Recombinant Rabbit Anti-MCM2 (phospho Ser108) Antibody (clone R01-7G9)
CAT#: VS3-FY907
This product is a recombinant rabbit antibody that recognizes MCM2. This antibody has been reported for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation. The clone R01-7G9 is specific for human, mouse, rat MCM2. The antigen is a synthetic phosphopeptide corresponding to residues surrounding ser108 of human mcm2.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31450/1923_C3_26_cr5cd2b031ba569_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31495/721_D2_2_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31496/727_C2_1_selected.jpg
Normal Tissue
Figure 4 Testis
Pachytene spermatocytes Staining: Low Intensity: Moderate Quantity: <25% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/303/2753_A_4_6.jpg
Normal Tissue
Figure 5 Bone marrow
Hematopoietic cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/303/2753_B_6_4.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/303/2753_A_8_8.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000073111-MCM2
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WB
Figure 1. Recombinant Rabbit Anti-MCM2 (phospho Ser108) Antibody (clone R01-7G9) in WB.
Western Blot analysis of Phospho-MCM2 (Ser108) in K562, 3T3, Hela lysates using Phospho-MCM2 (Ser108) Antibody.
ICC
Figure 2. Recombinant Rabbit Anti-MCM2 (phospho Ser108) Antibody (clone R01-7G9) in ICC.
Immunocytochemical analysis of MCM2 (Phospho-Ser108) (green) in Hela using MCM2 (Phospho-Ser108) antibody and DAPI (blue).
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Specifications
- Immunogen
- A synthetic phosphopeptide corresponding to residues surrounding Ser108 of human MCM2.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat MCM2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R01-7G9
- Applications
- Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 102 kDa; Observed MW: 125 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Minichromosome Maintenance Complex Component 2; Minichromosome Maintenance Protein 2 Homolog; Nuclear Protein BM28; Mitotin; CCNL1; CDCL1; BM28; MCM2 Minichromosome Maintenance Deficient 2, Mitotin (S. Cerevisiae); Minichromosome Maintenance Deficient (S. Cerevisiae) 2 (Mitotin); Minichromosome Maintenance Deficient 2 (Mitotin)
- Gene ID
- 4171
- UniProt ID
- P49736
- Sequence Similarities
- Belongs to the MCM family.
- Cellular Localization
- Chromosome, Nucleus
- Post Translation Modifications
- Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.
- Protein Refseq
- NP_004517.2
- Function
- Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed:32453425, PubMed:34694004, PubMed:34700328, PubMed:35585232).
The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed:32453425).
Required for the entry in S phase and for cell division (PubMed:8175912).
Plays a role in terminally differentiated hair cells development of the cochlea and induces cells apoptosis (PubMed:26196677).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "MCM2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1146CT | Recombinant Mouse anti-Human MCM2 Monoclonal antibody (EML554) | ICC, IF, WB | |
| VS3-QX762 | Mouse Anti-MCM2 Recombinant Antibody (clone 2B5-H4-A9) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-FY908 | Recombinant Rabbit Anti-MCM2 (phospho Ser40) Antibody (clone R03-1B3) | WB, IHC-P, IP | Rabbit IgG |
| VS3-FY909 | Recombinant Rabbit Anti-MCM2 (phospho Ser41) Antibody (clone R07-4C3) | WB, IHC-P, IP | Rabbit IgG |
| VS3-FY910 | Recombinant Rabbit Anti-MCM2 (phospho Ser27) Antibody (clone R08-4D8) | WB, IHC-Fr, IHC-P, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0715MZ | Chicken Anti-MCM2 Polyclonal IgY | ICC, IF, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2190 | Hi-Affi™ Recombinant Rabbit Anti-MCM2 Monoclonal Antibody (DS2190AB) | ICC, IF, IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY318 | Mouse Anti-NHP MCM2 Recombinant Antibody (clone 2B3) | WB, IHC, FC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1319 | Anti-MCM2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4304 | Anti-Mouse MCM2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4303 | Anti-Human MCM2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4305 | Anti-Rat MCM2 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC711 | CytoStream™ Rabbit Anti-MCM2 Recombinant Antibody (VS13-YC711) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
| VS13-YC712 | CytoStream™ Mouse Anti-MCM2 Recombinant Antibody (VS13-YC712) | WB, IHC, IF, IP, FC | Mouse IgG1 |
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