Mouse Anti-EDN1 Recombinant Antibody (clone 4E6E5)
CAT#: VS7-HM837
Mouse Anti-EDN1 Recombinant Antibody (clone 4E6E5) is an mouse IgG2a antibody that recognizes the Human, Mouse EDN1 by ICC, FCM.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, nucleoli & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31976/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human colon
Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells and endothelial cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31976/66918_A_9_3_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human colon
Immunohistochemical staining of human colon shows plasma positivity in blood vessels.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37291/87377_A_8_3_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, nucleoli & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31976/321_D9_7_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 5 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm, nucleoli & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/31976/323_D9_1_blue_red_green.jpg
Normal Tissue
Figure 6 Lung
Alveolar cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Macrophages
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37291/87377_A_1_4.jpg
Normal Tissue
Figure 7 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37291/87377_A_8_3.jpg
Normal Tissue
Figure 8 Liver
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37291/87377_A_8_4.jpg
Normal Tissue
Figure 9 Kidney
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37291/87377_A_9_5.jpg
Normal Tissue
Figure 10 Testis
Cells in seminiferous ducts
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37291/87377_A_6_6.jpg
RNA Expression
Figure 11 RNA cell line category: Cell line enhanced (EFO-21, HUVEC TERT2, T-47d, TIME)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000078401-EDN1
❮
❯
Specifications
- Immunogen
- Purified recombinant human EDN1 (AA: 18-212) fragment expressed in E. Coli.
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human, Mouse EDN1
- Species Reactivity
- Human, Mouse
- Clone
- 4E6E5
- Applications
- ICC, FCM
- Conjugate
- None
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity-chromatography
- Format
- Liquid
- Molecular Weight
- 24.4KDa
- Concentration
- Please refer to the tube label.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide.
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice pack
Target
- Alternative Names
- EDN1; endothelin 1; endothelin-1; ET1; preproendothelin-1; PPET1; HDLCQ7;
REVIEWS AND Q&AS
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Related Signaling Pathways
TNF Signaling Pathway
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "EDN1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-818-VHH | Recombinant Anti-Human EDN1 VHH Single Domain Antibody | FA, FC, ICC, ELISA | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-1051 | Mouse Anti-EDN1 Recombinant Antibody (clone YC038); Fab Fragment | ELISA, LMNX | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0658CT | Recombinant Mouse anti-Human EDN1 Monoclonal antibody (2D20) | ELISA |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-717CQ | Mouse Anti-EDN1 Recombinant Antibody (clone TR.ET.48.5) | Block, ELISA, FC, ICC, IF, IHC, IHC-Fr, IHC-P, IP, RIA, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2154 | Anti-EDN1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2155 | Anti-Mouse EDN1 Immunohistochemistry Kit | IHC |
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