Rabbit Anti-MYC Recombinant Antibody (clone R03-1I8) (CAT#: VS3-XY3440)

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human, Mouse and Rat MYC

• Species Reactivity
• Human, Mouse, Rat

• Clone
• R03-1I8

• Applications
• WB, IHC-P, ICC, IF, IP, FC

• Related Disease
• Burkitt Lymphoma; High-Grade B-Cell Lymphoma Double-Hit/Triple-Hit

• Clonality
• Monoclonal

• Aggregation
• < 5%

• Format
• Liquid

• Buffer
• PBS (pH 7.4), 150 mM NaCl and 50% glycerol

• Preservative
• 0.02% sodium azide

• Storage
• Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• WB: 1/500-1/1000
  IHC: 1/50-1/100
  IF: 1/50-1/200
  IP: 1/20
  FC: 1/50-1/100

• Alternative Names
• MRTL; MYCC; c-Myc; bHLHe39

• Gene ID
• 4609

• UniProt ID
• P01106

• Long Name
• MYC proto-oncogene, bHLH transcription factor

• Cellular Localization
• Nucleus

• Post Translation Modifications
• Phosphorylated by PRKDC (PubMed:1597196).
  Phosphorylation at Ser-329 by PIM2 leads to the stabilization of MYC (By similarity).
  Phosphorylation at Ser-62 by CDK2 prevents Ras-induced senescence (PubMed:19966300, PubMed:20713526).
  Phosphorylated at Ser-62 by DYRK2; this primes the protein for subsequent phosphorylation by GSK3B at Thr-58 (PubMed:22307329).
  Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome (PubMed:15103331, PubMed:17558397, PubMed:8386367).
  Dephosphorylation at Ser-62 by protein phosphatase 2A (PPP2CA) promotes its degradation; interaction with PPP2CA is enhanced by AMBRA1 (PubMed:25803737, PubMed:25438055).
  Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome (PubMed:15103331, PubMed:17558397, PubMed:25775507).
  In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation (PubMed:17873522, PubMed:17558397).
  In the nucleolus, however, ubiquitination is not counteracted by USP28 but by USP36, due to the lack of interaction between isoform 3 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus (PubMed:17558397, PubMed:25775507).
  Also polyubiquitinated by the DCX(TRPC4AP) complex (PubMed:20551172, PubMed:29779948).
  Ubiquitinated by TRIM6 in a phosphorylation-independent manner (By similarity).

• Protein Refseq
• NP_002458.2

• Function
• Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3' (PubMed:24940000, PubMed:25956029).
  Activates the transcription of growth-related genes (PubMed:24940000, PubMed:25956029).
  Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis (PubMed:24940000, PubMed:25956029).
  Regulator of somatic reprogramming, controls self-renewal of embryonic stem cells (By similarity).

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

Secondary Antibody

• 2RD-A1473-YJ
• Goat Anti-Rabbit IgG-F(ab')2 Secondary antibody

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