Mouse Anti-ITGB3 Recombinant Antibody (clone EM00212) (CAT#: FAMAB-1195CQ)

This product is a recombinant mouse antibody that recognizes ITGB3. The antibody was purified by affinity chromatography. It can be used in IHC.


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ELISA

Figure 1 ELISA profile of EBNA-recombinant rabbit anti-integrin monoclonal antibodies.

Figure 1 ELISA profile of EBNA-recombinant rabbit anti-integrin monoclonal antibodies.

Plates coated with soluble recombinant integrins (A) αvβ3; (B) αvβ5; (C) αvβ6; (D) αvβ8; or (E) native platelet gpiibβ3 (1 µg/ml) were incubated with recombinant antibodies from clones EM22703 (circles, closed); EM09902 (squares); EM05201 (triangles, up); EM13309 (diamonds); EM01309 (triangles, down); EM00212 (circles, open).

Goodman, S. L., Grote, H. J., & Wilm, C. (2012). Matched rabbit monoclonal antibodies against αv-series integrins reveal a novel αvβ3-LIBS epitope, and permit routine staining of archival paraffin samples of human tumors. Biology Open, 1(4), 329-340.

FC

Figure 2 Mouse Anti-ITGB3 Recombinant Antibody (clone EM00212) in FC

Figure 2 Mouse Anti-ITGB3 Recombinant Antibody (clone EM00212) in FC

Flow cytometry in the presence of physiological divalent cations (black open); 1 mM Mn2+ (red closed); 10 mM cilengitide in physiological cations (blue closed); 10 mM cilengitide in1 mM Mn2+ (green open). Horizontal panels show staining with EM22703 (αvβ3); EM09902 (αvβ5); EM05201 (αvβ6); and EM13309 (αvβ8). Vertical panels show staining on HUVECs, M21, A549, HT29, and M24Met. Gray shading shows binding of EM00212 and the second layer controls, which superimpose.

Goodman, S. L., Grote, H. J., & Wilm, C. (2012). Matched rabbit monoclonal antibodies against αv-series integrins reveal a novel αvβ3-LIBS epitope, and permit routine staining of archival paraffin samples of human tumors. Biology Open, 1(4), 329-340.

IHC

Figure 3 Human tumor cell lines stained on FFPE microtissue array using RabMabs.

Figure 3 Human tumor cell lines stained on FFPE microtissue array using RabMabs.

EM05201, EM13309, and EM00212, M21 and M24-met melanoma,A549 NSCLC, HT29 CRC, MDA-MB468 mammary carcinoma, and Raji Bcell lymphoma stained with EM05201 (avb6), EM13309 (avb8), and EM00212(cyto-b3). Scale bar 5 50 mm.

Goodman, S. L., Grote, H. J., & Wilm, C. (2012). Matched rabbit monoclonal antibodies against αv-series integrins reveal a novel αvβ3-LIBS epitope, and permit routine staining of archival paraffin samples of human tumors. Biology Open, 1(4), 329-340.


Specifications

  • Host Species
  • Mouse
  • Type
  • Mouse IgG1
  • Specificity
  • Cytoplasmic domain of integrin β3
  • Species Reactivity
  • Human
  • Clone
  • EM00212
  • Applications
  • IHC

Product Property

  • Purity
  • >95% as determined by SDS-PAGE
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • Integrin Subunit Beta 3

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone EM00212"

See other products for "ITGB3"

Single-domain Antibody

CAT Product Name Application Type
NAB-785-sdAb Recombinant Anti-human ITGB3 VHH Single Domain Antibody FA, FC, ICC, ELISA Llama VHH

Humanized Antibody

CAT Product Name Application Type
TAB-109-F(E) Anti-Human Integrin lpha 2b beta 3 Recombinant Antibody Fab Fragment (Tadocizumab) FC, IP, ELISA, Neut, FuncS, IF, ICC Fab - G1 - kappa

Chicken IgY Antibody

CAT Product Name Application Type
BRD-0299MZ Chicken Anti-ITGB3 Polyclonal IgY WB Chicken antibody

Blocking Antibody

Neutralizing Antibody

CAT Product Name Application Type
NEUT-1600CQ Recombinant Armenian hamster Anti-Itgb3 Antibody (2C9.G3) FC, FuncS, Neut, IF IgG
NEUT-1601CQ Recombinant Mouse Anti-ITGB3 Antibody (PM6/13) WB, Neut, ELISA, FC, ICC/IF, IHC, IHC-Fr, IHC-P, IP, ICC IgG1

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1885 Hi-Affi™ Recombinant Rabbit Anti-ITGB3 Monoclonal Antibody (DS1885AB) ICC/IF, IHC-P, WB, IHC-Fr IgG

Epitope-Specific Antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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