Mouse Anti-EGFR Recombinant Antibody (VS3-CJ207)
CAT#: VS3-CJ207
This product is a mouse antibody that recognizes EGFR.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cell junctions.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/144_E9_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/143_E9_1_blue_red_green.jpg
Normal Tissue
Figure 3 Colon
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Bowman's capsule
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Collecting ducts
Staining:Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Proximal tubules (cell body)
Staining:Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_5.jpg
Normal Tissue
Figure 6 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_6_6.jpg
Normal Tissue
Figure 7 Placenta
Cytotrophoblasts
Staining:High
Intensity: Strong
Quantity:>75%
Decidual cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Hofbauer cells
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - cell body
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_1_7.jpg
Normal Tissue
Figure 8 Lymph node
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enriched (A-431)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000146648-EGFR
❮
❯
Specifications
- Immunogen
- Synthetic peptide
- Host Species
- Mouse
- Specificity
- Human EGFR
- Species Reactivity
- Human
- Applications
- WB
- Conjugate
- Unconjugated
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50% Glycerol, PBS, pH7.4.
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in Western Blot.
Target
- Alternative Names
- ERBB; ERBB1; HER1; PIG61; NISBD2; mENA; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1
- Gene ID
- 1956
- UniProt ID
- P00533
- Sequence Similarities
- Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
- Cellular Localization
- Cell membrane, Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane, Nucleus, Secreted
- Post Translation Modifications
- Phosphorylated on Tyr residues in response to EGF (PubMed:20462955, PubMed:27153536).
Phosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3. Dephosphorylated by PTPN1 and PTPN2.
Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting (PubMed:27153536).
Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occurs. Deubiquitination by OTUD7B prevents degradation. Ubiquitinated by RNF115 and RNF126 (By similarity).
Palmitoylated on Cys residues by ZDHHC20. Palmitoylation inhibits internalization after ligand binding, and increases the persistence of tyrosine-phosphorylated EGFR at the cell membrane. Palmitoylation increases the amplitude and duration of EGFR signaling.
Methylated. Methylation at Arg-1199 by PRMT5 stimulates phosphorylation at Tyr-1197.
- Protein Refseq
- NP_005219.2; NP_958439.1; NP_958440.1
- Function
- Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses (PubMed:2790960, PubMed:10805725, PubMed:27153536).
Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF (PubMed:2790960, PubMed:7679104, PubMed:8144591, PubMed:9419975, PubMed:15611079, PubMed:12297049, PubMed:27153536, PubMed:20837704, PubMed:17909029).
Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules (PubMed:27153536).
May also activate the NF-kappa-B signaling cascade (PubMed:11116146).
Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling (PubMed:11602604).
Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin (PubMed:11483589).
Positively regulates cell migration via interaction with CCDC88A/GIV which retains EGFR at the cell membrane following ligand stimulation, promoting EGFR signaling which triggers cell migration (PubMed:20462955).
Plays a role in enhancing learning and memory performance (By similarity).
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Related Diseases
Bladder Cancer
Non-small Cell Lung Cancer
Pancreatic Cancer
Hepatocellular Carcinoma
Cushing Syndrome
Related Signaling Pathways
JAK-STAT Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Recommended Dilution Buffer
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
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| TAB-272MZ-S(P) | Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-272MZ-S(P)) | FuncS | Mouse scFv |
| TAB-302MZ-F(E) | Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-302MZ-F(E)) | ELISA | Chimeric (mouse/human) Fab |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| TAB-477CQ | Human Anti-EGFR Recombinant Antibody (clone Laprituximab) | ELISA, IHC, FC, IP, IF, WB | Chimeric (mouse/human) IgG1, κ |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-301MZ | Mouse Anti-EGFR Recombinant Antibody (TAB-301MZ) | ELISA | Mouse IgG |
| TAB-303MZ | Mouse Anti-EGFR Recombinant Antibody (TAB-303MZ) | FC, FuncS, Inhib | Mouse IgG |
| TAB-304MZ | Mouse Anti-EGFR Recombinant Antibody (TAB-304MZ) | FC, FuncS, Inhib | Mouse IgG |
| TAB-305MZ | Mouse Anti-EGFR Recombinant Antibody (TAB-305MZ) | FC, FuncS, Inhib | Mouse IgG |
| TAB-306MZ | Mouse Anti-EGFR Recombinant Antibody (TAB-306MZ) | FC, FuncS, Inhib | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-278MZ-F(E) | Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-278MZ-F(E)) | ELISA, WB | Human Fab |
| TAB-279MZ-F(E) | Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-279MZ-F(E)) | ELISA, WB | Human Fab |
| TAB-282MZ-F(E) | Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-282MZ-F(E)) | ELISA | Human Fab |
| TAB-283MZ-F(E) | Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-283MZ-F(E)) | ELISA | Human Fab |
| TAB-284MZ-F(E) | Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-284MZ-F(E)) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-055LC | Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated) | ELISA | Chimeric antibody (mouse/human) |
| Gly-144LC | Recombinant Anti-Human EGFR Antibody (Fc glycosylation) | ELISA | Humanized antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-055LC-1 | Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated) | ELISA | Chimeric antibody (mouse/human) |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-167LC | Recombinant Anti-Human EGFR Antibody (Non-glycosylated) | ELISA | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0183MZ | Chicken Anti-EGFR Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC773 | A*0201/Human EGFR (YLNTVQPTCV) MHC Tetramer | FCM | |
| MHC-LC4545 | PE-DQB1*03:02/Human EGFR (SRALEEKKGNYVVTHG) MHC Tetramer | FCM | |
| MHC-YF409 | A*02:01/Human EGF-R-479 (KLFGTSGQKT) MHC Pentamer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-722CQ | Rabbit Anti-EGFR Recombinant Antibody (clone CBL1011) | Neut | Rabbit IgG |
| NEUT-723CQ | Mouse Anti-EGFR Recombinant Antibody (clone CBL931) | WB, IP, IHC, ICC, Neut | Mouse IgG1 |
| NEUT-724CQ | Rabbit Anti-EGFR Recombinant Antibody (NEUT-724CQ) | IF, FC, WB, IP, Neut | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1101 | Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone DS1101AB) | IHC-P | Rabbit IgG |
| MOR-4570 | Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH82DS) | ELISA | Rabbit IgG |
| MOR-4571 | Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH83DS) | WB, IF, ICC, FC | Rabbit IgG |
| MOR-4675 | Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH189DS) | WB, IF, ICC, FC | Rabbit IgG |
| MOR-4676 | Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH190DS) | WB, IF, ICC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0010-YC-S(P) | Mouse Anti-EGFR Recombinant Antibody (clone L211C); scFv Fragment | ELISA, FC | Mouse scFv |
| HPAB-0011-YC-S(P) | Human Anti-EGFR Recombinant Antibody (clone Pep 1); scFv Fragment | FC | Human scFv |
| HPAB-0012-YC-S(P) | Human Anti-EGFR Recombinant Antibody (clone Pep 2); scFv Fragment | FC | Human scFv |
| HPAB-0013-YC-S(P) | Human Anti-EGFR Recombinant Antibody (clone Pep 3); scFv Fragment | FC | Human scFv |
| HPAB-0014-YC-S(P) | Human Anti-EGFR Recombinant Antibody (clone Pep 4); scFv Fragment | FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0094-YC-F(E) | Human Anti-EGFR Recombinant Antibody (clone F4); Fab Fragment | ELISA, WB, FC | Human Fab |
| FAMAB-0202-CN-F(E) | Human Anti-EGFR Recombinant Antibody (clone E10); Fab Fragment | ELISA, WB | Human Fab |
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| HPAB-0089-FY-F(E) | Human Anti-EGFR Recombinant Antibody (clone MIL27-3); Fab Fragment | FC | Humanized Fab |
| HPAB-0119-FY-F(E) | Human Anti-EGFR Recombinant Antibody (clone huML66); Fab Fragment | Inhib | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| AFC-TAB-119 | Afuco™ Anti-EGFR ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-119) | FC, IP, ELISA, Neut, FuncS, IF | ADCC enhanced antibody |
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| AFC-TAB-753 | Afuco™ Anti-EGFR ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-753) | Neut, ELISA, IF, IP, FuncS, FC | ADCC enhanced antibody |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-AP881-YC | Recombinant Llama Anti-EGFR Single Domain Antibody (HPAB-AP881-YC) | ELISA, FC, IP, FuncS | Llama VHH |
| HPAB-AP882-YC | Recombinant Llama Anti-EGFR Single Domain Antibody (HPAB-AP882-YC) | ELISA | Llama VHH |
| HPAB-AP883-YC | Recombinant Llama Anti-EGFR Single Domain Antibody (HPAB-AP883-YC) | ELISA | Llama VHH |
| HPAB-AP884-YC | Recombinant Llama Anti-EGFR Single Domain Antibody (HPAB-AP884-YC) | ELISA | Llama VHH |
| HPAB-AP885-YC | Recombinant Llama Anti-EGFR Single Domain Antibody (HPAB-AP885-YC) | ELISA | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY84 | AbPlus™ Anti-EGFR Magnetic Beads (pSEX81-6) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC32 | Mouse Anti-EGFR Recombinant Antibody (VS-0924-YC32) - Cancer Stem Cell Marker | IHC, WB | Mouse IgG1 |
| VS-0924-YC35 | Rabbit Anti-EGFR Antibody (VS-0924-YC35) - Cancer Stem Cell Marker | IHC, WB, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY177 | Mouse Anti-NHP EGFR Recombinant Antibody (clone 225) | IF, IP | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0125-FY28 | Human Anti-EGFR (clone ABT-806) scFv-Fc Chimera | FC, Cyt | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY102 | CytoStream™ Mouse Anti-EGFR Recombinant Antibody (VS-0225-XY102) | FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY735 | Anti-EGFR Immunohistochemistry Kit | IHC | |
| VS-0525-XY2183 | Anti-Mouse EGFR Immunohistochemistry Kit | IHC | |
| VS-0525-XY2182 | Anti-Human EGFR Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC340 | Recombinant Anti-EGFR Vesicular Antibody, EV Displayed (VS-0425-YC340) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC65 | Recombinant Anti-EGFR (AA 269-278 x AA 526-535) Biparatopic Antibody, Tandem scFv (Clone Pep 2 x Clone Pep 3) | FC | Tandem scFv |
| VS-0525-YC66 | Recombinant Anti-EGFR (AA 582-591 x AA 606-614) Biparatopic Antibody, Tandem scFv (Clone Pep 4 x Clone Pep 1) | FC | Tandem scFv |
| VS-0525-YC68 | Recombinant Anti-EGFR (AA 526-535 x AA 600-605) Biparatopic Antibody, Tandem scFv (Clone Pep 3 x Clone Pep 5) | FC | Tandem scFv |
| VS-0525-YC213 | Recombinant Anti-EGFR (Domain II x Domain III) Biparatopic Antibody, Tandem scFv | ELISA, FC, IF, IHC, IP | Tandem scFv |
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