Mouse Anti-Cd28 Recombinant Antibody (clone JJ-316) (CAT#: HPAB-0078-YJ)

Figure 1 Blood samples from rats i.v. injected with 1.0 mg, 0.2 mg, or 0.04 mg JJ316 were taken at the indicated time points and analyzed for the percentages of circulating T cells (left panel) and granulocytes (right panel) by flow cytometry.

n = 6; mean ± standard error of the mean. Statistical analysis refers to the individual comparison of each time course to untreated control rats by 1-way ANOVA.

Müller, N., van den Brandt, J., Odoardi, F., Tischner, D., Herath, J., Flügel, A., & Reichardt, H. M. (2008). A CD28 superagonistic antibody elicits 2 functionally distinct waves of T cell activation in rats. The Journal of clinical investigation, 118(4), 1405-1416.

Figure 2 Leukocytes were isolated from lymph nodes, spleen, lung, and liver 10 and 72 hours after injection of 1.0 mg JJ316 or PBS as a control.

Subsequently, the total number CD4+ T cells was determined by microscopic counting followed by flow cytometric analysis of CD4 and TCRβ expression. n = 3; mean ± standard error of the mean.

Müller, N., van den Brandt, J., Odoardi, F., Tischner, D., Herath, J., Flügel, A., & Reichardt, H. M. (2008). A CD28 superagonistic antibody elicits 2 functionally distinct waves of T cell activation in rats. The Journal of clinical investigation, 118(4), 1405-1416.

Figure 3 The relative frequencies of CD4+ naive (CD45RC+RT6.1+), activated (CD45RC-RT6.1-), and memory T cells (CD45RC-RT6.1+/CD45RC+RT6.1-) was determined 10 and 72 hours after application of JJ316 or PBS.

Thy-1+ RTEs (recent thymic emigrants) were excluded from the analysis. n = 3; mean ± standard error of the mean.

Müller, N., van den Brandt, J., Odoardi, F., Tischner, D., Herath, J., Flügel, A., & Reichardt, H. M. (2008). A CD28 superagonistic antibody elicits 2 functionally distinct waves of T cell activation in rats. The Journal of clinical investigation, 118(4), 1405-1416.

Figure 4 CFSE-labeled lymphocytes (4 × 10⁷) were transferred into recipient rats i.v., and 3 days later 0.2 mg JJ316 or PBS were administered.

After 10 hours the frequencies of CFSE+CD4+ T cells in blood, liver, lung, and spleen were determined by microscopic counting and flow cytometry. n = 3; mean ± standard error of the mean. *P < 0.05; **P < 0.01; ***P < 0.001.

Müller, N., van den Brandt, J., Odoardi, F., Tischner, D., Herath, J., Flügel, A., & Reichardt, H. M. (2008). A CD28 superagonistic antibody elicits 2 functionally distinct waves of T cell activation in rats. The Journal of clinical investigation, 118(4), 1405-1416.