Rabbit Anti-NGF Recombinant Antibody (VS13-YC805)

CAT#: VS13-YC805

The Rabbit Anti-NGF Recombinant Antibody is specific for human, mouse, rat and Zebrafish NGF. NGF encodes a secreted protein with nerve growth activity, forming a complex to regulate neuron growth. Mutations link to neuropathies like HSAN5, with expression imbalances associated with allergic rhinitis. The anti-NGF antibody can be used for Western blot (1/2000), Immunofluorescence of cells (1/50-1/200), Immunofluorescence of tissues (1/50-1/200), Immunohistochemistry-paraffin (1/50-1/100), Immunohistochemistry-frozen (1/100).

Gene Expression
Figure 1 RNA cell line category: Cell line enhanced.

Specifications

  • Immunogen
  • Recombinant human NGF, aa 192-241.
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse, Rat and Zebrafish NGF
  • Species Reactivity
  • Human, Mouse, Rat, Zebrafish
  • Applications
  • WB, IHC-P, IHC-Fr, ICC, IF, ELISA
  • Conjugate
  • NONE
  • Related Disease
  • Bone cancer

Product Property

  • Clonality
  • Monoclonal
  • Purification
  • Affinity Chromatography purified
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
  • Preservative
  • 0.05% Sodium azide
  • Storage
  • Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
  • Shipping
  • Shipping with ice pack.

Applications

  • Application Notes
  • Western blot (WB): 1/2000
    Immunofluorescence (IF-cell): 1/50-1/200
    Immunofluorescence (IF-tissue): 1/50-1/200
    Immunohistochemistry-paraffin (IHC-P): 1/50-1/100
    Immunohistochemistry-frozen (IHC-Fr): 1/100

Target

  • Alternative Names
  • Nerve Growth Factor; Nerve Growth Factor (Beta Polypeptide); Beta-NGF; NGFB; Nerve Growth Factor, Beta Subunit; Beta-Nerve Growth Factor; HSAN5;
  • Long Name
  • Nerve Growth Factor
  • Sequence Similarities
  • Belongs to the NGF-beta family.
  • Cellular Localization
  • Secreted
  • Function
  • Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades to regulate neuronal proliferation, differentiation and survival. The immature NGF precursor (proNGF) functions as a ligand for the heterodimeric receptor formed by SORCS2 and NGFR, and activates cellular signaling cascades that lead to inactivation of RAC1 and/or RAC2, reorganization of the actin cytoskeleton and neuronal growth cone collapse.
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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

MSDS

COA

Certificate of Analysis Lookup

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Protocol & Troubleshooting

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Secondary Antibody

Isotype Control

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