Human Anti-SEMA4D Recombinant Antibody (clone 21E8) (CAT#: ZG-0137U)
Human anti-SEMA4D antibody (clone 21E8) displayed high specificity against the SEMA4D protein of Human.
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Custom Production
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FC
Figure 1 Human Anti-SEMA4D Antibody (ZG-0137U) in FC
Untransfected HEK293T cells (green line) and transfected Human SEMA4D HEK293T stable cells (red line) were stained with anti-SEMA4D antibody (2µg/1*106 cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.
ELISA
Figure 2 Human Anti-SEMA4D Antibody (ZG-0137U) in ELISA
The Binding Activity of Human SEMA4D with Anti-SEMA4D recombinant antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized Human SEMA4D (ZG-0137U) at 2 μg/mL can bind Anti-SEMA4D recombinant antibody, the EC50 is 1.082-4.805 ng/mL.
Specifications
- Immunogen
- Recombinant Human SEMA4D protein
- Host Species
- Human
- Type
- Humanized IgG4
- Specificity
- Human SEMA4D
- Species Reactivity
- Human
- Clone
- 21E8
- Applications
- ELISA, FC
- Conjugate
- Unconjugated
- Related Disease
- Cholangitis, Primary Sclerosing and Autoimmune Vasculitis
Product Property
- Purification
- Affinity Purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
- Preservative
- 0.03% Proclin 300
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Flow Cytometry. Specific conditions should be optimized by the end user.
FC:1:50-1:200
Target
- Alternative Names
- A8; GR3; BB18; CD100; COLL4; SEMAJ; coll-4; C9orf164; M-sema-G; semaphorin-4D; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4D
- Gene ID
- 10507
- UniProt ID
- Q92854
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Humanized Antibodies
Rabbit Monoclonal Antibodies
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NHP Antibodies
FC Reagents & Kits
Extracellular Vesicle Antibodies
IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-559CL | Human Anti-SEMA4D Recombinant Antibody (TAB-559CL) | ELISA | Human IgG |
| TAB-429CT | Humanized Anti-SEMA4D Recombinant Antibody (TAB-429CT) | WB | Humanized IgG |
| TAB-430CT | Humanized Anti-SEMA4D Recombinant Antibody (TAB-430CT) | WB | Humanized IgG |
| TAB-429CT-S(P) | Humanized Anti-SEMA4D Recombinant Antibody; scFv Fragment (TAB-429CT-S(P)) | WB, ELISA, FC, BL | Humanized scFv |
| TAB-430CT-S(P) | Humanized Anti-SEMA4D Recombinant Antibody; scFv Fragment (TAB-430CT-S(P)) | WB, ELISA, FC, BL | Humanized scFv |
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For Research Use Only. Not For Clinical Use.
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