Human Anti-NRP1 Recombinant Antibody (HPAB-0514-CN) (CAT#: HPAB-0514-CN)

This product is a recombinant human antibody that recognizes NRP1. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.

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WB

Figure 1 Human Anti-NRP1 Recombinant Antibody (HPAB-0514-CN) in WB

Figure 1 Human Anti-NRP1 Recombinant Antibody (HPAB-0514-CN) in WB

Western blot analysis of HPAB-0514-CN was performed with Recombinant Human NRP1 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with HPAB-0514-CN and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.

ELISA

Figure 2 Human Anti-NRP1 Recombinant Antibody (HPAB-0514-CN) in ELISA

Figure 2 Human Anti-NRP1 Recombinant Antibody (HPAB-0514-CN) in ELISA

ELISA analysis of HPAB-0514-CN was performed by coating with Recombinant Human NRP1 Protein. Then blocked with BSA, and incubated with Anti-NRP1 Recombinant Antibody (HPAB-0514-CN). The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

DB

Figure 3 Human Anti-NRP1 Recombinant Antibody (HPAB-0514-CN) in DB

Figure 3 Human Anti-NRP1 Recombinant Antibody (HPAB-0514-CN) in DB

Antigen: Human NRP1 protein
Antibody incubation concentration: 2ng/μL.


Specifications

  • Immunogen
  • CHO cell expressed hNRP1-Fc protein
  • Host Species
  • Human
  • Derivation
  • Human
  • Type
  • Human IgG1
  • Species Reactivity
  • Human, Mouse
  • Applications
  • ELISA, FC, Block
  • Related Disease
  • Disorders associated with pathological angiogenesis

Product Property

  • Purity
  • >95% as determined by SDS-PAGE
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • NP1; NRP; BDCA4; CD304; VEGF165R

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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