recognized is located on the LPS of R. solanacearum.
Purified LPS, derived from R. solanacearum (tracks 1 and 3) and R. picketti (tracks 2 and 4) was blotted onto nitrocellulose and stained with silver (Bio-Rad Laboratories) or with scFv anti-LPS 12 (tracks 3 and 4), respectively.
Griep, R. A., van Twisk, C., van Beckhoven, J. R., van der Wolf, J. M., & Schots, A. (1998). Development of Specific Recombinant Monoclonal Antibodies Against the Lipopolysaccharide of Ralstonia solanacearum Race 3. Phytopathology, 88(8), 795–803.
Specifications
- Host Species
- Human
- Derivation
- Phage display library
- Type
- Human IgG
- Specificity
- R. solanacearum race 3 LPS
- Species Reactivity
- R. solanacearum race 3
- Clone
- sc12
- Applications
- ELISA, Immunofluorescence, Western Blot
- Related Disease
- Detection of R. solanacearum race 3
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Target
- Alternative Names
- LPS; Lipopolysaccharides
Related Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Single Domain Antibody Products
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IgM Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABL-060 | Recombinant Anti-V. cholerae O1 LPS VHH Single Domain Antibody | WB, ELISA, IP, FuncS | Llama VHH |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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