Recombinant Human Anti-LPS Antibody (sc12)
CAT#: FAMAB-0207YC
Recombinant monoclonal antibodies against the lipopolysaccharide of Ralstonia solanacearum (biovar 2, race 3) are provided. The antibody reacted with all race 3 strains tested, but with only some strains belonging to other races. Furthermore, only a few cross-reactions with saprophytic bacteria. Therefore, this antibody is potentially useful for detection of R. solanacearum race 3.
Published Data
ELISA
Figure 1 Reaction of purified monoclonal single-chain variable antibody fragments (scFv) (anti-LPS 3, anti-LPS 8, anti-LPS 12, and anti-LPS 13) with lipopolysaccharide (LPS) in enzyme-linked immunosorbent assay (ELISA).
ELISA plates were coated with LPS derived from Ralstonia solanacearum.
Griep, R. A., van Twisk, C., van Beckhoven, J. R., van der Wolf, J. M., & Schots, A. (1998). Development of Specific Recombinant Monoclonal Antibodies Against the Lipopolysaccharide of Ralstonia solanacearum Race 3. Phytopathology, 88(8), 795–803.
IF
Figure 2 Immunofluorescence staining of whole Ralstonia solanacearum bacteria.
A surface epitope is recognized by single-chain variable antibody fragment (scFv) anti-lipopolysaccharide (LPS) 12.
Griep, R. A., van Twisk, C., van Beckhoven, J. R., van der Wolf, J. M., & Schots, A. (1998). Development of Specific Recombinant Monoclonal Antibodies Against the Lipopolysaccharide of Ralstonia solanacearum Race 3. Phytopathology, 88(8), 795–803.
WB
Figure 3 Western blot showing that the epitope that is recognized is located on the LPS of R. solanacearum.
Purified LPS, derived from R. solanacearum (tracks 1 and 3) and R. picketti (tracks 2 and 4) was blotted onto nitrocellulose and stained with silver (Bio-Rad Laboratories) or with scFv anti-LPS 12 (tracks 3 and 4), respectively.
Griep, R. A., van Twisk, C., van Beckhoven, J. R., van der Wolf, J. M., & Schots, A. (1998). Development of Specific Recombinant Monoclonal Antibodies Against the Lipopolysaccharide of Ralstonia solanacearum Race 3. Phytopathology, 88(8), 795–803.
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Specifications
- Host Species
- Human
- Derivation
- Phage display library
- Type
- Human IgG
- Specificity
- R. solanacearum race 3 LPS
- Species Reactivity
- R. solanacearum race 3
- Clone
- sc12
- Applications
- ELISA, Immunofluorescence, Western Blot
- Related Disease
- Detection of R. solanacearum race 3
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- Store at -20°C for long-term storage. Avoid freeze/thaw cycles.
Target
- Alternative Names
- LPS; Lipopolysaccharides
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Related Diseases
Rheumatoid Arthritis
Graft-versus-Host Disease
Related Signaling Pathways
TLR Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABL-060 | Recombinant Anti-V. cholerae O1 LPS VHH Single Domain Antibody | WB, ELISA, IP, FuncS | Llama VHH |
| CAT | Product Name | Application | Type |
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| MHH-630-F(E) | Recombinant Human Anti-LPS Antibody Fab Fragment | ELISA, IP, FuncS | Fab |
| PFBL-027 | Recombinant Mouse Anti-C. trachomatis LPS Antibody Fab Fragment (SAG506-01) | WB, ELISA, FuncS | Fab |
| PFBL-078 | Recombinant Mouse Anti-E. coli LPS Antibody Fab Fragment (WN1 222-5) | WB, ELISA, FuncS | Fab |
| PFBZ-112 | Recombinant Mouse Anti-S. flexneri Lipopolysaccharide Antibody Fab Fragment (SYA/J6) | WB, FuncS | IgG |
| HPAB-0235-FY-F(E) | Recombinant Human Anti-LPS Antibody Fab Fragment (1774) | WB, E | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-078 | Recombinant Mouse Anti-E. coli LPS Antibody (WN1 222-5) | WB, ELISA, FuncS | IgG |
| PABL-265 | Recombinant Mouse Anti-LPS Antibody (S25-2) | WB, IHC, FuncS | IgG |
| PABL-477 | Recombinant Mouse Anti-Ft LPS Antibody (PABL-477) | WB, ELISA, Inhib | IgG |
| FAMAB-0104-CN | Recombinant Human Anti-LPS Antibody (S20) | ELISA, FC, BL | Human IgG2 |
| HPAB-0235-FY | Recombinant Human Anti-LPS Antibody (1774) | WB, E | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBZ-112 | Recombinant Mouse Anti-S. flexneri Lipopolysaccharide Antibody scFv Fragment (SYA/J6) | WB, FuncS | scFv |
| FAMAB-0244-CN-S(P) | Recombinant Mouse Anti-LPS Antibody scFv Fragment (CK3) | WB, ELISA | Mouse scFv |
| FAMAB-0245-CN-S(P) | Recombinant Mouse Anti-LPS Antibody scFv Fragment (BML-5D11) | ELISA | Mouse scFv |
| FAMAB-0246-CN-S(P) | Recombinant Mouse Anti-LPS Antibody scFv Fragment (BML-18F8) | ELISA | Mouse scFv |
| FAMAB-0247-CN-S(P) | Recombinant Mouse Anti-LPS Antibody scFv Fragment (BP1-2E7) | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-J0169-YC | Recombinant Human Anti-LPS Antibody (216-01) | ELISA, FuncS | Human IgM, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY23 | Mouse Anti-LPS (clone WN1 222-5) scFv-Fc Chimera | WB, ELISA | Mouse IgG2a, scFv-Fc |
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