Mouse Anti-TNFRSF17 Recombinant Antibody (clone 10A9F7) (CAT#: ZG-0264U)

Figure 1 Mouse Anti-TNFRSF17 Antibody (ZG-0264U) in WB

Positive WB detected in: 1-2 lanes: 293F whole cell lysate transfected with BCMA; 3-4 lane: Recombinant proteins with BCMA. All lanes: TNFRSF17 antibody at 1: 1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 40, 15 KDa
Observed band size: 40, 15 KDa
Exposure time: 10min

Figure 2 Mouse Anti-TNFRSF17 Antibody (ZG-0264U) in IF

Immunofluorescence staining of 293F cells transfected with TNFRSF17 with ZG-0264U at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was R-PE-conjugated Goat Anti-Mouse IgG(H+L).

Figure 3 Mouse Anti-TNFRSF17 Antibody (ZG-0264U) in FC

Overlay histogram showing 293F cells transfected with TNFRSF17 stained with ZG-0264U (red line) at 1:100. The cells were fixed in 70% ethanol at 4°C overnight. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was Alexa Fluor 647 Donkey Anti-Mouse IgG (H+L) at 1/250 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (1µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.