Mouse Anti-BSG Recombinant Antibody (clone M6-1B9) (CAT#: VS-0323-CJ4)
This mouse anti-BSG antibody (clone M6-1B9) is a product that recognizes Human 293A cells CD147.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Inhib
Figure 1 Competitive binding analysis of soluble scFv-M6-1B9 and mAb M6-1B9.
A. untransduced; B. scFv-M6-1B9; C. scFv-SVV3 transduced cells.
FC
Figure 2 Inhibition of CD147 surface expression on 293A cells by M6-1B9 (VS-0323-CJ4).
ICC
Figure 3 Immunocytochemical analysis for colocalization of CD147-scFv-M6-1B9 (VS-0323-CJ4).
A. CD147 on transfected 293A cell stained with biotinylated anti-human EMMPRIN mAb (red), B. scFv-M6-1B9 (VS-0323-CJ4) in transfected 293A cell stained with rabbit anti-HA mAb (white), C. GFP positive in transfected 293A cell and D overlay.
ELISA
Figure 4 ELISA analysis of the binding of soluble scFv-M6-1B9 to CD147-BCCP (VS-0323-CJ4).
CD147-BCCP (open columns) or SVV-BCCP (black columns) was captured on the avidin-coated wells. Soluble scFv-M6-1B9 was subsequently added and the bound scFv was detected by peroxidase-conjugated mAb anti-HA.
WB
Figure 5 Western blot analysis of the binding of soluble scFv-M6-1B9 to CD147-BCCP (VS-0323-CJ4).
Anti-HA (Lane 1) and anti-His mAb (Lane 2).
Specifications
- Immunogen
- Recombinant CD147-BCCP
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human 293A cells CD147
- Species Reactivity
- Human
- Clone
- M6-1B9
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry, Immunocytochemistry, Inhibition, Immunofluorescence
- Conjugate
- Unconjugated
- Related Disease
- Tumor
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- ELISA: 1/10-1/1000
Inhibition assay: 0-10 μg/mL
Immunofluorescence: 1/20
Flow Cytometry: 10 μg/ml
Target
- Alternative Names
- OK; 5F7; TCSF; CD147; EMPRIN; HAb18G; EMMPRIN
- Gene ID
- 682
- UniProt ID
- P35613
- Cellular Localization
- Cell membrane; Cell projection; Endoplasmic reticulum; Endosome; Membrane
- Post Translation Modifications
- Isoform 2: N-glycosylated.
Isoform 3: N-glycosylated.
Isoform 4: N-glycosylated.
- Protein Refseq
- NP_001309172.1; NP_001719.2; NP_940991.1
- Function
- Isoform 1:
Essential for normal retinal maturation and development (By similarity).
Acts as a retinal cell surface receptor for NXNL1 and plays an important role in NXNL1-mediated survival of retinal cone photoreceptors (PubMed:25957687).
In association with glucose transporter SLC16A1/GLUT1 and NXNL1, promotes retinal cone survival by enhancing aerobic glycolysis and accelerating the entry of glucose into photoreceptors (PubMed:25957687).
May act as a potent stimulator of IL6 secretion in multiple cell lines that include monocytes (PubMed:21620857).
Isoform 2
Signaling receptor for cyclophilins, essential for PPIA/CYPA and PPIB/CYPB-dependent signaling related to chemotaxis and adhesion of immune cells (PubMed:11943775, PubMed:11688976).
Plays an important role in targeting monocarboxylate transporters SLC16A1/GLUT1, SLC16A11 and SLC16A12 to the plasma membrane (PubMed:17127621, PubMed:21778275, PubMed:28666119).
Acts as a coreceptor for vascular endothelial growth factor receptor 2 (KDR/VEGFR2) in endothelial cells enhancing its VEGFA-mediated activation and downstream signaling (PubMed:25825981).
Promotes angiogenesis through EPAS1/HIF2A-mediated up-regulation of VEGFA (isoform VEGF-165 and VEGF-121) and KDR/VEGFR2 in endothelial cells (PubMed:19837976).
Plays a key role in regulating tumor growth, invasion, metastasis and neoangiogenesis by stimulating the production and release of extracellular matrix metalloproteinases and KDR/VEGFR2 by both tumor cells and stromal cells (fibroblasts and endothelial cells) (PubMed:12553375, PubMed:11992541, PubMed:15833850).
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "BSG"
Select a product category from the dropdown menu below to view related products.
Please select product type
Intrabody Products
IgM Antibody Products
Human Antibody Products
IgG Antibody Products
Fab Antibody Products
ScFv Antibody Products
Blocking Antibody Products
Rabbit Monoclonal Antibody Products
Neuroscience Antibody Products
ADCC Enhanced Antibody Products
Antibody Magnetic Beads
ScFv-Fc Chimera Products
Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B050(A) | Recombinant Anti-human BSG Intrabody [(D-Arg)9] | WB, ICC, Neut, FuncS | scFv-(D-Arg)9 |
| IAB-B050(G) | Recombinant Anti-human BSG Intrabody [+36 GFP] | IF, FC, FuncS | scFv-(+36GFP) |
| IAB-B050(T) | Recombinant Anti-human BSG Intrabody [Tat] | WB, ICC, FuncS | scFv-Tat |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for VS-0323-CJ4. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: FuncS, IF, Neut, ELISA, FC, IP, WB
Application: WB, ELISA, FuncS
Application: ELISA, FuncS, IHC, IF, FC, ADCC
-3.jpg)
Application: Neut
Application: ELISA, WB, FC
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
