Mouse Anti-PTPRC Recombinant Antibody (clone 2C10C11)
CAT#: VS7-0425-WR722
This product is a mouse IgG1 antibody that binds to human PTPRC. The antibody 2C10C11 recognizes specific amino acid domain of human PTPRC (AA: 928-989). It can be used for WB and IHC assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/440/if_selected.jpg
Normal Tissue
Figure 2 Appendix
Lymphoid tissue
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/440/155132_A_3_2.jpg
Normal Tissue
Figure 3 Lymph node
Germinal center cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous
Non-germinal center cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/440/155132_A_8_8.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (Daudi, HEL, HL-60, JURKAT, MOLT-4, THP-1, U-266/70, U-266/84, U-698, U-937)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000081237-PTPRC
❮
❯
Specifications
- Immunogen
- Recombinant human PTPRC (AA: 928-989) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human PTPRC
- Species Reactivity
- Human
- Clone
- 2C10C11
- Molecule Class
- Receptor-Type Protein Tyrosine Phosphatase
- Applications
- WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Multiple sclerosis (MS); Immunodeficiency 105, severe combined (IMD105)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- PTPRC; protein tyrosine phosphatase, receptor type, C; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180; receptor-type tyrosine-protein phosphatase C; CD45 antigen; T200 glycoprotein; T200 leukocyte common antigen; protein tyrosine phosphatase, receptor typ
- Gene ID
- 5788
- UniProt ID
- M9MML4
- Long Name
- Protein tyrosine phosphatase receptor type C
- Cellular Localization
- Cell membrane; Membrane; Synapse
- Post Translation Modifications
- Glycoprotein; Phosphoprotein
- Protein Refseq
- NP_001254727.1
- Function
- Hydrolase; Protein phosphatase
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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