Recombinant Rabbit Anti-ATF2 Antibody (clone R09-8F4)
CAT#: VS3-FY102
This product is a recombinant rabbit antibody that recognizes human ATF2 protein. It can detect a band of approximately 55 kDa (observedd molecular weight: 70 kDa).
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22134/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human prostate
Immunohistochemical staining of human prostate shows moderate nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22134/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows strong nuclear positivity in Purkinje cells and cells in molecular and granular layer.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_B_7_8_selected.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_A_8_8.jpg
Normal Tissue
Figure 6 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_B_9_5.jpg
Normal Tissue
Figure 7 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_A_9_3.jpg
Normal Tissue
Figure 8 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_A_9_4.jpg
Normal Tissue
Figure 9 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_A_7_5.jpg
Normal Tissue
Figure 10 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3769/11607_A_4_6.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000115966-ATF2
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WB
Figure 1. Recombinant Rabbit Anti-ATF2 Antibody (clone R09-8F4) in WB.
Western blot analysis of ATF2 in Jurkat lysates using ATF2 antibody.
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Specifications
- Immunogen
- A synthetic peptide of human ATF2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human ATF2
- Species Reactivity
- Human
- Clone
- R09-8F4
- Applications
- Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
- Conjugate
- Unconjugated
- MW
- Calculated MW: 55 kDa; Observed MW: 70 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95%
- Format
- Liquid
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% Glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Activating Transcription Factor 2; Cyclic AMP-Responsive Element-Binding Protein 2; CAMP Response Element-Binding Protein CRE-BP1; CAMP-Dependent Transcription Factor ATF-2; Histone Acetyltransferase ATF2; CREB-2; CREB2; HB16; Activating Transcription Factor 2 Splice Variant ATF2-Var2
- Gene ID
- 1386
- UniProt ID
- P15336
- Sequence Similarities
- Belongs to the bZIP family. ATF subfamily.
- Cellular Localization
- Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus
- Post Translation Modifications
- Phosphorylation of Thr-69 by MAPK14 and MAPK11, and at Thr-71 by MAPK1/ERK2, MAPK3/ERK1, MAPK11, MAPK12 and MAPK14 in response to external stimulus like insulin causes increased transcriptional activity (PubMed:9430721, PubMed:12110590).
Phosphorylated by PLK3 following hyperosmotic stress (PubMed:21098032).
Also phosphorylated and activated by JNK and CaMK4 (PubMed:8855261).
ATM-mediated phosphorylation at Ser-490 and Ser-498 stimulates its function in DNA damage response (PubMed:15916964).
Phosphorylation at Ser-62, Thr-73 and Ser-121 activates its transcriptional activity (PubMed:15105425).
Phosphorylation at Thr-69 or Thr-71 enhances acetylation of histones H2B and H4 (PubMed:10821277).
- Protein Refseq
- NP_001243019.1; NP_001243020.1; NP_001243021.1
- Function
- Transcriptional activator which regulates the transcription of various genes, including those involved in anti-apoptosis, cell growth, and DNA damage response. Dependent on its binding partner, binds to CRE (cAMP response element) consensus sequences (5'-TGACGTCA-3') or to AP-1 (activator protein 1) consensus sequences (5'-TGACTCA-3'). In the nucleus, contributes to global transcription and the DNA damage response, in addition to specific transcriptional activities that are related to cell development, proliferation and death. In the cytoplasm, interacts with and perturbs HK1- and VDAC1-containing complexes at the mitochondrial outer membrane, thereby impairing mitochondrial membrane potential, inducing mitochondrial leakage and promoting cell death. The phosphorylated form (mediated by ATM) plays a role in the DNA damage response and is involved in the ionizing radiation (IR)-induced S phase checkpoint control and in the recruitment of the MRN complex into the IR-induced foci (IRIF). Exhibits histone acetyltransferase (HAT) activity which specifically acetylates histones H2B and H4 in vitro (PubMed:10821277).
In concert with CUL3 and RBX1, promotes the degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. Can elicit oncogenic or tumor suppressor activities depending on the tissue or cell type.
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Related Signaling Pathways
BCR Signaling Pathway
Canonical Wnt Signaling Pathway
MAPK Signaling Pathway
Non-Canonical Wnt Signaling Pathway
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "ATF2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0274 | Hi-Affi™ Rabbit Anti-ATF2 Recombinant Antibody (clone DS274AB) | IF, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0130-CN | Rabbit Anti-ATF2 Recombinant Antibody (clone CBACN-047) | WB, IF, IHC, IP | Rabbit IgG |
| ZG-0315F | Mouse Anti-ATF2 Recombinant Antibody (ZG-0315F) | WB, IHC, ELISA | Mouse IgG |
| VS3-CJ672 | Rabbit Anti-ATF2 Recombinant Antibody (VS3-CJ672) | WB, ICC, IF, IHC, IP | Rabbit IgG |
| VS3-FY1638 | Rabbit Anti-Atf2 Recombinant Antibody (clone R01-9F9) | WB | Rabbit IgG |
| VS7-HM145 | Mouse Anti-ATF2 Recombinant Antibody (clone CBL055HM) | WB, IHC-p, IF, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY22 | AbPlus™ Anti-ATF2 Magnetic Beads (CBACN-047) | IP, Protein Purification |
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