Chimeric (mouse/human) Anti-MOG Recombinant Antibody (clone h8-18C5) (CAT#: FAMAB-0006WJ)

Figure 1 Myelin and astroglial reactivity of MS-mAb.

Confocal micrographs of immunofluorescence stainings on human MS brain tissue, CIS1-92 (red), anti-MOG 8-18c5 antibody (green).

von Büdingen, H. C., Harrer, M. D., Kuenzle, S., Meier, M., & Goebels, N. (2008). Clonally expanded plasma cells in the cerebrospinal fluid of MS patients produce myelin‐specific antibodies. European journal of immunology, 38(7), 2014-2023.

Figure 2 mAb derived from cePC do not react with MOG or MBP.

Histograms of flow cytometry experiments with a eukaryotic cell line (Jurkat) transfected to express human MOG on its surface. Note the clear reactivity (shift to the right) of humanized anti-MOG h8-18c5 antibody (open histogram). In comparison, no reactivity of MS-mAb or the isotype control (dark grey histogram) with MOG-expressing Jurkat cells could be detected.

von Büdingen, H. C., Harrer, M. D., Kuenzle, S., Meier, M., & Goebels, N. (2008). Clonally expanded plasma cells in the cerebrospinal fluid of MS patients produce myelin‐specific antibodies. European journal of immunology, 38(7), 2014-2023.

Figure 3 mAb derived from cePC do not react with MOG or MBP.

ELISA experiments demonstrating the lack of reactivity with recombinant MOG and purified human MBP.

von Büdingen, H. C., Harrer, M. D., Kuenzle, S., Meier, M., & Goebels, N. (2008). Clonally expanded plasma cells in the cerebrospinal fluid of MS patients produce myelin‐specific antibodies. European journal of immunology, 38(7), 2014-2023.

Figure 4 Ms and control-derived rigg binding to MOg detected with a cell-based assay.

Representative binding of MS (MS-B1) or germinoma control-derived (GCT-A3) rIgG to Jurkat cells transfected with MOG-GFP (left column) or GFP alone (right column). A humanized monoclonal antibody, h8-18c5, specific for human MOG served as a positive control for the Jurkat–MOG–GFP binding.

Willis, S. N., Stathopoulos, P., Chastre, A., Compton, S. D., Hafler, D. A., & O’Connor, K. C. (2015). Investigating the antigen specificity of multiple sclerosis central nervous system-derived immunoglobulins. Frontiers in immunology, 6, 600.