Human Anti-SEMA4D Recombinant Antibody (clone 21E8) (CAT#: ZG-0137U)
Human anti-SEMA4D antibody (clone 21E8) displayed high specificity against the SEMA4D protein of Human.
Specific Inquiry
FC
Figure 1 Human Anti-SEMA4D Antibody (ZG-0137U) in FC
Untransfected HEK293T cells (green line) and transfected Human SEMA4D HEK293T stable cells (red line) were stained with anti-SEMA4D antibody (2µg/1*106 cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.
ELISA
Figure 2 Human Anti-SEMA4D Antibody (ZG-0137U) in ELISA
The Binding Activity of Human SEMA4D with Anti-SEMA4D recombinant antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized Human SEMA4D (ZG-0137U) at 2 μg/mL can bind Anti-SEMA4D recombinant antibody, the EC50 is 1.082-4.805 ng/mL.
Specifications
- Immunogen
- Recombinant Human SEMA4D protein
- Host Species
- Human
- Type
- Humanized IgG4
- Specificity
- Human SEMA4D
- Species Reactivity
- Human
- Clone
- 21E8
- Applications
- ELISA, FC
- Conjugate
- Unconjugated
- Related Disease
- Cholangitis, Primary Sclerosing and Autoimmune Vasculitis
Product Property
- Purification
- Affinity Purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
- Preservative
- 0.03% Proclin 300
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Flow Cytometry. Specific conditions should be optimized by the end user.
FC:1:50-1:200
Target
- Alternative Names
- A8; GR3; BB18; CD100; COLL4; SEMAJ; coll-4; C9orf164; M-sema-G; semaphorin-4D; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4D
- Gene ID
- 10507
- UniProt ID
- Q92854
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Non-Human Primate (NHP) Antibody Products
Flow Cytometry (FC) related Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-559CL | Human Anti-SEMA4D Recombinant Antibody (TAB-559CL) | ELISA | Human IgG |
| TAB-429CT | Humanized Anti-SEMA4D Recombinant Antibody (TAB-429CT) | WB | Humanized IgG |
| TAB-430CT | Humanized Anti-SEMA4D Recombinant Antibody (TAB-430CT) | WB | Humanized IgG |
| TAB-429CT-F(E) | Humanized Anti-SEMA4D Recombinant Antibody; Fab Fragment (TAB-429CT-F(E)) | WB, ELISA, FC, BL | Humanized Fab |
| TAB-430CT-F(E) | Humanized Anti-SEMA4D Recombinant Antibody; Fab Fragment (TAB-430CT-F(E)) | WB, ELISA, FC, BL | Humanized Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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