Anti-Human MS4A1 Recombinant Antibody (1F5) (CAT#: TAB-1632CL)

Figure 1 HB20-3,-4, and-25 monoclonal antibodies bind to cell surface CD20 at a higher density than known anti-CD20 monoclonal antibodies.

Reactivity of human blood lymphocytes (Figure A) and the Raji B lymphoblastoid cell line (Figure B) with 1 F5, HB20-3 and B1 anti-CD20 monoclonal antibodies (solid lines) or secondary antibody alone (dashed line) is shown. The anti-CD20 monoclonal antibodies were used at concentrations that were predetermined to be saturating and to give optimal staining: 1F5 as ascites fluid diluted 1:200; HB20-3 as tissue culture supernatant fluid from the HB20-3 hybridoma; or B1 monoclonal antibody at either 10 μg/mL of purified monoclonal antibody or as tissue culture supernatant fluid. In all cases, monoclonal antibody staining was visualized using PE-conjugated isotype-specific secondary antibodies with flow cytometry analysis. Results represent those obtained in>3 experiments.