Human Anti-EGFR Recombinant Antibody (clone 2F8) (CAT#: HPAB-0526-WJ)
This product is a human antibody specifically binds to human EGFR. This antibody can be used for treating and preventing diseases related to expression of EGFR, particularly EGFR- expressing tumors and autoimmune diseases.
Specific Inquiry
IF
Figure 1 EGFR-mediated internalization of 2F8 by confocal microscopy.
A431 cells were incubated for 1 hour with 2F8-FITC (green). cell membranes were stained with WGA-rhodamine (red). M and N, membranes and nuclei, respectively. EGFR located intracellularly in lysosome-like vesicles (white arrow).
van Bueren, J. J. L., Bleeker, W. K., Bøgh, H. O., Houtkamp, M., Schuurman, J., van de Winkel, J. G., & Parren, P. W. (2006). Effect of target dynamics on pharmacokinetics of a novel therapeutic antibody against the epidermal growth factor receptor: implications for the mechanisms of action. Cancer research, 66(15), 7630-7638.
IHC
Figure 2 Tissue saturation and distribution in monkeys.
Tissue biopsies of the pharynxwere immunohistochemically stained for 2F8 using sheep anti-human IgG monkey preabsorbed antibodies (red-brown staining).
van Bueren, J. J. L., Bleeker, W. K., Bøgh, H. O., Houtkamp, M., Schuurman, J., van de Winkel, J. G., & Parren, P. W. (2006). Effect of target dynamics on pharmacokinetics of a novel therapeutic antibody against the epidermal growth factor receptor: implications for the mechanisms of action. Cancer research, 66(15), 7630-7638.
Specifications
- Host Species
- Human
- Derivation
- Humanized
- Type
- Humanized IgG
- Specificity
- Human EGFR
- Species Reactivity
- Human
- Clone
- 2F8
- Applications
- ELISA
- Related Disease
- Autoimmune disease
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Immunofluorescence and Immunohistochemistry. It's recommended that the optimal antibody concentration, dilution, incubition time, etc. be carefully titrated in specific assays.
Target
- Alternative Names
- EGFR; epidermal growth factor receptor; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
- Gene ID
- 1956
- UniProt ID
- P00533
Related Resources
Bladder Cancer
Non-small Cell Lung Cancer
Pancreatic Cancer
Hepatocellular Carcinoma
Cushing Syndrome
JAK-STAT Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone 2F8"
See other products for "EGFR"
Select a product category from the dropdown menu below to view related products.
Please select product type
Recombinant Antibody
Single-domain Antibody
Chimeric Antibody
Human Antibody
Humanized Antibody
Fab Fragment Antibody
Mouse Antibody
Fc Glycosylation
High-mannose Glycoform
Deglycosylated Antibody (Non-glycosylated IgGs)
Chicken IgY Antibody
MHC Tetramer for Cancer
Neutralizing Antibody
Rabbit Monoclonal Antibody
ADCC Enhanced Antibody
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1078z | Mouse Anti-EGFR Recombinant Antibody (clone 42C11) | WB, ELISA, FC, IF, IHC, FuncS | Mouse IgG1, κ |
| PABL-080 | Human Anti-EGFR Recombinant Antibody (PABL-080) | ELISA, WB, FuncS | Human IgG |
| PSBW-088 | Mouse Anti-EGFR Recombinant Antibody (clone 7A7); scFv Fragment | ELISA, WB | Mouse scFv |
| TAB-477CQ | Human Anti-EGFR Recombinant Antibody (clone Laprituximab) | ELISA, IHC, FC, IP, IF, WB | Chimeric (mouse/human) IgG1, κ |
| MOB-052LC | Mouse Anti-EGFR Recombinant Antibody (clone 7H7) | WB, IP | Mouse IgG |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for HPAB-0526-WJ. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: FuncS, IF, Neut, ELISA, FC, IP, IHC
Application: FC, IP, ELISA, Neut, FuncS, IF, ICC
Application: FC, IP, ELISA, Neut, FuncS, IF, WB
Application: ELISA, FC, IP, FuncS, IF, Neut, WB
Application: ELISA
Application: WB, ELISA, FC, IHC, IP
Application: ELISA
Application: ELISA, IHC, FC, IP, IF, Inhib
Application: ELISA, FC, Neut, Inhib
-1.png)
Application: ELISA
Application: ELISA, FC, FuncS
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
