Mouse Anti-NGF Recombinant Antibody (clone alphaD11) (CAT#: FAMAB-1225CQ)

Figure 1 ELISA assay with mNGF.

ELISA assay with mNGF coating (5 µg/ml); serial dilutions of parental mAb αD11, chimeric IgG1 αD11 and IgG1 hum-αD11, Protein A-Sepharose purified from transiently cotransfected CHO cells supernatants.

Covaceuszach, S., Marinelli, S., Krastanova, I., Ugolini, G., Pavone, F., Lamba, D., & Cattaneo, A. (2012). Single cycle structure-based humanization of an anti-nerve growth factor therapeutic antibody. PLoS One, 7(3), e32212.

Figure 2 Hum-αD11 preserves the in vitro binding properties of the parental mAb αD11.

Binding curves of a range of concentrations (0.29-37.5 nM) of hNGF to immobilized parental mAb aD11 (immobilization level 1280.0 RU).

Covaceuszach, S., Marinelli, S., Krastanova, I., Ugolini, G., Pavone, F., Lamba, D., & Cattaneo, A. (2012). Single cycle structure-based humanization of an anti-nerve growth factor therapeutic antibody. PLoS One, 7(3), e32212.

Figure 3 Hum-aD11 retains the biological activity of the parental mAb aD11 in vitro.

TrkA phosphorylation inhibition assay in 3T3 TrkA cells. 3T3 TrkA cells were incubated with the indicated combinations of mNGF, parental mAb aD11, IgG1 humɑD11 (purified by Protein A-Sepharose) and the irrelevant mAb SV5 as a negative control. Cell lysates were separated on a 10% SDS gel and phosphorylated TrkA detected using anti-phospho-Y490 TrkA Ab. The ubiquitous band of tubulin served as gel loading control.

Covaceuszach, S., Marinelli, S., Krastanova, I., Ugolini, G., Pavone, F., Lamba, D., & Cattaneo, A. (2012). Single cycle structure-based humanization of an anti-nerve growth factor therapeutic antibody. PLoS One, 7(3), e32212.