Human Anti-EGFR Recombinant Antibody (clone C225) (CAT#: PABL-462)

Recombinant Human Antibody (C225) is capable of binding to EGFR, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-EGFR mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-EGFR proteins mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition.

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Figure 1 Inhibition of VEGF (A), IL-8 (A), and bFGF (B) steady-state mRNA expression by C225 mAb in vitro.

Figure 1 Inhibition of VEGF (A), IL-8 (A), and bFGF (B) steady-state mRNA expression by C225 mAb in vitro.

Cells were cultured in the presence of or absence of MAb C225. mRNA was extracted after 48 h of treatment and analyzed by Northern blotting. Using image densitometry, the mRNA expression of untreated cells was assumed to be 100% of potential expression for these cells. Treatment with C225 down-regulated VEGF, IL-8, and bFGF mRNA expression in a dose-dependent fashion. β-actin served as a control for loading (exposure, 30 min).

Perrotte, P., Matsumoto, T., Inoue, K., Kuniyasu, H., Eve, B. Y., Hicklin, D. J., ... & Dinney, C. P. (1999). Anti-epidermal growth factor receptor antibody C225 inhibits angiogenesis in human transitional cell carcinoma growing orthotopically in nude mice. Clinical Cancer Research, 5(2), 257-264.

Figure 2 Immunohistochemical staining of MAb C225-treated human TCC tumors growing in the bladders of athymic nude mice.

Figure 2 Immunohistochemical staining of MAb C225-treated human TCC tumors growing in the bladders of athymic nude mice.

MAb C225-treated tumors (after 3 weeks and 5 weeks of therapy) and control tumors were stained with CD31 antibodies directed against mouse endothelial cells (A–C). Note the increased vessel density in the control tumors (A) and the 3-week C225-treated tumors (B) compared with that of the 5-week C225-treated specimen (C). Immunostaining for VEGF (D–F), IL-8 (G–I), and bFGF (J–L) is higher in the control tumors (D, G, and J) than in the MAb C225-treated specimens (E, F, H, I, K, and L) at 3 weeks and 5 weeks. Note that the down-regulation of VEGF, IL-8, and bFGF precedes the inhibition of angiogenesis.

Perrotte, P., Matsumoto, T., Inoue, K., Kuniyasu, H., Eve, B. Y., Hicklin, D. J., ... & Dinney, C. P. (1999). Anti-epidermal growth factor receptor antibody C225 inhibits angiogenesis in human transitional cell carcinoma growing orthotopically in nude mice. Clinical Cancer Research, 5(2), 257-264.


Specifications

  • Immunogen
  • Human epidermal growth factor receptor
  • Host Species
  • Human
  • Type
  • Human IgG
  • Specificity
  • Human EGFR
  • Species Reactivity
  • Human
  • Clone
  • C225
  • Applications
  • FC

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • EGFR; epidermal growth factor receptor; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2; proto-oncogene c-ErbB-1; cell growth inhibiting protein 40; erb-b2 receptor tyrosine kinase 1; cell proliferation-inducing protein 61; receptor tyrosine-protein kinase erbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog;

Related Resources

  • Related Diseases
  • Related Signaling Pathways

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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