Mouse Anti-VIM Recombinant Antibody (clone RV204) (CAT#: ZG-1102F)

This product is a mouse antibody that recognizes VIM.

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IF

Figure 1 Mouse Anti-VIM Antibody (ZG-1102F) in IF

Figure 1 Mouse Anti-VIM Antibody (ZG-1102F) in IF

Indirect immunofluorescence staining of normal human dermal fibroblasts in tissue culture with the antibody (diluted 1:250), showing the specific cytoskeletal pattern of vimentin intermediate filaments.

WB

Figure 10 Mouse Anti-VIM Antibody (ZG-1102F) in WB

Figure 10 Mouse Anti-VIM Antibody (ZG-1102F) in WB

Western blotting result showing the specific reactivity of the antibody with the 57kDa protein band of vimentin in both the mouse (3T3 mouse fibroblasts; left lane) and human (normal human dermal fibroblasts; right lane) cell extracts.

SDS-PAGE

Figure 11 Mouse Anti-VIM Antibody (ZG-1102F) in SDS-PAGE

Figure 11 Mouse Anti-VIM Antibody (ZG-1102F) in SDS-PAGE

Non-reduced and reduced SDS-PAGE of the antibody showing its purity.

IF

Figure 2 Mouse Anti-VIM Antibody (ZG-1102F) in IF

Figure 2 Mouse Anti-VIM Antibody (ZG-1102F) in IF

Indirect immunofluorescence staining of human kidney tissue section with the antibody (diluted 1:1000), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue, podocytes, and endothelial cells in blood vessels. As expected, no reactivity is seen in the epithelial cell compartment

IF

Figure 3 Mouse Anti-VIM Antibody (ZG-1102F) in IF

Figure 3 Mouse Anti-VIM Antibody (ZG-1102F) in IF

Indirect immunofluorescence staining of human kidney tissue section with the antibody (diluted 1:1000), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue, podocytes, and endothelial cells in blood vessels. As expected, no reactivity is seen in the epithelial cell compartment

IHC

Figure 4 Mouse Anti-VIM Antibody (ZG-1102F) in IHC

Figure 4 Mouse Anti-VIM Antibody (ZG-1102F) in IHC

Immunostaining of human paraffin embedded tissue section of placenta with the antibody (diluted 1:100), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue, and endothelial cells in blood vessels. As expected, no reactivity is seen in the epithelial cell compartment

IHC

Figure 5 Mouse Anti-VIM Antibody (ZG-1102F) in IHC

Figure 5 Mouse Anti-VIM Antibody (ZG-1102F) in IHC

Immunostaining of human paraffin embedded tissue section of testis with the antibody (diluted 1:100), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue, endothelial cells in blood vessels and Sertoli cells.

IHC

Figure 6 Mouse Anti-VIM Antibody (ZG-1102F) in IHC

Figure 6 Mouse Anti-VIM Antibody (ZG-1102F) in IHC

Immunostaining of human paraffin embedded tissue sections of human colon with the antibody (diluted 1:100), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue, and endothelial cells in blood vessels. As expected, no reactivity is seen in the epithelial cell compartment.

IHC

Figure 7 Mouse Anti-VIM Antibody (ZG-1102F) in IHC

Figure 7 Mouse Anti-VIM Antibody (ZG-1102F) in IHC

Immunostaining of human paraffin embedded tissue sections of human kidney with the antibody (diluted 1:100), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue, and podocytes. As expected, no reactivity is seen in the epithelial cell compartment.

IHC

Figure 8 Mouse Anti-VIM Antibody (ZG-1102F) in IHC

Figure 8 Mouse Anti-VIM Antibody (ZG-1102F) in IHC

Immunostaining of human paraffin embedded tissue sections of human small intestine with the antibody (diluted 1:100), showing the specific pattern of vimentin in the mesenchymal cell types, such as fibroblasts in the connective tissue. As expected, no reactivity is seen in the epithelial cell compartment.

IHC

Figure 9 Mouse Anti-VIM Antibody (ZG-1102F) in IHC

Figure 9 Mouse Anti-VIM Antibody (ZG-1102F) in IHC

Immunostaining of human paraffin embedded tissue sections of human spleen with the antibody (diluted 1:100), showing the specific pattern of vimentin in the mesenchymal cell types.


Specifications

  • Host Species
  • Mouse
  • Type
  • Mouse IgG1
  • Specificity
  • Human and mouse vimentin
  • Species Reactivity
  • Human, Mouse
  • Clone
  • RV204
  • Applications
  • Immunohistochemistry, Immunocytochemistry, Immunoblotting

Product Property

  • Format
  • Liquid
  • Buffer
  • PBS
  • Preservative
  • 0.09% Sodium azide
  • Storage
  • Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.

Target

  • Alternative Names
  • Vimentin

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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