Mouse Anti-MCAM Recombinant Antibody (clone PT0278) (CAT#: VS3-CJ960)
This product is a mouse antibody that recognizes human endogenous levels of human CD146.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2147/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2147/if_selected.jpg
Figure 2 Soft tissue
(Fibroblasts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8848/24112_B_2_7.jpg
(Fibroblasts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8848/24112_B_2_7.jpg
Figure 3 Cerebral cortex
(Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Neuronal cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8848/24112_B_9_5.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear Neuronal cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8848/24112_B_9_5.jpg
Figure 4 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8848/24112_A_8_3.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8848/24112_A_8_3.jpg
Figure 5 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Distal tubules Staining: Medium Intensity: Strong Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8848/24112_A_7_5.jpg
(Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Distal tubules Staining: Medium Intensity: Strong Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8848/24112_A_7_5.jpg
Figure 6 Testis
(Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity:>75% Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Peritubular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: Low Intensity: Weak Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8848/24112_A_4_6.jpg
(Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity:>75% Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Peritubular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: Low Intensity: Weak Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8848/24112_A_4_6.jpg
Figure 7 RNA cell line category: Group enriched (HUVEC TERT2, TIME, WM-115)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000076706-MCAM
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000076706-MCAM
Specifications
- Immunogen
- Synthesized peptide derived from human CD146
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human endogenous levels of human CD146
- Species Reactivity
- Human
- Clone
- PT0278
- Applications
- WB, IHC-P
- Conjugate
- Unconjugated
Product Property
- Purification
- Immunogen affinity chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 0.5% BSA, PBS.
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:500-1:2000), Immunohistochemistry-Parraffin (1:100-1:300).
Target
- Alternative Names
- CD146; MUC18; HEMCAM; METCAM; MelCAM
- Gene ID
- 4162
- UniProt ID
- P43121
- Cellular Localization
- Membrane
- Protein Refseq
- NP_006491.2
- Function
- Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "MCAM"
Select a product category from the dropdown menu below to view related products.
Please select product type
Human Antibody Products
Mouse Antibody Products
Chimeric Antibody Products
Humanized Antibody Products
Rabbit Monoclonal Antibody Products
ScFv Antibody Products
IgG Antibody Products
Fab Antibody Products
Antibody Magnetic Beads
ScFv-Fc Chimera Products
Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-516CL | Anti-Human MCAM Recombinant Antibody (ABX-MA1) | WB, FuncS | Antibody |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for VS3-CJ960. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
Application:
-2.png)
Application: IB, ELISA, FC, FuncS
-YJ-3.jpg)
Application: ELISA
-3.jpg)
Application: Neut
Application: ELISA, PD, Activ, PK, Stim
Application: ELISA, FC, FuncS
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
