Mouse Anti-TH Recombinant Antibody (clone 1B8D2)
CAT#: VS7-0425-WR979
This product is a mouse IgG1 antibody that binds to human TH. The antibody 1B8D2 recognizes specific amino acid domain of human TH (AA: 44-208). It can be used for WB, IHC, ICC, and FC assays. It cross reacts with rat.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14010/1905_B8_3_selected.jpg
Normal Tissue
Figure 2 High expression in adrenal gland
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61003/159875_B_6_5_val_selected.jpg
Normal Tissue
Figure 3 Low expression in pancreas
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61003/160099_A_3_3_val_selected.jpg
RNA Expression
Figure 4 RNA cell line category: Group enriched (PC-3, SK-BR-3)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000180176-TH
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Specifications
- Immunogen
- Recombinant human TH (AA: 44-208) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human TH
- Species Reactivity
- Human, Rat
- Clone
- 1B8D2
- Molecule Class
- Tyrosine Hydroxylase
- Applications
- WB, IHC, ICC, FC
- Conjugate
- Unconjugated
- Related Disease
- Segawa syndrome autosomal recessive (ARSEGS)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Immunocytochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- TH; EC 1.14.16; Dystonia 14; Tyrosine 3-Hydroxylase; EC 1.14.16.2; Tyrosine 3-Monooxygenase; DYT5b; TYH; Tyrosine Hydroxylase; DYT14
- Gene ID
- 7054
- UniProt ID
- P07101
- Long Name
- Tyrosine Hydroxylase
- Cellular Localization
- Cell projection; Cytoplasm; Cytoplasmic vesicle; Nucleus; Synapse
- Post Translation Modifications
- Phosphoprotein
- Protein Refseq
- NP_000351.2
- Function
- Monooxygenase; Oxidoreductase
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Related Diseases
Amphetamine Addiction
Alcoholism
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| MHC-CN1269 | PE-H-2Kk/Human TH (FETFEAKI) MHC Tetramer | FCM, IHC | |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| VS-0525-XY7226 | Anti-Rat TH Immunohistochemistry Kit | IHC |
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