Anti-EGFR-vc-PAB-MODO ADC

Name: Anti-EGFR-vc-PAB-MODO ADC
SKU: ADC-263CL
Rating: 5 (1 reviews)

CAT#: ADC-263CL

This ADC product is composed of an anti-epidermal growth factor receptor (EGFR) antibody conjugated via vc-PAB linker to MODO(cetuximab-VC-MODO). It has demonstrated a response in Colorectal cancer treatment.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cell junctions.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/144_E9_2_selected.jpg

Subcellular Location and Protein Expression Figure 2 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/143_E9_1_blue_red_green.jpg

Normal Tissue Figure 3 Colon

Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_3.jpg

Normal Tissue Figure 4 Liver

Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_4.jpg

Normal Tissue Figure 5 Kidney

Bowman's capsule
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Collecting ducts
Staining:Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Proximal tubules (cell body)
Staining:Medium
Intensity: Strong
Quantity: <25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_5.jpg

Normal Tissue Figure 6 Testis

Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_6_6.jpg

Normal Tissue Figure 7 Placenta

Cytotrophoblasts
Staining:High
Intensity: Strong
Quantity:>75%
Decidual cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Hofbauer cells
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - cell body
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_1_7.jpg

Normal Tissue Figure 8 Lymph node

Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_8.jpg

RNA Expression Figure 9 RNA cell line category: Cell line enriched (A-431)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000146648-EGFR

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Specifications

Antibody Overview
Human-Mouse Chimeric IgGl Antibody Cetuximab (Merck KGaA)

Antibody Isotype
IgG1

Linker
VC-PAB (valine-citrulline-p-aminobenzoyloxycarbonyl)

Linker Class/Description
Class: Enzymatically cleavable linkers-Peptide linkers
Description: peptide linkers, belonging to Enzymatically cleavable linkers, combine greater systemic stability with rapid enzymatic release of the drug in the target cell. The scission of peptidic bonds relies on lysosomal proteolytic enzymes, which have very low activities in blood due to endogenous inhibitors and the unfavorably high pH value of blood.

Drug
MODO (monomethyldolastatin)

Target

Introduction
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

Alternative Names
EGFR; epidermal growth factor receptor; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2; proto-oncogene c-ErbB-1; cell growth inhibiting protein 40; erb-b2 receptor tyrosine kinase 1; cell proliferation-inducing protein 61; receptor tyrosine-protein kinase erbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog;

Gene ID
1956

UniProt ID
P00533

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Related Diseases

Bladder Cancer

Non-small Cell Lung Cancer

Pancreatic Cancer

Hepatocellular Carcinoma

Cushing Syndrome

Related Signaling Pathways

JAK-STAT Signaling Pathway

Downloadable Resources

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Datasheet

Datasheet - ADC-263CL.pdf

MSDS

MSDS - ADC-263CL.pdf

COA

Certificate of Analysis Lookup

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