Recombinant Human Anti-EGFR Antibody (Nimotuzumab) (CAT#: PABX-052)

Anti-EGFR antibody is a Humanized antibody of IgG class that binds to an EGFR.

Host: Human

Isotype: IgG

Applications: WB, ELISA, FuncS, IB, FC, SPR, Apop


Specific Inquiry
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  • Published Data
  • Tested Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
IB

Figure 1 Binding of nimotuzumab, cetuximab and their monovalent fragments to human tumor cell lines with different EGFR expression.

(A) Immunoblot for the total EGFR and actin were performed for the indicated tumor cells.

Garrido, G., Tikhomirov, I. A., Rabasa, A., Yang, E., Gracia, E., Iznaga, N., ... & Pérez, R. (2011). Bivalent binding by intermediate affinity of nimotuzumab: a contribution to explain antibody clinical profile. Cancer biology & therapy, 11(4), 373-382.

FC

Figure 2 Binding of nimotuzumab, cetuximab and their monovalent fragments to human tumor cell lines with different EGFR expression.

(B) Tumor cells (grey histograms) were stained with an anti-EGFR antibody (black histograms), followed by incubation with FITC-conjugated rabbit anti-rat IgG-H&L.

Garrido, G., Tikhomirov, I. A., Rabasa, A., Yang, E., Gracia, E., Iznaga, N., ... & Pérez, R. (2011). Bivalent binding by intermediate affinity of nimotuzumab: a contribution to explain antibody clinical profile. Cancer biology & therapy, 11(4), 373-382.

SPR

Figure 3 Examination of cetuximab and nimotuzumab binding kinetics using surface plasmon resonance.

(B) Under bivalent binding conditions (intended to represent binding to cells with high EGFR expression), antibodies behaved similarly and accumulated to the equivalent levels; ligand-FcEGFR chimera (dimer); analyte-100 nM anti-EGFR antibodies. Experiments were performed three times with similar results. Representative data are shown.

Garrido, G., Tikhomirov, I. A., Rabasa, A., Yang, E., Gracia, E., Iznaga, N., ... & Pérez, R. (2011). Bivalent binding by intermediate affinity of nimotuzumab: a contribution to explain antibody clinical profile. Cancer biology & therapy, 11(4), 373-382.

Apop

Figure 4 In vitro effects of nimotuzumab and cetuximab on cells with different EGFR expression levels.

(B) Cells treated with antibodies (70 nM) or their monovalent Fab fragments (140 nM) for 24 h were fixed with ice-cold methanol/acetone and stained with PI (400 μg/ml). The percentage of cells with DNA fragmentation (apoptotic cells) was analyzed by flow cytometry. For both experiments the U1906 cell line was used as a negative control for EGFR expression and the fenritenide was used as positive control in the apoptosis assay (data not shown). One representative experiment out of three is shown in each case.

Garrido, G., Tikhomirov, I. A., Rabasa, A., Yang, E., Gracia, E., Iznaga, N., ... & Pérez, R. (2011). Bivalent binding by intermediate affinity of nimotuzumab: a contribution to explain antibody clinical profile. Cancer biology & therapy, 11(4), 373-382.

ELISA

Figure 5 Competition experiments performed for Nimotuzumab.

A, ELISA plates were coated with 5 μg/mL eEGFR and incubated with different mixtures containing a constant concentration of biotinylated Nimotuzumab Fab fragment and decreasing concentrations of the Cetuximab Fab fragment. OD, optical density.

Talavera, A., Friemann, R., Gómez-Puerta, S., Martinez-Fleites, C., Garrido, G., Rabasa, A., ... & Krengel, U. (2009). Nimotuzumab, an antitumor antibody that targets the epidermal growth factor receptor, blocks ligand binding while permitting the active receptor conformation. Cancer research, 69(14), 5851-5859.


Specifications

  • Immunogen
  • The details of the immunogen for this antibody are not available.
  • Host Species
  • Human
  • Derivation
  • Human
  • Type
  • IgG
  • Specificity
  • Tested positive against native Human EGFR
  • Species Reactivity
  • Human
  • Clone
  • Nimotuzumab
  • Applications
  • WB, ELISA, FuncS, IB, FC, SPR, Apop

Product Property

  • Purity
  • >95% by SDS-PAGE and HPLC analysis
  • Storage
  • Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.

Applications

  • Application Notes
  • The EGFR antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Functional Assay, Immunoblotting, Flow Cytometry, Surface Plasmon Resonance, Apoptosis.

Target

  • Alternative Names
  • EGFR; epidermal growth factor receptor; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2; proto-oncogene c-ErbB-1; cell growth inhibiting protein 40; erb-b2 receptor tyrosine kinase 1; cell proliferation-inducing protein 61; receptor tyrosine-protein kinase erbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; mAb806; LICR 806; ABT-806; ch806

Related Resources

  • Biosimilar Overview
  • Related Diseases
  • Related Signaling Pathways
Please click here for more details about the introduction to mechanism of action, clinical projects and approved drugs of Nimotuzumab.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Single-domain Antibody

CAT Product Name Application Type
NABG-056 Recombinant Anti-Mouse Egfr VHH Single Domain Antibody ELISA, IHC, FC, FuncS Llama VHH
PNBL-016 Recombinant Anti-Human EGFR VHH Single Domain Antibody (7D12) WB, ELISA Llama VHH
PNBL-017 Recombinant Anti-Human EGFR VHH Single Domain Antibody (9G8) FuncS, ELISA, IF Llama VHH
PNBL-018 Recombinant Anti-Human EGFR VHH Single Domain Antibody (EgA1) FuncS, SPR Llama VHH
HPAB-AP881-YC Recombinant Llama Anti-EGFR Single Domain Antibody (VHH122) ELISA, FC, IP, FuncS Llama VHH

Recombinant Antibody

CAT Product Name Application Type
PABL-080 Human Anti-EGFR Recombinant Antibody (PABL-080) ELISA, WB, FuncS Human IgG
HPAB-0010-YC Mouse Anti-EGFR Recombinant Antibody (clone L211C) ELISA, FC Mouse IgG
HPAB-0011-YC Human Anti-EGFR Recombinant Antibody (clone Pep 1) FC Human IgG
HPAB-0012-YC Human Anti-EGFR Recombinant Antibody (clone Pep 2) FC Human IgG
HPAB-0013-YC Human Anti-EGFR Recombinant Antibody (clone Pep 3) FC Human IgG

Chimeric Antibody

CAT Product Name Application Type
TAB-0225CL-S(P) Human Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-0225CL-S(P)) Block, Inhib, FuncS, Apop, In vivo Human scFv
TAB-272MZ Human Anti-EGFR Recombinant Antibody (TAB-272MZ) FuncS Chimeric (mouse/human) IgG
TAB-302MZ Human Anti-EGFR Recombinant Antibody (TAB-302MZ) ELISA Chimeric (mouse/human) IgG1
TAB-308MZ Human Anti-EGFR Recombinant Antibody (TAB-308MZ) FuncS Chimeric antibody (mouse/human)
TAB-308MZ-S(P) Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-308MZ-S(P)) ELISA, FC Mouse scFv

Humanized Antibody

CAT Product Name Application Type
TAB-274MZ Human Anti-EGFR Recombinant Antibody (TAB-274MZ) FC Humanized IgG
TAB-274MZ-S(P) Human Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-274MZ-S(P)) FC Humanized scFv
TAB-274MZ-F(E) Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-274MZ-F(E)) FC Humanized Fab
TAB-297MZ-F(E) Anti-Human EGFR Recombinant Antibody Fab Fragment (H225) ELISA, WB Humanized antibody
TAB-298MZ-F(E) Anti-Human EGFR Recombinant Antibody Fab Fragment (Hu225) ELISA, WB Humanized antibody

Human Antibody

CAT Product Name Application Type
TAB-299MZ-S(P) Human Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-299MZ-S(P)) Internalization Assay Human scFv
TAB-270MZ-F(E) Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-270MZ-F(E)) ELISA Human Fab
PABX-052-S (P) Recombinant Human Anti-EGFR Antibody scFv Fragment (Nimotuzumab) WB, ELISA, FuncS scFv
PABX-053-S (P) Recombinant Human Anti-EGFR Antibody scFv Fragment (DL11) WB, ELISA, FuncS scFv
PABX-053-F (E) Recombinant Human Anti-EGFR Antibody Fab Fragment (DL11) WB, ELISA, FuncS Fab

Mouse Antibody

CAT Product Name Application Type
TAB-310MZ-F(E) Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-310MZ-F(E)) ELISA Mouse Fab
TAB-311MZ-F(E) Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-311MZ-F(E)) ELISA Mouse Fab
TAB-312MZ-F(E) Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-312MZ-F(E)) ELISA Mouse Fab
TAB-316MZ-F(E) Anti-Human EGFR Recombinant Antibody Fab Fragment (07D06) WB, MTT assay
TAB-317MZ-F(E) Anti-Human EGFR Recombinant Antibody Fab Fragment (12D03) WB, MTT assay

Fc Glycosylation

CAT Product Name Application Type
Gly-055LC Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated) ELISA Chimeric antibody (mouse/human)
Gly-144LC Recombinant Anti-Human EGFR Antibody (Fc glycosylation) ELISA Humanized antibody

High-mannose Glycoform

CAT Product Name Application Type
Gly-055LC-1 Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated) ELISA Chimeric antibody (mouse/human)

Deglycosylated Antibody (Non-glycosylated IgGs)

CAT Product Name Application Type
Gly-167LC Recombinant Anti-Human EGFR Antibody (Non-glycosylated) ELISA Human antibody

Chicken IgY Antibody

CAT Product Name Application Type
BRD-0183MZ Chicken Anti-EGFR Polyclonal IgY WB Chicken antibody
BRD-0668MZ Chicken Anti-EGFR Polyclonal IgY WB Chicken antibody

MHC Tetramer for Cancer

CAT Product Name Application Type
MHC-LC773 A*0201/Human EGFR (YLNTVQPTCV) MHC Tetramer FCM
MHC-LC4545 PE-DQB1*03:02/Human EGFR (SRALEEKKGNYVVTHG) MHC Tetramer FCM
MHC-YF409 A*02:01/Human EGF-R-479 (KLFGTSGQKT) MHC Pentamer FCM

Neutralizing Antibody

CAT Product Name Application Type
NEUT-722CQ Rabbit Anti-EGFR Recombinant Antibody (clone CBL1011) Neut Rabbit IgG
NEUT-723CQ Mouse Anti-EGFR Recombinant Antibody (clone CBL931) WB, IP, IHC, ICC, Neut Mouse IgG1
NEUT-724CQ Rabbit Anti-EGFR Recombinant Antibody (clone D1D4J) IF, FC, WB, IP, Neut Rabbit IgG

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1101 Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone DS1101AB) IHC-P Rabbit IgG
MOR-4520 Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH28DS) IF, ICC, FC Rabbit IgG
MOR-4570 Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH82DS) ELISA Rabbit IgG
MOR-4571 Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH83DS) WB, IF, ICC, FC Rabbit IgG
MOR-0033-FY Rabbit Anti-EGFR Recombinant Antibody (clone AFY0004) ICC, IHC, WB Rabbit IgG

Fab Fragment Antibody

CAT Product Name Application Type
HPAB-1443LY-F(E) Human Anti-EGFR Recombinant Antibody (clone P1X); Fab Fragment ELISA, WB Human Fab
HPAB-1444LY-F(E) Human Anti-EGFR Recombinant Antibody (clone P2X); Fab Fragment ELISA, WB Human Fab
HPAB-1445LY-F(E) Human Anti-EGFR Recombinant Antibody (clone P3X); Fab Fragment ELISA, WB Human Fab
HPAB-1446LY-F(E) Human Anti-EGFR Recombinant Antibody (clone 25E); Fab Fragment ELISA, WB Human Fab
HPAB-0136-YJ-F(E) Human Anti-EGFR Recombinant Antibody; Fab Fragment (HPAB-0136-YJ-F(E)) ELISA, FC, Inhib, FuncS Human Fab

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-710 Afuco™ Anti-EGFR ADCC Recombinant Antibody (Nimotuzumab), ADCC Enhanced ELISA, IP, FC, FuncS, Neut, IF ADCC enhanced antibody
AFC-TAB-753 Afuco™ Anti-EGFR ADCC Recombinant Antibody (Imgatuzumab), ADCC Enhanced Neut, ELISA, IF, IP, FuncS, FC ADCC enhanced antibody
AFC-TAB-H35 Afuco™ Anti-EGFR ADCC Recombinant Antibody (Futuximab), ADCC Enhanced FuncS, IF, Neut, ELISA, FC, IP ADCC enhanced antibody
AFC-TAB-H49 Afuco™ Anti-EGFR ADCC Recombinant Antibody (Modotuximab), ADCC Enhanced FuncS, IF, Neut, ELISA, FC, IP ADCC enhanced antibody
AFC-TAB-020 Afuco™ Anti-EGFR ADCC Recombinant Antibody (Panitumumab), ADCC Enhanced ELISA, IP, FC, FuncS, Neut, IF ADCC enhanced antibody

scFv Fragment Antibody

CAT Product Name Application Type
HPAB-2059-FY-S(P) Mouse Anti-EGFR Recombinant Antibody (clone L2-12B); scFv Fragment FC, ELISA Mouse scFv
HPAB-2060-FY-S(P) Mouse Anti-EGFR Recombinant Antibody (clone L3-11D); scFv Fragment FC, ELISA Mouse scFv
HPAB-2098-FY-S(P) Mouse Anti-EGFR Recombinant Antibody (clone Mint5); scFv Fragment ICC, Inhib Mouse scFv
HPAB-1530WJ-S(P) Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (clone 340) ELISA, WB Mouse scFv
HPAB-1531WJ-S(P) Human Anti-EGFR Recombinant Antibody; scFv Fragment (clone hu340) ELISA, WB Human scFv

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
WBWestern Blot

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