Human Anti-EGFR Recombinant Antibody (clone E6.3); scFv Fragment (CAT#: HPAB-S0021-YC-S(P))
There is provided a fully human monoclonal antibody against human epidermal growth factor receptor (EGF-r) that possess similar or enhanced activities as compared to antibody C225 in order to vitiate concerns and/or effects of HAMA or HACA response. The antibody secreted by the hybridoma E6.3 comprises a human IgG2 antibody having a human kappa light chain.
Host: Human
Isotype: Human scFv
Applications: ELISA, Inhib
Specific Inquiry
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cell junctions.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/144_E9_2_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cell junctions.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/144_E9_2_selected.jpg
Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/143_E9_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/143_E9_1_blue_red_green.jpg
Normal Tissue
Figure 3 Colon
(Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_3.jpg
(Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_3.jpg
Figure 4 Liver
(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_4.jpg
(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_4.jpg
Figure 5 Kidney
(Bowman's capsule
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Collecting ducts
Staining:Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Proximal tubules (cell body)
Staining:Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_5.jpg
(Bowman's capsule
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Collecting ducts
Staining:Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Proximal tubules (cell body)
Staining:Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_5.jpg
Figure 6 Testis
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_6_6.jpg
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_6_6.jpg
Figure 7 Placenta
(Cytotrophoblasts
Staining:High
Intensity: Strong
Quantity:>75%
Decidual cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Hofbauer cells
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - cell body
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_1_7.jpg
(Cytotrophoblasts
Staining:High
Intensity: Strong
Quantity:>75%
Decidual cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Hofbauer cells
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - cell body
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_1_7.jpg
Figure 8 Lymph node
(Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_8.jpg
(Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enriched (A-431)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000146648-EGFR
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000146648-EGFR
Specifications
- Host Species
- Human
- Type
- Human scFv
- Specificity
- Human EGFR
- Species Reactivity
- Human
- Clone
- E6.3
- Applications
- ELISA, Inhib
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Epidermal Growth Factor Receptor; Receptor Tyrosine-Protein Kinase ErbB-1; Erb-B2 Receptor Tyrosine Kinase 1; Proto-Oncogene C-ErbB-1; EC 2.7.10.1; ERBB1; ERBB; HER1; Epidermal Growth Factor Receptor (Avian Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog); Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog (Avian)
- Gene ID
- 1956
- UniProt ID
- P00533
Related Resources
Bladder Cancer
Non-small Cell Lung Cancer
Pancreatic Cancer
Hepatocellular Carcinoma
Cushing Syndrome
JAK-STAT Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone E6.3"
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Single-domain Antibody
| CAT | Product Name | Application | Type |
| NABG-056 | Recombinant Anti-Mouse Egfr VHH Single Domain Antibody | ELISA, IHC, FC, FuncS | Llama VHH |
| PNBL-016 | Recombinant Anti-Human EGFR VHH Single Domain Antibody (7D12) | WB, ELISA | Llama VHH |
| PNBL-017 | Recombinant Anti-Human EGFR VHH Single Domain Antibody (9G8) | FuncS, ELISA, IF | Llama VHH |
| PNBL-018 | Recombinant Anti-Human EGFR VHH Single Domain Antibody (EgA1) | FuncS, SPR | Llama VHH |
| HPAB-AP881-YC | Recombinant Llama Anti-EGFR Single Domain Antibody (VHH122) | ELISA, FC, IP, FuncS | Llama VHH |
Chimeric Antibody
| CAT | Product Name | Application | Type |
| TAB-H35 | Anti-Human EGFR Recombinant Antibody (Futuximab) | IF, WB, Inhib | IgG1 - kappa |
| TAB-0225CL-S(P) | Human Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-0225CL-S(P)) | Block, Inhib, FuncS, Apop, In vivo | Human scFv |
| TAB-0225CL-F(E) | Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-0225CL-F(E)) | Block, Inhib, FuncS, Apop, In vivo | Chimeric (Mouse/Human) Fab |
| TAB-272MZ | Human Anti-EGFR Recombinant Antibody (TAB-272MZ) | FuncS | Chimeric (mouse/human) IgG |
| TAB-302MZ | Human Anti-EGFR Recombinant Antibody (TAB-302MZ) | ELISA | Chimeric (mouse/human) IgG1 |
Human Antibody
| CAT | Product Name | Application | Type |
| TAB-040 | Anti-Human EGFR Recombinant Antibody (Zalutumumab) | ELISA, FC, IP, FuncS, IF, Neut, ICC | IgG1 - kappa |
| TAB-119 | Anti-Human EGFR Recombinant Antibody (Necitumumab) | FC, IP, ELISA, Neut, FuncS, IF, WB | IgG1 - kappa |
| TAB-313MZ-F(E) | Anti-Human EGFR Recombinant Antibody Fab Fragment (EG-19-11) | ELISA, FC | Human antibody |
| PABX-053 | Recombinant Human Anti-EGFR Antibody (DL11) | WB, ELISA, FuncS | IgG |
| PABX-053-F (E) | Recombinant Human Anti-EGFR Antibody Fab Fragment (DL11) | WB, ELISA, FuncS | Fab |
Recombinant Antibody
| CAT | Product Name | Application | Type |
| PABW-088 | Mouse Anti-EGFR Recombinant Antibody (clone 7A7) | ELISA, WB | Mouse IgG |
| PSBW-088 | Mouse Anti-EGFR Recombinant Antibody (clone 7A7); scFv Fragment | ELISA, WB | Mouse scFv |
| FAMAB-0094YC | Human Anti-EGFR Recombinant Antibody (clone F4) | ELISA, WB, FC | Human IgG |
| HPAB-0044-FY | Human Anti-EGFR Recombinant Antibody (clone TGM10-1) | FC | Humanized IgG |
| HPAB-0089-FY | Human Anti-EGFR Recombinant Antibody (clone MIL27-3) | FC | Human IgG |
Humanized Antibody
| CAT | Product Name | Application | Type |
| TAB-274MZ | Human Anti-EGFR Recombinant Antibody (TAB-274MZ) | FC | Humanized IgG |
| TAB-300MZ | Human Anti-EGFR Recombinant Antibody (TAB-300MZ) | ELISA | Humanized IgG |
| TAB-274MZ-S(P) | Human Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-274MZ-S(P)) | FC | Humanized scFv |
| TAB-274MZ-F(E) | Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-274MZ-F(E)) | FC | Humanized Fab |
| PABX-052 | Recombinant Human Anti-EGFR Antibody (Nimotuzumab) | WB, ELISA, FuncS, IB, FC, SPR, Apop | IgG |
Mouse Antibody
| CAT | Product Name | Application | Type |
| TAB-310MZ-F(E) | Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-310MZ-F(E)) | ELISA | Mouse Fab |
| TAB-311MZ-F(E) | Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-311MZ-F(E)) | ELISA | Mouse Fab |
| TAB-312MZ-F(E) | Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-312MZ-F(E)) | ELISA | Mouse Fab |
| TAB-316MZ-F(E) | Anti-Human EGFR Recombinant Antibody Fab Fragment (07D06) | WB, MTT assay | |
| TAB-317MZ-F(E) | Anti-Human EGFR Recombinant Antibody Fab Fragment (12D03) | WB, MTT assay |
Fc Glycosylation
| CAT | Product Name | Application | Type |
| Gly-055LC | Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated) | ELISA | Chimeric antibody (mouse/human) |
| Gly-144LC | Recombinant Anti-Human EGFR Antibody (Fc glycosylation) | ELISA | Humanized antibody |
High-mannose Glycoform
| CAT | Product Name | Application | Type |
| Gly-055LC-1 | Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated) | ELISA | Chimeric antibody (mouse/human) |
Deglycosylated Antibody (Non-glycosylated IgGs)
| CAT | Product Name | Application | Type |
| Gly-167LC | Recombinant Anti-Human EGFR Antibody (Non-glycosylated) | ELISA | Human antibody |
Chicken IgY Antibody
| CAT | Product Name | Application | Type |
| BRD-0183MZ | Chicken Anti-EGFR Polyclonal IgY | WB | Chicken antibody |
| BRD-0668MZ | Chicken Anti-EGFR Polyclonal IgY | WB | Chicken antibody |
MHC Tetramer for Cancer
| CAT | Product Name | Application | Type |
| MHC-LC773 | A*0201/Human EGFR (YLNTVQPTCV) MHC Tetramer | FCM | |
| MHC-LC4545 | PE-DQB1*03:02/Human EGFR (SRALEEKKGNYVVTHG) MHC Tetramer | FCM | |
| MHC-YF409 | A*02:01/Human EGF-R-479 (KLFGTSGQKT) MHC Pentamer | FCM |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
| NEUT-722CQ | Rabbit Anti-EGFR Recombinant Antibody (clone CBL1011) | Neut | Rabbit IgG |
| NEUT-723CQ | Mouse Anti-EGFR Recombinant Antibody (clone CBL931) | WB, IP, IHC, ICC, Neut | Mouse IgG1 |
| NEUT-724CQ | Rabbit Anti-EGFR Recombinant Antibody (clone D1D4J) | IF, FC, WB, IP, Neut | Rabbit IgG |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
| MOR-1101 | Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone DS1101AB) | IHC-P | Rabbit IgG |
| MOR-4520 | Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH28DS) | IF, ICC, FC | Rabbit IgG |
| MOR-4675 | Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH189DS) | WB, IF, ICC, FC | Rabbit IgG |
| MOR-4676 | Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH190DS) | WB, IF, ICC, FC | Rabbit IgG |
| MOR-0033-FY | Rabbit Anti-EGFR Recombinant Antibody (clone AFY0004) | ICC, IHC, WB | Rabbit IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
| HPAB-0010-YC-F(E) | Mouse Anti-EGFR Recombinant Antibody (clone L211C); Fab Fragment | ELISA, FC | Mouse Fab |
| HPAB-0011-YC-F(E) | Human Anti-EGFR Recombinant Antibody (clone Pep 1); Fab Fragment | FC | Human Fab |
| HPAB-0012-YC-F(E) | Human Anti-EGFR Recombinant Antibody (clone Pep 2); Fab Fragment | FC | Human Fab |
| HPAB-0013-YC-F(E) | Human Anti-EGFR Recombinant Antibody (clone Pep 3); Fab Fragment | FC | Human Fab |
| HPAB-0014-YC-F(E) | Human Anti-EGFR Recombinant Antibody (clone Pep 4); Fab Fragment | FC | Human Fab |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
| AFC-TAB-165 | Afuco™ Anti-EGFR ADCC Recombinant Antibody (Matuzumab), ADCC Enhanced | Neut, ELISA, IF, IP, FuncS, FC | ADCC enhanced antibody |
| AFC-TAB-464CQ | Afuco™ Anti-EGFR ADCC Recombinant Antibody (Tomuzotuximab), ADCC Enhanced | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
| AFC-TAB-003 | Afuco™ Anti-EGFR ADCC Recombinant Antibody (Cetuximab), ADCC Enhanced | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
| AFC-TAB-040 | Afuco™ Anti-EGFR ADCC Recombinant Antibody (Zalutumumab), ADCC Enhanced | ELISA, FC, IP, FuncS, IF, Neut | ADCC enhanced antibody |
| AFC-TAB-119 | Afuco™ Anti-EGFR ADCC Recombinant Antibody (Necitumumab), ADCC Enhanced | FC, IP, ELISA, Neut, FuncS, IF | ADCC enhanced antibody |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
| HPAB-0419-FY-S(P) | Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (HPAB-0419-FY-S(P)) | Cyt, ELISA, FC | Mouse scFv |
| HPAB-2060-FY-S(P) | Mouse Anti-EGFR Recombinant Antibody (clone L3-11D); scFv Fragment | FC, ELISA | Mouse scFv |
| HPAB-1530WJ-S(P) | Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (clone 340) | ELISA, WB | Mouse scFv |
| HPAB-1531WJ-S(P) | Human Anti-EGFR Recombinant Antibody; scFv Fragment (clone hu340) | ELISA, WB | Human scFv |
| HPAB-1563WJ-S(P) | Human Anti-EGFR Recombinant Antibody; scFv Fragment (clone chMint5) | ELISA, WB | Human scFv |
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* Abbreviations
AgonistAgonist
BABioassay
InhibInhibition
PGProteogenomics
StimStimulation
WBWestern Blot
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