Anti-Human EGFR Recombinant Antibody (Necitumumab)

Tested Data
Gene Expression
Datasheet
MSDS
Publications
COA

ELISA

Figure 1 Anti-Human EGFR Antibody in ELISA

ELISA analysis of HPAB-0109-YJ was performed by coating with Recombinant Biotin Protein. Then blocked with BSA, and incubated with Anti-Human EGFR Antibody (TAB-119). The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Subcellular Location and Protein Expression

Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cell junctions.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/144_E9_2_selected.jpg

Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/143_E9_1_blue_red_green.jpg

Normal Tissue

Figure 3 Colon
(Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_3.jpg

Figure 4 Liver
(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_4.jpg

Figure 5 Kidney
(Bowman's capsule
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Collecting ducts
Staining:Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Proximal tubules (cell body)
Staining:Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_5.jpg

Figure 6 Testis
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_6_6.jpg

Figure 7 Placenta
(Cytotrophoblasts
Staining:High
Intensity: Strong
Quantity:>75%
Decidual cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Hofbauer cells
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - cell body
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_1_7.jpg

Figure 8 Lymph node
(Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_8.jpg

RNA Expression

Figure 9 RNA cell line category: Cell line enriched (A-431)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000146648-EGFR

Datasheet

MSDS

Peer-reviewed Publications

Our products have been used extensively by scientists all over the world to accelerate their life science research. Here, we present a collection of publications that cite this product.

Lu, Xinmiao, et al. "Radiolabeling and biological evaluation of 125 I-Necitumumab for EGFR-targeted SPECT imaging." Journal of Radioanalytical and Nuclear Chemistry (2019): 1-7.

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

CAT	Product Name	Application	Type
PABL-462	Human Anti-EGFR Recombinant Antibody (clone C225)	FC	Human IgG
PABL-463	Human Anti-EGFR Recombinant Antibody (clone h-R3)	ELISA, WB, FuncS	Humanized IgG
MOB-0094MZ	Recombinant Mouse Anti-EGFR Antibody (clone FGF-R2)	ELISA, IHC-P, FC	Mouse antibody
HPAB-0010-YC	Mouse Anti-EGFR Recombinant Antibody (clone L211C)	ELISA, FC	Mouse IgG
HPAB-0011-YC	Human Anti-EGFR Recombinant Antibody (clone Pep 1)	FC	Human IgG

CAT	Product Name	Application	Type
PFBL-459	Human Anti-EGFR Recombinant Antibody (clone C225); Fab Fragment	FC	Human Fab
PFBL-460	Human Anti-EGFR Recombinant Antibody (clone h-R3); Fab Fragment	ELISA, WB, FuncS	Humanized Fab
HPAB-0044-FY-F(E)	Human Anti-EGFR Recombinant Antibody (clone TGM10-1); Fab Fragment	FC	Humanized Fab
HPAB-0089-FY-F(E)	Human Anti-EGFR Recombinant Antibody (clone MIL27-3); Fab Fragment	FC	Humanized Fab
HPAB-0119-FY-F(E)	Human Anti-EGFR Recombinant Antibody (clone huML66); Fab Fragment	Inhib	Humanized Fab

CAT	Product Name	Application	Type
TAB-0225CL-S(P)	Human Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-0225CL-S(P))	Block, Inhib, FuncS, Apop, In vivo	Human scFv
TAB-272MZ	Human Anti-EGFR Recombinant Antibody (TAB-272MZ)	FuncS	Chimeric (mouse/human) IgG
TAB-272MZ-S(P)	Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-272MZ-S(P))	FuncS	Mouse scFv
TAB-302MZ-S(P)	Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-302MZ-S(P))	ELISA	Mouse scFv
TAB-302MZ-F(E)	Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-302MZ-F(E))	ELISA	Chimeric (mouse/human) Fab

CAT	Product Name	Application	Type
TAB-0564CL-S(P)	Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-0564CL-S(P))	ELISA	Mouse scFv
TAB-0565CL-S(P)	Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-0565CL-S(P))	ELISA	Mouse scFv
TAB-303MZ-F(E)	Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-303MZ-F(E))	ELISA	Mouse Fab
TAB-304MZ-F(E)	Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-304MZ-F(E))	ELISA	Mouse Fab
TAB-326MZ-F(E)	Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-326MZ-F(E))	FC	Mouse Fab

CAT	Product Name	Application	Type
TAB-274MZ	Human Anti-EGFR Recombinant Antibody (TAB-274MZ)	FC	Humanized IgG
TAB-297MZ	Anti-Human EGFR Recombinant Antibody (H225)	ELISA, WB	Humanized antibody
TAB-298MZ	Anti-Human EGFR Recombinant Antibody (Hu225)	ELISA, WB	Humanized antibody
TAB-274MZ-S(P)	Human Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-274MZ-S(P))	FC	Humanized scFv
TAB-274MZ-F(E)	Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-274MZ-F(E))	FC	Humanized Fab

CAT	Product Name	Application	Type
TAB-313MZ-F(E)	Anti-Human EGFR Recombinant Antibody Fab Fragment (EG-19-11)	ELISA, FC	Human antibody
TAB-314MZ-F(E)	Anti-Human EGFR Recombinant Antibody Fab Fragment (EG-26-11)	ELISA, FC	Human antibody
TAB-315MZ-F(E)	Anti-Human EGFR Recombinant Antibody Fab Fragment (528)	WB, FC	Human antibody
PABX-053	Recombinant Human Anti-EGFR Antibody (DL11)	WB, ELISA, FuncS	IgG
PABX-053-F (E)	Recombinant Human Anti-EGFR Antibody Fab Fragment (DL11)	WB, ELISA, FuncS	Fab

CAT	Product Name	Application	Type
Gly-055LC	Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated)	ELISA	Chimeric antibody (mouse/human)
Gly-144LC	Recombinant Anti-Human EGFR Antibody (Fc glycosylation)	ELISA	Humanized antibody

CAT	Product Name	Application	Type
Gly-055LC-1	Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated)	ELISA	Chimeric antibody (mouse/human)

CAT	Product Name	Application	Type
Gly-167LC	Recombinant Anti-Human EGFR Antibody (Non-glycosylated)	ELISA	Human antibody

CAT	Product Name	Application	Type
BRD-0183MZ	Chicken Anti-EGFR Polyclonal IgY	WB	Chicken antibody
BRD-0668MZ	Chicken Anti-EGFR Polyclonal IgY	WB	Chicken antibody

CAT	Product Name	Application
MHC-LC773	A*0201/Human EGFR (YLNTVQPTCV) MHC Tetramer	FCM
MHC-LC4545	PE-DQB1*03:02/Human EGFR (SRALEEKKGNYVVTHG) MHC Tetramer	FCM
MHC-YF409	A*02:01/Human EGF-R-479 (KLFGTSGQKT) MHC Pentamer	FCM

CAT	Product Name	Application	Type
NEUT-722CQ	Rabbit Anti-EGFR Recombinant Antibody (clone CBL1011)	Neut	Rabbit IgG
NEUT-723CQ	Mouse Anti-EGFR Recombinant Antibody (clone CBL931)	WB, IP, IHC, ICC, Neut	Mouse IgG1
NEUT-724CQ	Rabbit Anti-EGFR Recombinant Antibody (clone D1D4J)	IF, FC, WB, IP, Neut	Rabbit IgG

CAT	Product Name	Application	Type
MOR-1101	Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone DS1101AB)	IHC-P	Rabbit IgG
MOR-4520	Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH28DS)	IF, ICC, FC	Rabbit IgG
MOR-4675	Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH189DS)	WB, IF, ICC, FC	Rabbit IgG
MOR-4676	Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH190DS)	WB, IF, ICC, FC	Rabbit IgG
MOR-0033-FY	Rabbit Anti-EGFR Recombinant Antibody (clone AFY0004)	ICC, IHC, WB	Rabbit IgG

CAT	Product Name	Application	Type
FAMAB-0202-CN-S(P)	Human Anti-EGFR Recombinant Antibody (clone E10); scFv Fragment	ELISA, WB	Human scFv
HPAB-0419-FY-S(P)	Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (HPAB-0419-FY-S(P))	Cyt, ELISA, FC	Mouse scFv
HPAB-0420-FY-S(P)	Human Anti-EGFR Recombinant Antibody (clone MOR09823); scFv Fragment	Inhib	Human scFv
HPAB-2060-FY-S(P)	Mouse Anti-EGFR Recombinant Antibody (clone L3-11D); scFv Fragment	FC, ELISA	Mouse scFv
HPAB-2098-FY-S(P)	Mouse Anti-EGFR Recombinant Antibody (clone Mint5); scFv Fragment	ICC, Inhib	Mouse scFv

CAT	Product Name	Application	Type
AFC-TAB-165	Afuco™ Anti-EGFR ADCC Recombinant Antibody (Matuzumab), ADCC Enhanced	Neut, ELISA, IF, IP, FuncS, FC	ADCC enhanced antibody
AFC-TAB-464CQ	Afuco™ Anti-EGFR ADCC Recombinant Antibody (Tomuzotuximab), ADCC Enhanced	ELISA, IHC, FC, IP, IF, FuncS	ADCC enhanced antibody
AFC-TAB-003	Afuco™ Anti-EGFR ADCC Recombinant Antibody (Cetuximab), ADCC Enhanced	IF, IP, Neut, FuncS, ELISA, FC	ADCC enhanced antibody
AFC-TAB-040	Afuco™ Anti-EGFR ADCC Recombinant Antibody (Zalutumumab), ADCC Enhanced	ELISA, FC, IP, FuncS, IF, Neut	ADCC enhanced antibody
AFC-TAB-119	Afuco™ Anti-EGFR ADCC Recombinant Antibody (Necitumumab), ADCC Enhanced	FC, IP, ELISA, Neut, FuncS, IF	ADCC enhanced antibody

CAT	Product Name	Application	Type
HPAB-AP881-YC	Recombinant Llama Anti-EGFR Single Domain Antibody (VHH122)	ELISA, FC, IP, FuncS	Llama VHH
HPAB-AP882-YC	Recombinant Llama Anti-EGFR Single Domain Antibody (VHH205)	ELISA	Llama VHH
HPAB-AP883-YC	Recombinant Llama Anti-EGFR Single Domain Antibody (VHH03)	ELISA	Llama VHH
HPAB-AP884-YC	Recombinant Llama Anti-EGFR Single Domain Antibody (VHH139)	ELISA	Llama VHH
HPAB-AP885-YC	Recombinant Llama Anti-EGFR Single Domain Antibody (VHH107)	ELISA	Llama VHH

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

Anti-Human EGFR Recombinant Antibody (Necitumumab) (CAT#: TAB-119)

Subcellular Location and Protein Expression

Normal Tissue

RNA Expression

Specifications

Product Property

Target

Related Resources

Product Notes

Downloads

See other products for "Necitumumab"

See other products for "EGFR"

Recombinant Antibody

Fab Fragment Antibody

Chimeric Antibody

Mouse Antibody

Humanized Antibody

Human Antibody

Fc Glycosylation

High-mannose Glycoform

Deglycosylated Antibody (Non-glycosylated IgGs)

Chicken IgY Antibody

MHC Tetramer for Cancer

Neutralizing Antibody

Rabbit Monoclonal Antibody

scFv Fragment Antibody

ADCC Enhanced Antibody

Single-domain Antibody

Customer Reviews and Q&As