Mouse Anti-NFKB1 Monoclonal Antibody (VS7-0425-WR475) (CAT#: VS7-0425-WR475)
This product is a mouse IgG antibody that binds to human NFKB1. The antibody can be used for WB, IHC-P, IF, ICC, FC, and ELISA assays.
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We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27305/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27305/if_selected.jpg
Figure 2 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4031/11361_A_9_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4031/11361_A_9_8.jpg
Figure 3 Colon
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4031/11361_A_9_3.jpg
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4031/11361_A_9_3.jpg
Figure 4 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000109320-NFKB1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000109320-NFKB1
Specifications
- Immunogen
- Recombinant human NFKB1 fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human NFKB1
- Species Reactivity
- Human
- Molecule Class
- NF-κB Transcription Factor
- Applications
- WB, IHC-P, IF, ICC, FC, ELISA
- Conjugate
- Unconjugated
- Related Disease
- Immunodeficiency, common variable, 12, with autoimmunity (CVID12)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- 1 mg/ml
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry: 1:200 - 1:1000
Immunofluorescence: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Target
- Alternative Names
- KBF1; EBP-1; NF-kB; CVID12; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B1; NF-kappabeta
- Gene ID
- 4790
- UniProt ID
- P19838
- Long Name
- Nuclear Factor Kappa B Subunit 1
- Cellular Localization
- Cytoplasm; Nucleus
- Post Translation Modifications
- Acetylation; Hydroxylation; Isopeptide bond; Lipoprotein; Phosphoprotein; S-nitrosylation; Ubl conjugation
- Protein Refseq
- NP_001158884.1
- Function
- Activator; DNA-binding
Recommended Products
Isotype Control
Secondary Antibody
Related Resources
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TCR Signaling Pathway
TLR Signaling Pathway
TNF Signaling Pathway
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1566z | Mouse Anti-NFKB1 Recombinant Antibody (clone 31H12) | WB, IF, IHC | Mouse IgG1 |
| MOB-1711MZ | Recombinant Mouse Anti-Human NFKB1 Antibody | IHC-P, WB | Mouse antibody |
| MRO-2071-CN | Rabbit Anti-NFKB1 Polyclonal Antibody (MRO-2071-CN) | WB, IF, IHC, FC | Rabbit IgG |
| ZG-0312C | Mouse Anti-NFKB1 Recombinant Antibody (ZG-0312C) | WB | Mouse IgG |
| VS3-CJ754 | Rabbit Anti-NFKB1 Recombinant Antibody (VS3-CJ754) | WB, IHC | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
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