Human Anti-NRP1 Recombinant Antibody (HPAB-0514-CN) (CAT#: HPAB-0514-CN)
This product is a recombinant human antibody that recognizes NRP1. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.
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WB
Figure 1 Human Anti-NRP1 Recombinant Antibody (HPAB-0514-CN) in WB
Western blot analysis of HPAB-0514-CN was performed with Recombinant Human NRP1 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with HPAB-0514-CN and HRP Goat Anti-Human IgG as a secondary antibody. Chemiluminescent detection was performed.
ELISA
Figure 2 Human Anti-NRP1 Recombinant Antibody (HPAB-0514-CN) in ELISA
ELISA analysis of HPAB-0514-CN was performed by coating with Recombinant Human NRP1 Protein. Then blocked with BSA, and incubated with Anti-NRP1 Recombinant Antibody (HPAB-0514-CN). The HRP-conjugated goat anti-human IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
DB
Figure 3 Human Anti-NRP1 Recombinant Antibody (HPAB-0514-CN) in DB
Antigen: Human NRP1 protein
Antibody incubation concentration: 2ng/μL.
Specifications
- Immunogen
- CHO cell expressed hNRP1-Fc protein
- Host Species
- Human
- Derivation
- Human
- Type
- Human IgG1
- Species Reactivity
- Human, Mouse
- Applications
- ELISA, FC, Block
- Related Disease
- Disorders associated with pathological angiogenesis
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
Related Resources
Krasemann, Susanne, et al. "The blood-brain barrier is dysregulated in COVID-19 and serves as a CNS entry route for SARS-CoV-2." Stem cell reports 17.2 (2022): 307-320. https://doi.org/10.1016/j.stemcr.2021.12.011
This study investigates how SARS-CoV-2 affects the blood-brain barrier (BBB) and potentially enters the central nervous system. The researchers developed a human induced pluripotent stem cell (hiPSC)-derived brain capillary endothelial-like cell model to study SARS-CoV-2 infection mechanisms. They demonstrated that these cells can be infected by SARS-CoV-2, leading to active viral replication and transcellular transport across the BBB without compromising its paracellular tightness. Transcriptomic analysis of both COVID-19 patient brain vessels and infected hiPSC-derived cells showed significant upregulation of interferon signaling pathways. The researchers also found that viral entry could be reduced using antibodies against spike protein, ACE2, and neuropilin-1 (NRP1), as well as with the TMPRSS2 inhibitor nafamostat.
Creative Biolabs provided anti-NRP1 antibodies (HPAB-0514-CN) that were used in conjunction with other anti-NRP1 antibodies to test potential therapeutic interventions against SARS-CoV-2 infection of brain endothelial cells. These antibodies helped demonstrate that NRP1, which showed higher expression than ACE2 in the brain endothelial cells, plays a significant role in viral entry. The successful inhibition of infection using these antibodies suggests that targeting NRP1 could be a promising strategy for preventing SARS-CoV-2 neuroinvasion, potentially addressing the neurological complications observed in COVID-19 patients.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-264 | Anti-Human NRP1 Recombinant Antibody (Vesencumab) | IP, IF, FuncS, FC, Neut, ELISA, ICC | IgG1 - kappa |
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