Mouse Anti-NHP LPP Recombinant Antibody (clone 8B3A11)
CAT#: VS-1024-XY308
Designed for the specific detection of LPP, this mouse monoclonal antibody demonstrates reactivity with hamster, human, mouse, and non-human primate samples. The immunogen is a purified recombinant fragment of human LPP, providing exceptional specificity. Suitable for a range of applications including Western blot (WB), immunohistochemistry (IHC), immunofluorescence (IF), and ELISA, this antibody is an indispensable tool for exploring LPP expression and function across various biological contexts.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11133/108_A8_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol & focal adhesion sites.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17342/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human Smooth muscle
High expression. RNA expression: 81.3 nTPM
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11133/28447_B_5_7_val_selected.jpg
Normal Tissue
Figure 4 IHC staining of human pancreas
Low expression. RNA expression: 8.8 nTPM
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11133/28447_A_1_3_val_selected.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17342/39943_B_9_5.jpg
Normal Tissue
Figure 6 Colon
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17342/39943_A_7_3.jpg
Normal Tissue
Figure 7 Kidney
Bowman's capsule
Staining: Medium
Intensity: Moderate
Quantity:>75%
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17342/39943_A_8_5.jpg
Normal Tissue
Figure 8 Testis
Leydig cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17342/39943_A_4_6.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17342/39943_A_7_8.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (Daudi, EFO-21, U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000145012-LPP
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Specifications
- Immunogen
- Purified recombinant fragment of human LPP
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- LPP
- Species Reactivity
- Hamster, Human, Mouse, Non-human primate
- Clone
- 8B3A11
- Applications
- WB, IHC, IF, ELISA
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:1000, IHC: 1:200-1:800, IF: 1:200-1:800, ELISA: 1:8000
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4035z | Mouse Anti-LPP Recombinant Antibody (clone 26F4) | WB, ELISA, ICC, IF, IHC | Mouse IgG1 |
| ZG-024R | Mouse Anti-LPP Recombinant Antibody (ZG-024R) | WB, ELISA, IHC, IF | Mouse IgG |
| VS3-QX696 | Mouse Anti-LPP Recombinant Antibody (clone 6F6-C1-B6) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS13-YC672 | Rabbit Anti-LPP Recombinant Antibody (VS13-YC672) | WB, IHC-P, ICC, IF | Rabbit IgG |
| VS7-0425-WR307 | Mouse Anti-LPP Monoclonal Antibody (VS7-0425-WR307) | WB, IHC-P, IF, ICC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2082 | Hi-Affi™ Recombinant Rabbit Anti-LPP Monoclonal Antibody (DS2082AB) | WB, IHC, ICC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1264 | Anti-LPP Immunohistochemistry Kit | IHC | |
| VS-0525-XY4083 | Anti-Mouse LPP Immunohistochemistry Kit | IHC | |
| VS-0525-XY4082 | Anti-Human LPP Immunohistochemistry Kit | IHC |
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