1 0
Cart
    Inquiry
    Popular Searches: Recombinant Antibody MHC Tetramer T cell Receptor Scaffold Protein

    Anti-Human HMG1 Recombinant Antibody (TAB-100CT) (CAT#: TAB-100CT)

    Recombinant monoclonal antibody to HMG1. This antibody intended for the prophylaxis and treatment of HMGB1-related diseases .

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
    Modalities :
    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line RH-30
    Figure 1 IF staining of human cell line RH-30
    (Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73663/2043_F12_32_selected.jpg
    Figure 2 IF staining of human cell line A-431
    Figure 2 IF staining of human cell line A-431
    (Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5873/617_A1_1_selected.jpg
    Figure 3 IHC staining of human bone marrow
    Figure 3 IHC staining of human bone marrow
    (Immunohistochemical staining of human bone marrow shows strong nuclear positivity in hematopoietic cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_B_4_4_selected.jpg
    Figure 4 IHC staining of human esophagus
    Figure 4 IHC staining of human esophagus
    (Immunohistochemical staining of human esophagus shows strong nuclear positivity in squamous epithelial cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5873/ihc_selected.jpg
    Figure 5 IF staining of human cell line U-2 OS
    Figure 5 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5873/613_A1_1_red_green.jpg
    Figure 6 IF staining of human cell line U-251 MG
    Figure 6 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5873/610_A1_1_red_green.jpg
    Normal Tissue
    Figure 7 Cerebral cortex
    Figure 7 Cerebral cortex
    (Endothelial cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear
    Glial cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_B_8_5.jpg
    Figure 8 Colon
    Figure 8 Colon
    (Endocrine cells
    Staining: High
    Intensity: Strong
    Endothelial cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Enterocytes
    Staining: High
    Intensity: Strong
    Quantity:>75%)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_A_9_3.jpg
    Figure 9 Liver
    Figure 9 Liver
    (Cholangiocytes
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Hepatocytes
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_A_8_4.jpg
    Figure 10 Kidney
    Figure 10 Kidney
    (Cells in glomeruli
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Cells in tubules
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_A_7_5.jpg
    Figure 11 Lymph node
    Figure 11 Lymph node
    (Germinal center cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Non-germinal center cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_A_8_8.jpg
    RNA Expression
    Figure 12 RNA cell line category: Low cell line specificity
    Figure 12 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000189403-HMGB1
    Figure 12 RNA cell line category: Low cell line specificity
    Figure 12 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000189403-HMGB1

    Specifications

    • Immunogen
    • 17-mer peptide P1 (KGKPDAAKKGVVKAEKS)
    • Host Species
    • Mouse
    • Derivation
    • Hybridoma cell line
    • Specificity
    • Human
    • Species Reactivity
    • Human
    • Applications
    • WB, IF, FC, FuncS, Transmigration assay
    • Related Disease
    • HMGB1-related diseases

    Applications

    • Application Notes
    • Anti-HMGB-1 Ab (DPH1.1 Ab) characterization and specificity testing by Western blot
      Western blots of the recombinant BoxA domain of HMGB1 (negative control) incubated with either the anti-HMGB-1 mAb DPH1.1 or a control anti-BoxA mAb control were performed. Briefly, 500 ng or 100 ng of recombinant HMGB1 and the (negative control) recombinant BoxA domain of HMGB1 were separated by gel electrophoresis and transferred onto membranes.
      Immunofluorescence
      DPH1.1 mAb, anti-BoxA mAb and goat anti- mouse lgG1 Ab were applied at 1μg/ml dilution. Immunofluorescence was performed on mouse embryonic fibroblasts (MEFs) derived from either wild type mice or HmgbV¹ mice. DPH1.1 mAb and AlexaFluor 633-labelled goat anti-mouse lgG1 Ab were applied at a 50 μg/ml dilution.
      Transmigration assay
      Recombinant HMGB1 was added to the lower chamber at the concentration of 30 ng/ml. Increasing concentrations of DPH1. 1 mAb (or of an irrelevant isotype-matched mAb) were added to fifty thousand 3T3 cells. Cell migration was assessed by a modified Boyden chamber assay. Boyden chambers were incubated at 37 °C in 5% CO₂ for 3 hrs. Cells remaining on the upper section of the filters were removed mechanically. Cells that migrated to the lower section of the filters were fixed with ethanol, stained with Giemsa, and counted in 10 random fields/filter.
      In vivo experiments
      Inflammatory cell recruitment Recruitment of inflammatory cells can be amplified by removal of Kupffer cells (KCs) from the liver, which can be obtained by treatment of the mice with clodronate (Clo-L). In order to amplify the effect of HMGB1, Kupffer cells (KCs) of HBV transgenic mice were removed, and injected with CD8 T cells from mice immunized against HBV (this causes acute hepatitis in the mice).

    Target

    • Alternative Names
    • HMGB1; high mobility group box 1; HMG1; HMG3; SBP-1; high mobility group protein B1; HMG-1; Amphoterin; high-mobility group box 1; high mobility group protein 1; Sulfoglucuronyl carbohydrate binding protein; high-mobility group (nonhistone chromosomal) protein 1; anti-HMGB1

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "HMGB1"

    Select a product category from the dropdown menu below to view related products.
    Please select product type
    IgG Antibody Products Mouse Antibody Products Humanized Antibody Products Human Antibody Products Rabbit Monoclonal Antibody Products MHC Tetramer Products for Cancer ScFv Antibody Products Fab Antibody Products IgM Antibody Products Antibody Magnetic Beads IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research
    CAT Product Name Application Type
    DrMAB-956 Human Anti-HMGB1 Recombinant Antibody (clone Giby-1-4) ELISA, WB Human IgG
    DrMAB-957 Human Anti-HMGB1 Recombinant Antibody (clone Giby-1-4), Biotin ELISA, WB Human IgG
    HPAB-0101-WJ Human Anti-HMGB1 Recombinant Antibody (HPAB-0101-WJ) ELISA, WB Humanized IgG1, λ
    VS3-CJ537 Rabbit Anti-HMGB1 Recombinant Antibody (VS3-CJ537) WB, ICC, IF, IHC, FC Rabbit IgG
    VS-0824-CJ6 Human Anti-HMGB1 Recombinant Antibody (clone G16) ELISA Human IgG

    Customer Reviews and Q&As

    Customer Review Q&As

    Submit a review or a question


    Optimal for Neurological Studies
    The Anti-Human HMG1 Recombinant Antibody from Creative Biolabs excels in neurological research. Its high sensitivity allows for precise detection of HMGB1, enhancing the understanding of its role in neuroinflammation and brain injuries.

    Reliable Results
    This antibody demonstrates exceptional consistency in immunology research. Its superior binding affinity and specificity ensure accurate results in studying the role of HMGB1 in immune responses.

    Cite This Product

    To accurately reference this product in your publication, please use the following citation information:

    (Creative Biolabs Cat# TAB-100CT, RRID: AB_3111806)

    Copy citation

    Popular products with customers

    Figure 3 Anti-Human CD20 Recombinant Antibody (TAB-016) in ELISA

    Anti-Human CD20 Recombinant Antibody (Rituximab) (CAT# TAB-016)
    Application: IF, IP, Neut, FuncS, ELISA, FC, WB

    Figure 3 Anti-IL12+IL23 Antibody (TAB-103) in Dot Blot

    Anti-Human IL12+IL23 Recombinant Antibody (Briakinumab) (CAT# TAB-103)
    Application: WB, FC, IP, ELISA, Neut, FuncS, IF

    Figure 1 Anti-ERBB2 Recombinant Antibody (Margetuximab) (TAB-761) in SPR

    Anti-ERBB2 Recombinant Antibody (Margetuximab) (CAT# TAB-761)
    Application: IF, IP, Neut, FuncS, ELISA, FC, WB

    Figure 2 Anti-EGFR Recombinant Antibody (TAB-753) in HPLC

    Anti-EGFR Recombinant Antibody (Imgatuzumab) (CAT# TAB-753)
    Application: Neut, ELISA, IF, IP, FuncS, FC, WB

    Figure 2 Anti-Human NGF Recombinant Antibody (TAB-111) in ELISA

    Anti-Human NGF Recombinant Antibody (TAB-111) (CAT# TAB-111)
    Application: ELISA, FuncS, Inhib

    Figure 1 Anti-Human CD70 Recombinant Antibody (TAB-H76) in ELISA

    Anti-Human CD70 Recombinant Antibody (Vorsetuzumab) (CAT# TAB-H76)
    Application: ELISA, FC, IP, FuncS, IF, Neut, ICC

    Figure 2 Anti-Influenza Virus M2e Antibody (TAB-508CL) in HPLC

    Anti-Influenza Virus M2e Recombinant Antibody (TCN-032) (CAT# TAB-508CL)
    Application: FuncS

    Figure 2 Recombinant Mouse Anti-uPAR Antibody (PABL-355) in ELISA

    Mouse Anti-PLAUR Recombinant Antibody (PABL-355) (CAT# PABL-355)
    Application: WB, ELISA, FuncS

    Figure 3 Mouse Anti-Il2 Recombinant Antibody (PABL-609) in DB

    Mouse Anti-Il2 Recombinant Antibody (PABL-609) (CAT# PABL-609)
    Application: FC, FuncS, IHC, ELISA

    Figure 3 Anti-CD33 Antibody (TAB-119LC) in DB

    Mouse Anti-CD33 Recombinant Antibody (TAB-119LC) (CAT# TAB-119LC)
    Application: ELISA, IF, RIA

    Figure 2 Anti-Human DEspR Recombinant Antibody (TAB-190MZ) in HPLC

    Anti-Human DEspR Recombinant Antibody (TAB-190MZ) (CAT# TAB-190MZ)
    Application: FC, FuncS, Inhib

    Figure 2 Anti-Mouse ANGPTL4 Recombinant Antibody (TAB-0913CLV) in HPLC

    Mouse Anti-ANGPTL4 Recombinant Antibody (TAB-0913CLV) (CAT# TAB-0913CLV)
    Application: ELISA, WB

    Figure 2 Mouse Anti-ERBB2 Recombinant Antibody (NEUT-737CQ) in HPLC

    Mouse Anti-ERBB2 Recombinant Antibody (clone 7.16.4) (CAT# NEUT-737CQ)
    Application: Block, IP, IF, FC

    Figure 3 Anti-Human ENG Antibody (TAB-013ML) in DB

    Anti-Human ENG Recombinant Antibody (TAB-013ML) (CAT# TAB-013ML)
    Application: ELISA, IHC, FC, IP, IF, Inhib

    Figure 3 Anti-MAPT Recombinant Antibody (NS-090CN) in ELISA

    Mouse Anti-MAPT Recombinant Antibody (NS-090CN) (CAT# NS-090CN)
    Application: WB, ELISA, IHC, IP, SPR, FuncS

    Figure 4 Mouse Anti-PSCA Recombinant Antibody (HPAB-0287CQ) in WB

    Mouse Anti-PSCA Recombinant Antibody (clone mAb 8D11) (CAT# HPAB-0287CQ)
    Application: FC, IHC, Cyt, FuncS

    Figure 4 Mouse Anti-C5 Recombinant Antibody Fab Fragment (HPAB-0128CQ-F(E)) in ELISA

    Mouse Anti-C5 Recombinant Antibody (clone BB5.1); Fab Fragment (CAT# HPAB-0128CQ-F(E))
    Application: FC, FuncS, IA, IF, IP, IHC

    Figure 2 Human Anti-APS Recombinant Antibody (clone CL1) (HPAB-1960-FY) in HPLC

    Human Anti-APS Recombinant Antibody (clone CL1) (CAT# HPAB-1960-FY)
    Application: ELISA, Activ, Block

    Figure 4 Human Anti-Rotavirus VP8* Recombinant Antibody (clone mAb9) (PABJ-0170) in DB

    Human Anti-Rotavirus VP8* Recombinant Antibody (clone mAb9) (CAT# PABJ-0170)
    Application: ELISA, Neut

    Figure 3 Mouse Anti-SOX9 Antibody (clone ABT-SOX9) in WB

    Mouse Anti-SOX9 Recombinant Antibody (clone ABT-SOX9) (CAT# ZG-0104C)
    Application: IHC-P, IF

    For Research Use Only. Not For Clinical Use.

    For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

    Send Inquiry

    This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

    Go to compare

    Go to compare