CytoStream™ Mouse Anti-CD20 Recombinant Antibody (MMC-M1133)
CAT#: VS-0126-XU187
CytoStream™ Mouse Anti-CD20 Recombinant Antibody (MMC-M1133) is an optimized reagent for the robust identification of human B-lymphocyte lineages. This recombinant antibody recognizes a specific extracellular epitope with high precision, providing sharp and bright identification of B cells in various biological samples. Its recombinant manufacture ensures unmatched lot-to-lot reproducibility, eliminating the signal drift often seen with traditional hybridoma lines. It is an essential component for standardized immunophenotyping, allowing for the stable and accurate quantification of B-cell compartments in both basic and clinical research settings.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line REH
Immunofluorescent staining of human cell line REH shows localization to nucleoplasm & plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/1778_G3_5_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/201_H5_1_red_green.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/202_H5_1_red_green.jpg
Normal Tissue
Figure 4 Colon
Mucosal lymphoid cells
Staining:Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/39081_A_7_3.jpg
Normal Tissue
Figure 5 Bone marrow
Hematopoietic cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/39081_B_4_4.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14341/39081_A_7_8.jpg
RNA Expression
Figure 7 RNA cell line category: Group enriched (Daudi, U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000156738-MS4A1
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| Sub Cat | Product Name | Size | Formulation | Conjugate Group | Characteristics | Stability | Size | Conjugation | Endotoxin | Purity | Fc Engineering | |
| VS-0126-XU187-1 | CytoStream™ Mouse Anti-CD20 Recombinant Antibody (MMC-M1133), APC-conjugated | 25 Tests | Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4. | APC | Protein-based fluorochrome with excitation/emission at ~650/660 nm. Exhibits minimal spectral overlap with FITC and PE, ideal for multicolor panels. High brightness. | Sensitive to light and heat. Store at 4°C in the dark. Avoid repeated freeze-thaw cycles. Stable for ≥1 year under proper storage. | ||||||
| VS-0126-XU187-2 | CytoStream™ Mouse Anti-CD20 Recombinant Antibody (MMC-M1133), PE-Cy5-conjugated | 25 Tests | Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4. | PE-Cy5 | Tandem dye where PE transfers energy to Cy5 (Em ~667 nm). Critical: Use a Cy5 detector, not a PE filter. Prone to degradation of the tandem bond, causing uncoupled PE signal. | Low stability. Highly sensitive to fixation, light, and prolonged storage. Protect from light and analyze fixed samples promptly. | ||||||
| VS-0126-XU187-3 | CytoStream™ Mouse Anti-CD20 Recombinant Antibody (MMC-M1133), FITC-conjugated | 25 Tests | Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4. | FITC | Small organic fluorophore (Ex/Em: 488/519 nm). High initial brightness but susceptible to photobleaching. Requires careful compensation with PE channel. | Sensitive to light and pH. Prone to rapid photobleaching during acquisition. Store at 4°C in the dark. Prepare working solutions fresh. | ||||||
| VS-0126-XU187-4 | CytoStream™ Mouse Anti-CD20 Recombinant Antibody (MMC-M1133), PE-conjugated | 25 Tests | Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4. | PE | Phycobiliprotein fluorochrome (Ex/Em peak: 565/575 nm). One of the brightest fluorophores available, ideal for low-abundance antigen detection. Broad emission requires careful compensation. | Moderate stability. Sensitive to light, heat, and harsh fixatives. Store at 4°C in the dark. Avoid freeze-thaw cycles to maintain performance. | ||||||
| VS-0126-XU187-5 | CytoStream™ Mouse Anti-CD20 Recombinant Antibody (MMC-M1133), Pacific Blue-conjugated | 25 Tests | Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4. | Pacific Blue | Synthetic blue fluorescent dye (Ex/Em: ~410/455 nm). Excellent for violet laser (405 nm) excitation. Low spectral overlap with green/red channel fluorophores, simplifying panel design. | High photostability. Tolerates common fixation protocols well. Store at 4°C in the dark for long-term stability. | ||||||
| VS-0126-XU187-6 | CytoStream™ Mouse Anti-CD20 Recombinant Antibody (MMC-M1133), PE-Cy7-conjugated | 25 Tests | Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4. | PE-Cy7 | Tandem dye (PE excited, Cy7 emits at ~785 nm). Critical for deep-red infrared detection. Essential: Use a Cy7 (780/60 nm) detector, not a PE filter. Susceptible to tandem bond degradation. | Low stability. Highly sensitive to fixation, light exposure, and storage time. Protect from light and analyze immediately post-fixation. | ||||||
| VS-0126-XU187-7 | CytoStream™ Mouse Anti-CD20 Recombinant Antibody (MMC-M1133), APC-Cy7-conjugated | 25 Tests | Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4. | APC-Cy7 | Tandem dye (APC excited, Cy7 emits at ~785 nm). Provides a bright, far-red to infrared signal. Shares the APC-Cy7 detection channel with other Cy7 tandems. Also prone to bond degradation under stress. | Low stability. Similar sensitivity as PE-Cy7 to fixation and light. Requires strict adherence to dark, cold storage and prompt analysis. | ||||||
| VS-0126-XU187-8 | CytoStream™ Purified Mouse Anti-CD20 Recombinant Antibody (MMC-M1133) | 100 ug | None | The native antibody without any fluorophore. Used as a primary antibody in indirect staining or for functional assays (blocking, neutralization). Requires a compatible fluorescent secondary antibody for detection. | Typically exhibits the highest intrinsic stability among all formats. Storage conditions are defined by the antibody's own formulation buffer. |
More Infomation
Specifications
- Host Species
- Mouse
- Type
- Monoclonal Antibody
- Specificity
- CD20
- Species Reactivity
- Human
- Applications
- FC
Product Property
- Clonality
- Monoclonal
- Storage
- Store at 4˚C.
- Shipping
- Ice Packs
Applications
- Application Notes
- Test: 5 μl/Test
Notice: This reagent is supplied in a pre-optimized, ready-to-use formulation for direct application at the specified volume per test in flow cytometry. The standard protocol is suitable for staining up to 1 million cells per assay. Optimal performance may require protocol adjustments based on specific experimental conditions.
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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| Gly-134LC-1 | Recombinant Anti-Human MS4A1 Antibody (Fc glycosylation/Non fucosylated) | ELISA | Humanized antibody |
| Gly-135LC-1 | Recombinant Anti-Human MS4A1 Antibody (Fc glycosylation/Non fucosylated) | ELISA | Humanized antibody |
| Gly-136LC-1 | Recombinant Anti-Human MS4A1 Antibody (Fc glycosylation/Non fucosylated) | ELISA | Humanized antibody |
| Gly-137LC-1 | Recombinant Anti-Human MS4A1 Antibody (Fc glycosylation/Non fucosylated) | ELISA | Humanized antibody |
| Gly-138LC | Recombinant Anti-Human MS4A1 Antibody (Fc glycosylation/Non fucosylated) | ELISA | Humanized antibody |
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| Gly-142LC-1 | Recombinant Anti-Human MS4A1 Antibody (Fc glycosylation/Hyper-galactosylated) | ELISA | Human antibody |
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| Gly-155LC-1 | Recombinant Anti-Human MS4A1 Antibody (Fc glycosylation/High-mannose glycosylated) | ELISA | Humanized antibody |
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| Gly-171LC | Recombinant Anti-Human MS4A1 Antibody (Non-glycosylated) | ELISA | Chimeric antibody (mouse/human) |
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| MOB-0279MZ | Mouse Anti-MS4A1 Recombinant Antibody (clone M27 (1, 4).) | IHC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| MHC-LC067 | APC-A*02:01/Human CD20 (SLFLGILSV) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
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| MHC-LC068 | BV421-A*02:01/Human CD20 (SLFLGILSV) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
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| NEUT-1751CQ | Mouse Anti-MS4A1 Recombinant Antibody (clone CBLS-211) | Neut, FuncS | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
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| MOR-2295 | Hi-Affi™ Rabbit Anti-MS4A1 Recombinant Antibody (clone DS2295AB) | IHC-Fr, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| MOR-4024 | Hi-Affi™ Rabbit Anti-MS4A1 Recombinant Antibody (clone SI88DS) | FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| MHC-LC4347 | PE-B*07:02/Human MS4A1 (RPKSNIVLL) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0241CQ-F(E) | Mouse Anti-MS4A1 Recombinant Antibody (clone 2H7); Fab Fragment | ELISA, FC, IHC, IP | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0241CQ-S(P) | Mouse Anti-MS4A1 Recombinant Antibody (clone 2H7); scFv Fragment | ELISA, FC, IHC, IP | Mouse scFv |
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|---|---|---|---|
| HPAB-M0704-YC | Camelid Anti-MS4A1 Recombinant Antibody (HPAB-M0704-YC) | ELISA, FuncS | Camelid VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0705-YC | Camelid Anti-MS4A1 Recombinant Antibody (HPAB-M0705-YC) | ELISA | Camelid VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0706-YC | Camelid Anti-MS4A1 Recombinant Antibody (HPAB-M0706-YC) | ELISA | Camelid VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-M0707-YC | Camelid Anti-MS4A1 Recombinant Antibody (HPAB-M0707-YC) | ELISA | Camelid VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-772 | Afuco™ Anti-MS4A1 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-772) | ELISA, IP, FC, FuncS, Neut, IF | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-016 | Afuco™ Anti-MS4A1 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-016) | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-028 | Afuco™ Anti-MS4A1 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-028) | FC, IP, ELISA, Neut, FuncS, IF | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-207 | Afuco™ Anti-MS4A1 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-207) | ELISA, IP, FC, FuncS, Neut, IF | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-771 | Afuco™ Anti-MS4A1 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-771) | IF, IP, Neut, FuncS, ELISA, FC | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY188 | AbPlus™ Anti-MS4A1 Magnetic Beads (VS-0424-XY188) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC138 | Human Anti-MS4A1 Recombinant Antibody Hexamer (VS-0924-YC138), CDC Enhanced | FC, CDC, IP | Antibody hexamer |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC139 | Mouse Anti-MS4A1 Recombinant Antibody Hexamer (VS-0924-YC139), CDC Enhanced | FC, Activ, Agonist, IF, WB, Cyt, CDC | Antibody hexamer |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY85 | Mouse Anti-NHP MS4A1 Recombinant Antibody (VS-1024-XY85) | FC | Mouse IgG2b, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0125-FY30 | Human Anti-MS4A1 (clone RW005) scFv-Fc Chimera | FuncS | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY168 | CytoStream™ Rat Anti-MS4A1 Recombinant Antibody (clone GOT214A) | FC | Rat IgG2a, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC513 | Recombinant Anti-MS4A1 Vesicular Antibody, EV Displayed (VS-0425-YC513) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4510 | Anti-MS4A1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4511 | Anti-Mouse MS4A1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC129 | Recombinant Anti-MS4A1 (Bin I x Bin II) Biparatopic Antibody, Tandem scFv | ELISA, FC | Tandem scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC222 | Recombinant Anti-MS4A1 Biparatopic Antibody, Tandem scFv | ELISA, IF, WB | Tandem scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0825-YC17 | SmartAb™ Recombinant Anti-MS4A1 pH-dependent Antibody (VS-0825-YC17) | FC, Inhib | Human IgG1 kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0825-YC257 | SmartAb™ Recombinant Anti-MS4A1 pH-dependent Antibody (Clone Rituximab) | IF, IP, Neut, ELISA, FC, WB | Human IgG1 kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1025-YC1 | Anti-MS4A1 Antibody Prodrug, Protease Activated (Rituximab) | ISZ, Cyt, FuncS |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1025-YC158 | Anti-MS4A1 Antibody Prodrug, Protease Activated (2H7) | ISZ, Cyt, FuncS |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1125-XY645 | Rabbit Anti-MS4A1 Recombinant Antibody (VS-1125-XY645) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1125-XY646 | Mouse Anti-MS4A1 Recombinant Antibody (VS-1125-XY646) | WB, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1125-XY647 | Mouse Anti-MS4A1 Recombinant Antibody (VS-1125-XY647) | IHC, ICC, IF, FC, ELISA, IP | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1125-XY648 | Rabbit Anti-MS4A1 Recombinant Antibody (VS-1125-XY648) | WB, IHC, ICC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1125-XY649 | Rabbit Anti-MS4A1 Recombinant Antibody (VS-1125-XY649) | IHC | Rabbit IgG |
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