Human Anti-EGFR Recombinant Antibody (clone MR1) (CAT#: PABC-040)

Recombinant Human Antibody (MR1) is capable of binding to EGFRvIII dodecapeptide (EEKKGNYVVTDH), expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-EGFRvIII dodecapeptide (EEKKGNYVVTDH) mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-EGFRvIII dodecapeptide (EEKKGNYVVTDH) mAb and CL of human kappa light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. Necitumumab is a recombinant human IgG1 monoclonal antibody that binds to the human epidermal growth factor receptor (EGFR) and blocks the binding of EGFR to its ligands. IMC-11F8 is a fully human antibody that has similar antitumor potency as the chimeric cetuximab/Erbitux and might represent a safer therapeutic alternative.

Host: Human

Isotype: Human IgG

Applications: Block


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WB

Figure 1 Epidermal growth factor receptor variant III (EGFRvIII) recognition by different antibodies.

(A) Lysates of EGFRvIII-transfected and parental A431 and HEK293T cells were analyzed by immunoblot using the stated antibodies for detection and b-actin as a loading control. Arrows indicate the expected molecular weights of wild-type (wt) EGFR and EGFRvIII, which appears as a double band. 13.1.2 exclusively detected EGFRvIII, whereas ch806 and MR1-1 also bound to wt EGFR on nontransfected cells. (B) Indirect immunofluorescence revealed a similar binding of EGFRvIII antibodies to EGFRvIII-expressing A431 (A431-vIII) and HEK293T (HEK293T-vIII) cells (lower panel). Additional binding to A431 cells was obtained with MR1-1 (grey line) and ch806 (black line). MR1-1 also displayed reactivity with HEK293T cells, whereas 13.1.2 (dotted line) only bound to EGFRvIIItransfected cells. Zalutumumab (black fill) recognized wt EGFR and EGFRvIII. An irrelevant IgG1 antibody (KLH) served as a control (grey fill).

Klausz, K. , Berger, S. , Bueren, J. J. L. V. , Derer, S. , Lohse, S. , & Dechant, M. , et al. (2011). Complement-mediated tumor-specific cell lysis by antibody combinations targeting epidermal growth factor receptor (egfr) and its variant iii (egfrviii). Cancer Science, 102(10), 1761-1768.


Specifications

  • Immunogen
  • Human epidermal growth factor receptor
  • Host Species
  • Human
  • Type
  • Human IgG
  • Specificity
  • Human EGFR
  • Species Reactivity
  • Human
  • Clone
  • MR1
  • Applications
  • Block

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The antibody MR1 has been reported in applications of Blocking, Western Blot, Immunofluorescence, Flow Cytometry. It's recommended that the optimal antibody concentration, dilution, incubition time etc. be carefully titrated in specific assays.

Target

  • Alternative Names
  • EGFR; epidermal growth factor receptor; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2; proto-oncogene c-ErbB-1; cell growth inhibiting protein 40; erb-b2 receptor tyrosine kinase 1; cell proliferation-inducing protein 61; receptor tyrosine-protein kinase erbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog

Related Resources

  • Related Diseases
  • Related Signaling Pathways

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Recombinant Antibody

CAT Product Name Application Type
MOB-1078z Mouse Anti-EGFR Recombinant Antibody (clone 42C11) WB, ELISA, FC, IF, IHC, FuncS Mouse IgG1, κ
PABZ-039 Mouse Anti-EGFR Recombinant Antibody (clone mAb528) FC Mouse IgG
PABW-039 Human Anti-EGFR Recombinant Antibody (clone Cetuximab (IMC-C225)) FuncS Chimeric (mouse/human) IgG
PABW-171 Mouse Anti-EGFR Recombinant Antibody (PABW-171) WB Mouse IgG
PSBW-088 Mouse Anti-EGFR Recombinant Antibody (clone 7A7); scFv Fragment ELISA, WB Mouse scFv

Single-domain Antibody

CAT Product Name Application Type
NABG-056 Recombinant Anti-Mouse Egfr VHH Single Domain Antibody ELISA, IHC, FC, FuncS Llama VHH
PNBL-016 Recombinant Anti-Human EGFR VHH Single Domain Antibody (7D12) WB, ELISA Llama VHH
PNBL-017 Recombinant Anti-Human EGFR VHH Single Domain Antibody (9G8) FuncS, ELISA, IF Llama VHH
PNBL-018 Recombinant Anti-Human EGFR VHH Single Domain Antibody (EgA1) FuncS, SPR Llama VHH
TAB-015MZ-VHH Anti-Human EGFR Recombinant Antibody (PMP7A5) sELISA Single domain antibody

Chimeric Antibody

CAT Product Name Application Type
TAB-H35 Anti-Human EGFR Recombinant Antibody (Futuximab) IF, WB, Inhib IgG1 - kappa
TAB-308MZ Human Anti-EGFR Recombinant Antibody (TAB-308MZ) FuncS Chimeric antibody (mouse/human)
TAB-272MZ-S(P) Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-272MZ-S(P)) FuncS Mouse scFv
TAB-302MZ-S(P) Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-302MZ-S(P)) ELISA Mouse scFv
TAB-308MZ-S(P) Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-308MZ-S(P)) ELISA, FC Mouse scFv

Humanized Antibody

CAT Product Name Application Type
TAB-165 Anti-Human EGFR Recombinant Antibody (Matuzumab) Neut, ELISA, IF, IP, FuncS, FC, ICC IgG1
TAB-710 Anti-EGFR Recombinant Antibody (Nimotuzumab) ELISA, IP, FC, FuncS, Neut, IF, IHC IgG1 - kappa
TAB-274MZ Human Anti-EGFR Recombinant Antibody (TAB-274MZ) FC Humanized IgG
TAB-274MZ-S(P) Human Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-274MZ-S(P)) FC Humanized scFv
TAB-274MZ-F(E) Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-274MZ-F(E)) FC Humanized Fab

scFv Fragment Antibody

CAT Product Name Application Type
PSBL-080 Human Anti-EGFR Recombinant Antibody; scFv Fragment (PSBL-080) ELISA, WB, FuncS Human scFv
HPAB-3391LY-S(P) Human Anti-EGFR Recombinant Antibody (clone CBL154); scFv Fragment Inhib Human scFv
HPAB-1519-FY-F(E) Human Anti-EGFR Recombinant Antibody (clone 15H8); scFv Fragment ELISA Human scFv
HPAB-1158WJ-S(P) Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (HPAB-1158WJ-S(P)) ELISA Mouse scFv
HPAB-S0021-YC-S(P) Human Anti-EGFR Recombinant Antibody (clone E6.3); scFv Fragment ELISA, Inhib Human scFv

Fab Fragment Antibody

CAT Product Name Application Type
PFBZ-039 Mouse Anti-EGFR Recombinant Antibody (clone mAb528); Fab Fragment FC Mouse Fab
PFBW-039 Human Anti-EGFR Recombinant Antibody (clone Cetuximab (IMC-C225)); Fab Fragment FuncS Chimeric (mouse/human) Fab
PFBC-040 Human Anti-EGFR Recombinant Antibody (clone MR1); Fab Fragment Block Human Fab
PFBL-459 Human Anti-EGFR Recombinant Antibody (clone C225); Fab Fragment FC Human Fab
PFBL-460 Human Anti-EGFR Recombinant Antibody (clone h-R3); Fab Fragment ELISA, WB, FuncS Humanized Fab

Mouse Antibody

CAT Product Name Application Type
TAB-303MZ-F(E) Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-303MZ-F(E)) ELISA Mouse Fab
TAB-304MZ-F(E) Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-304MZ-F(E)) ELISA Mouse Fab
TAB-305MZ-F(E) Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-305MZ-F(E)) ELISA Mouse Fab
TAB-306MZ-F(E) Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-306MZ-F(E)) ELISA Mouse Fab
TAB-326MZ-F(E) Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-326MZ-F(E)) FC Mouse Fab

Human Antibody

CAT Product Name Application Type
TAB-321MZ-F(E) Anti-Human EGFR Recombinant Antibody Fab Fragment (DX 1-4) SPR Human antibody
TAB-322MZ-F(E) Anti-Human EGFR Recombinant Antibody Fab Fragment (DX 1-6) SPR Human antibody
TAB-323MZ-F(E) Anti-Human EGFR Recombinant Antibody Fab Fragment (DX 1-10) SPR Human antibody
TAB-324MZ-F(E) Anti-Human EGFR Recombinant Antibody Fab Fragment (pSEX81-6) IP, ELISA, FC Human antibody
TAB-325MZ-F(E) Anti-Human EGFR Recombinant Antibody Fab Fragment (pSEX81-63) IP, ELISA, FC Human antibody

Fc Glycosylation

CAT Product Name Application Type
Gly-055LC Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated) ELISA Chimeric antibody (mouse/human)
Gly-144LC Recombinant Anti-Human EGFR Antibody (Fc glycosylation) ELISA Humanized antibody

High-mannose Glycoform

CAT Product Name Application Type
Gly-055LC-1 Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated) ELISA Chimeric antibody (mouse/human)

Deglycosylated Antibody (Non-glycosylated IgGs)

CAT Product Name Application Type
Gly-167LC Recombinant Anti-Human EGFR Antibody (Non-glycosylated) ELISA Human antibody

Chicken IgY Antibody

CAT Product Name Application Type
BRD-0183MZ Chicken Anti-EGFR Polyclonal IgY WB Chicken antibody
BRD-0668MZ Chicken Anti-EGFR Polyclonal IgY WB Chicken antibody

MHC Tetramer for Cancer

CAT Product Name Application Type
MHC-LC773 A*0201/Human EGFR (YLNTVQPTCV) MHC Tetramer FCM
MHC-LC4545 PE-DQB1*03:02/Human EGFR (SRALEEKKGNYVVTHG) MHC Tetramer FCM
MHC-YF409 A*02:01/Human EGF-R-479 (KLFGTSGQKT) MHC Pentamer FCM

Neutralizing Antibody

CAT Product Name Application Type
NEUT-722CQ Rabbit Anti-EGFR Recombinant Antibody (clone CBL1011) Neut Rabbit IgG
NEUT-723CQ Mouse Anti-EGFR Recombinant Antibody (clone CBL931) WB, IP, IHC, ICC, Neut Mouse IgG1
NEUT-724CQ Rabbit Anti-EGFR Recombinant Antibody (clone D1D4J) IF, FC, WB, IP, Neut Rabbit IgG

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1101 Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone DS1101AB) IHC-P Rabbit IgG
MOR-4520 Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH28DS) IF, ICC, FC Rabbit IgG
MOR-4570 Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH82DS) ELISA Rabbit IgG
MOR-4571 Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH83DS) WB, IF, ICC, FC Rabbit IgG
MOR-0033-FY Rabbit Anti-EGFR Recombinant Antibody (clone AFY0004) ICC, IHC, WB Rabbit IgG

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-165 Afuco™ Anti-EGFR ADCC Recombinant Antibody (Matuzumab), ADCC Enhanced Neut, ELISA, IF, IP, FuncS, FC ADCC enhanced antibody
AFC-TAB-464CQ Afuco™ Anti-EGFR ADCC Recombinant Antibody (Tomuzotuximab), ADCC Enhanced ELISA, IHC, FC, IP, IF, FuncS ADCC enhanced antibody
AFC-TAB-003 Afuco™ Anti-EGFR ADCC Recombinant Antibody (Cetuximab), ADCC Enhanced IF, IP, Neut, FuncS, ELISA, FC ADCC enhanced antibody
AFC-TAB-710 Afuco™ Anti-EGFR ADCC Recombinant Antibody (Nimotuzumab), ADCC Enhanced ELISA, IP, FC, FuncS, Neut, IF ADCC enhanced antibody
AFC-TAB-753 Afuco™ Anti-EGFR ADCC Recombinant Antibody (Imgatuzumab), ADCC Enhanced Neut, ELISA, IF, IP, FuncS, FC ADCC enhanced antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
WBWestern Blot

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