Mouse Anti-BSG Recombinant Antibody (clone M6-1B9)
CAT#: VS-0323-CJ4
This mouse anti-BSG antibody (clone M6-1B9) is a product that recognizes Human 293A cells CD147.
Gene Expression
Published Data
Subcellular Location
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/74870/1466_A2_3_selected.jpg
Normal Tissue
Figure 2 Testis
Sertoli cells Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2427/6189_A_4_6.jpg
Normal Tissue
Figure 3 Heart muscle
Cardiomyocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2427/6189_B_6_6.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2427/6189_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity: 75%-25% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2427/6189_A_7_3.jpg
Normal Tissue
Figure 6 Kidney
Collecting ducts Staining: Medium Intensity: Strong Quantity: <25% Distal tubules Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2427/6189_A_7_5.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000172270-BSG
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Inhib
Figure 1 Competitive binding analysis of soluble scFv-M6-1B9 and mAb M6-1B9.
A. untransduced; B. scFv-M6-1B9; C. scFv-SVV3 transduced cells.
FC
Figure 2 Inhibition of CD147 surface expression on 293A cells by M6-1B9 (VS-0323-CJ4).
ICC
Figure 3 Immunocytochemical analysis for colocalization of CD147-scFv-M6-1B9 (VS-0323-CJ4).
A. CD147 on transfected 293A cell stained with biotinylated anti-human EMMPRIN mAb (red), B. scFv-M6-1B9 (VS-0323-CJ4) in transfected 293A cell stained with rabbit anti-HA mAb (white), C. GFP positive in transfected 293A cell and D overlay.
ELISA
Figure 4 ELISA analysis of the binding of soluble scFv-M6-1B9 to CD147-BCCP (VS-0323-CJ4).
CD147-BCCP (open columns) or SVV-BCCP (black columns) was captured on the avidin-coated wells. Soluble scFv-M6-1B9 was subsequently added and the bound scFv was detected by peroxidase-conjugated mAb anti-HA.
WB
Figure 5 Western blot analysis of the binding of soluble scFv-M6-1B9 to CD147-BCCP (VS-0323-CJ4).
Anti-HA (Lane 1) and anti-His mAb (Lane 2).
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Specifications
- Immunogen
- Recombinant CD147-BCCP
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human 293A cells CD147
- Species Reactivity
- Human
- Clone
- M6-1B9
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry, Immunocytochemistry, Inhibition, Immunofluorescence
- Conjugate
- Unconjugated
- Related Disease
- Tumor
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- ELISA: 1/10-1/1000
Inhibition assay: 0-10 μg/mL
Immunofluorescence: 1/20
Flow Cytometry: 10 μg/ml
Target
- Alternative Names
- OK; 5F7; TCSF; CD147; EMPRIN; HAb18G; EMMPRIN
- Gene ID
- 682
- UniProt ID
- P35613
- Cellular Localization
- Cell membrane; Cell projection; Endoplasmic reticulum; Endosome; Membrane
- Post Translation Modifications
- Isoform 2: N-glycosylated.
Isoform 3: N-glycosylated.
Isoform 4: N-glycosylated.
- Protein Refseq
- NP_001309172.1; NP_001719.2; NP_940991.1
- Function
- Isoform 1:
Essential for normal retinal maturation and development (By similarity).
Acts as a retinal cell surface receptor for NXNL1 and plays an important role in NXNL1-mediated survival of retinal cone photoreceptors (PubMed:25957687).
In association with glucose transporter SLC16A1/GLUT1 and NXNL1, promotes retinal cone survival by enhancing aerobic glycolysis and accelerating the entry of glucose into photoreceptors (PubMed:25957687).
May act as a potent stimulator of IL6 secretion in multiple cell lines that include monocytes (PubMed:21620857).
Isoform 2
Signaling receptor for cyclophilins, essential for PPIA/CYPA and PPIB/CYPB-dependent signaling related to chemotaxis and adhesion of immune cells (PubMed:11943775, PubMed:11688976).
Plays an important role in targeting monocarboxylate transporters SLC16A1/GLUT1, SLC16A11 and SLC16A12 to the plasma membrane (PubMed:17127621, PubMed:21778275, PubMed:28666119).
Acts as a coreceptor for vascular endothelial growth factor receptor 2 (KDR/VEGFR2) in endothelial cells enhancing its VEGFA-mediated activation and downstream signaling (PubMed:25825981).
Promotes angiogenesis through EPAS1/HIF2A-mediated up-regulation of VEGFA (isoform VEGF-165 and VEGF-121) and KDR/VEGFR2 in endothelial cells (PubMed:19837976).
Plays a key role in regulating tumor growth, invasion, metastasis and neoangiogenesis by stimulating the production and release of extracellular matrix metalloproteinases and KDR/VEGFR2 by both tumor cells and stromal cells (fibroblasts and endothelial cells) (PubMed:12553375, PubMed:11992541, PubMed:15833850).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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