Anti-Mouse EDN1 Immunohistochemistry Kit
CAT#: VS-0525-XY2155
The EDN1 IHC kit provides all-in-one reagents for detecting endothelin 1, a potent vasoconstrictor involved in cardiovascular regulation. Compatible with paraffin and frozen tissues from various species, this kit supports studies on vascular biology, hypertension, and cancer with clear and consistent results.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, nucleoli & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31976/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human colon
Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells and endothelial cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31976/66918_A_9_3_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human colon
Immunohistochemical staining of human colon shows plasma positivity in blood vessels.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37291/87377_A_8_3_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, nucleoli & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31976/321_D9_7_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 5 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm, nucleoli & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31976/323_D9_1_blue_red_green.jpg
Normal Tissue
Figure 6 Lung
Alveolar cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Macrophages
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37291/87377_A_1_4.jpg
Normal Tissue
Figure 7 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37291/87377_A_8_3.jpg
Normal Tissue
Figure 8 Liver
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37291/87377_A_8_4.jpg
Normal Tissue
Figure 9 Kidney
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37291/87377_A_9_5.jpg
Normal Tissue
Figure 10 Testis
Cells in seminiferous ducts
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37291/87377_A_6_6.jpg
RNA Expression
Figure 11 RNA cell line category: Cell line enhanced (EFO-21, HUVEC TERT2, T-47d, TIME)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000078401-EDN1
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Specifications
- Application
- IHC
- Size
- 50 Tests
- Species Reactivity
- Human, Mouse, Rat, Porcine, Canine, Rabbit, Sheep
- Target
- EDN1
- Primary Antibody
- Mouse Anti-EDN1 Antibody
- Secondary Antibody
- Goat anti-Mouse Antibody, HRP
- Sample Type
- FFPE tissue; Frozen section tissue
- Kit Storage
- All reagents should be kept at 2-8°C. The kit remains stable for up to 6 months after arrival.
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Related Signaling Pathways
TNF Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "EDN1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-818-VHH | Recombinant Anti-Human EDN1 VHH Single Domain Antibody | FA, FC, ICC, ELISA | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-1051 | Mouse Anti-EDN1 Recombinant Antibody (clone YC038); Fab Fragment | ELISA, LMNX | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0658CT | Recombinant Mouse anti-Human EDN1 Monoclonal antibody (2D20) | ELISA | |
| VS7-HM837 | Mouse Anti-EDN1 Recombinant Antibody (clone 4E6E5) | ICC, FCM | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-717CQ | Mouse Anti-EDN1 Recombinant Antibody (clone TR.ET.48.5) | Block, ELISA, FC, ICC, IF, IHC, IHC-Fr, IHC-P, IP, RIA, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2154 | Anti-EDN1 Immunohistochemistry Kit | IHC |
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