Mouse Anti-VEGFA Recombinant Antibody (clone 6G5B5)

Name: Mouse Anti-VEGFA Recombinant Antibody (clone 6G5B5)
SKU: VS7-0425-WR1111
Rating: 5 (1 reviews)

CAT#: VS7-0425-WR1111

This product is a mouse IgG1 antibody that binds to human VEGFA. The antibody 6G5B5 recognizes specific amino acid domain of human VEGFA (AA: 207-371). It can be used for WB and ICC assays.

Gene Expression

Normal Tissue Figure 1 Cerebral cortex

Neuronal cells
Staining:

Medium

Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_B_7_5.jpg

Normal Tissue Figure 2 Thyroid gland

Glandular cells
Staining:

Medium

Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_B_1_5.jpg

Normal Tissue Figure 3 Colon

Glandular cells
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_A_7_3.jpg

Normal Tissue Figure 4 Liver

Cholangiocytes
Staining:

Medium

Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
Hepatocytes
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_A_8_4.jpg

Normal Tissue Figure 5 Kidney

Cells in glomeruli
Staining:

Medium

Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
Cells in tubules
Staining:

Medium

Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_A_9_5.jpg

Normal Tissue Figure 6 Testis

Leydig cells
Staining:

High

Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_A_5_6.jpg

Normal Tissue Figure 7 Lymph node

Non-germinal center cells
Staining:

Medium

Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/39240/82308_A_7_8.jpg

RNA Expression Figure 8 RNA cell line category: Cell line enhanced (hTERT-RPE1, U-87 MG)

Cell lines ordered by descending RNA expression order.

RNA Expression Figure 8 RNA cell line category: Cell line enhanced (hTERT-RPE1, U-87 MG)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000112715-VEGFA

❮

❯

Specifications

Immunogen
Recombinant human VEGFA (AA: 207-371) fragment expressed in E. Coli.

Host Species
Mouse

Type
Mouse IgG1

Specificity
Human VEGFA

Species Reactivity
Human

Clone
6G5B5

Molecule Class
Growth Factor

Applications
WB, ICC

Conjugate
Unconjugated

Related Disease
Microvascular complications of diabetes 1 (MVCD1)

Product Property

Clonality
Monoclonal

Purity
>95% as determined by SDS-PAGE and SEC-HPLC analysis

Format
Liquid

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
0.05% sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Shipping
Ice packs

Applications

Application Notes
Western Blot: 1:500 - 1:2000
Immunocytochemistry: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000

Target

Alternative Names
vanucizumab;1448221-05-3;RG7221 ;VEGFA;vascular endothelial growth factor A;vascular endothelial growth factor , VEGF;VEGF A;VPF;vascular permeability factor;VEGF;MVCD1;MGC70609;

Gene ID
7422

UniProt ID
P15692

Long Name
Vascular Endothelial Growth Factor A

Cellular Localization
Cytoplasm; Endoplasmic reticulum; Extracellular matrix; Golgi apparatus; Nucleus; Secreted

Post Translation Modifications
Disulfide bond; Glycoprotein

Protein Refseq
NP_001020537.2

Function
Developmental protein; Growth factor; Heparin-binding; Mitogen

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS7-0425-WR1111.pdf

MSDS

MSDS - VS7-0425-WR1111.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Secondary Antibody

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