Anti-Human HMG1 Recombinant Antibody (TAB-100CT) (CAT#: TAB-100CT)
Recombinant monoclonal antibody to HMG1. This antibody intended for the prophylaxis and treatment of HMGB1-related diseases .
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line RH-30
(Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73663/2043_F12_32_selected.jpg
(Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73663/2043_F12_32_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5873/617_A1_1_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5873/617_A1_1_selected.jpg
Figure 3 IHC staining of human bone marrow
(Immunohistochemical staining of human bone marrow shows strong nuclear positivity in hematopoietic cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_B_4_4_selected.jpg
(Immunohistochemical staining of human bone marrow shows strong nuclear positivity in hematopoietic cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_B_4_4_selected.jpg
Figure 4 IHC staining of human esophagus
(Immunohistochemical staining of human esophagus shows strong nuclear positivity in squamous epithelial cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5873/ihc_selected.jpg
(Immunohistochemical staining of human esophagus shows strong nuclear positivity in squamous epithelial cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5873/ihc_selected.jpg
Figure 5 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5873/613_A1_1_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5873/613_A1_1_red_green.jpg
Figure 6 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5873/610_A1_1_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5873/610_A1_1_red_green.jpg
Figure 7 Cerebral cortex
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_B_8_5.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_B_8_5.jpg
Figure 8 Colon
(Endocrine cells
Staining: High
Intensity: Strong
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_A_9_3.jpg
(Endocrine cells
Staining: High
Intensity: Strong
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_A_9_3.jpg
Figure 9 Liver
(Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_A_8_4.jpg
(Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_A_8_4.jpg
Figure 10 Kidney
(Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_A_7_5.jpg
(Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_A_7_5.jpg
Figure 11 Lymph node
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_A_8_8.jpg
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_A_8_8.jpg
Figure 12 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000189403-HMGB1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000189403-HMGB1
Figure 12 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000189403-HMGB1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000189403-HMGB1
Specifications
- Immunogen
- 17-mer peptide P1 (KGKPDAAKKGVVKAEKS)
- Host Species
- Mouse
- Derivation
- Hybridoma cell line
- Specificity
- Human
- Species Reactivity
- Human
- Applications
- WB, IF, FC, FuncS, Transmigration assay
- Related Disease
- HMGB1-related diseases
Applications
- Application Notes
- Anti-HMGB-1 Ab (DPH1.1 Ab) characterization and specificity testing by Western blot
Western blots of the recombinant BoxA domain of HMGB1 (negative control) incubated with either the anti-HMGB-1 mAb DPH1.1 or a control anti-BoxA mAb control were performed. Briefly, 500 ng or 100 ng of recombinant HMGB1 and the (negative control) recombinant BoxA domain of HMGB1 were separated by gel electrophoresis and transferred onto membranes.
Immunofluorescence
DPH1.1 mAb, anti-BoxA mAb and goat anti- mouse lgG1 Ab were applied at 1μg/ml dilution. Immunofluorescence was performed on mouse embryonic fibroblasts (MEFs) derived from either wild type mice or HmgbV¹ mice. DPH1.1 mAb and AlexaFluor 633-labelled goat anti-mouse lgG1 Ab were applied at a 50 μg/ml dilution.
Transmigration assay
Recombinant HMGB1 was added to the lower chamber at the concentration of 30 ng/ml. Increasing concentrations of DPH1. 1 mAb (or of an irrelevant isotype-matched mAb) were added to fifty thousand 3T3 cells. Cell migration was assessed by a modified Boyden chamber assay. Boyden chambers were incubated at 37 °C in 5% CO₂ for 3 hrs. Cells remaining on the upper section of the filters were removed mechanically. Cells that migrated to the lower section of the filters were fixed with ethanol, stained with Giemsa, and counted in 10 random fields/filter.
In vivo experiments
Inflammatory cell recruitment Recruitment of inflammatory cells can be amplified by removal of Kupffer cells (KCs) from the liver, which can be obtained by treatment of the mice with clodronate (Clo-L). In order to amplify the effect of HMGB1, Kupffer cells (KCs) of HBV transgenic mice were removed, and injected with CD8 T cells from mice immunized against HBV (this causes acute hepatitis in the mice).
Target
- Alternative Names
- HMGB1; high mobility group box 1; HMG1; HMG3; SBP-1; high mobility group protein B1; HMG-1; Amphoterin; high-mobility group box 1; high mobility group protein 1; Sulfoglucuronyl carbohydrate binding protein; high-mobility group (nonhistone chromosomal) protein 1; anti-HMGB1
- Gene ID
- 3146
- UniProt ID
- A0A024RDR0
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "HMGB1"
Select a product category from the dropdown menu below to view related products.
Please select product type
IgG Antibody Products
Mouse Antibody Products
Humanized Antibody Products
Human Antibody Products
Rabbit Monoclonal Antibody Products
MHC Tetramer Products for Cancer
ScFv Antibody Products
Fab Antibody Products
IgM Antibody Products
Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-956 | Human Anti-HMGB1 Recombinant Antibody (clone Giby-1-4) | ELISA, WB | Human IgG |
| DrMAB-957 | Human Anti-HMGB1 Recombinant Antibody (clone Giby-1-4), Biotin | ELISA, WB | Human IgG |
| MRO-0755-CN | Rabbit Anti-HMGB1 Recombinant Antibody (clone CBACN-279) | WB, IF, IHC, FC | Rabbit IgG |
| ZG-0740J | Rabbit Anti-HMGB1 Recombinant Antibody (clone 1A1) | ELISA, WB, IHC | Rabbit IgG |
| VS3-WK1655 | Recombinant Mouse Anti-HMGB1 Detection Antibody (clone 1G1) | ELISA | Mouse IgG2b |
Customer Reviews and Q&As
Customer Review
Q&As
Optimal for Neurological Studies
The Anti-Human HMG1 Recombinant Antibody from Creative Biolabs excels in neurological research. Its high sensitivity allows for precise detection of HMGB1, enhancing the understanding of its role in neuroinflammation and brain injuries.
Reliable Results
This antibody demonstrates exceptional consistency in immunology research. Its superior binding affinity and specificity ensure accurate results in studying the role of HMGB1 in immune responses.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-100CT, RRID: AB_3111806)
Copy citation
Popular products with customers
Application: FuncS, IF, Neut, ELISA, FC, IP, IHC
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
-1.png)
Application: ELISA, FC, Block, SPR
-1.png)
Application: WB, ELISA
Application: ELISA, FC, IF, WB
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, FC, WB, Inhib, IHC
Application: ELISA, Vaccine, FuncS
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
