Recombinant Rabbit Anti-NTRK2 (phospho Tyr516) Antibody (clone R04-3D9)
CAT#: VS3-FY1073
This product is a recombinant rabbit antibody that recognizes NTRK2. This antibody has been reported for use in Western Blot. The clone R04-3D9 is specific for human, mouse, rat NTRK2. The antigen is a synthetic phosphopeptide corresponding to residues surrounding tyr516 of human trkb.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7637/202_C1_1_selected.jpg
Normal Tissue
Figure 2 High expression in cerebral cortex
Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10346/162989_B_7_5.jpg
Normal Tissue
Figure 3 Kidney
Cells in tubules Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10346/162989_A_8_5.jpg
Normal Tissue
Figure 4 Testis
Leydig cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10346/162989_A_6_6.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (AF22, HaCaT, RPTEC TERT1, SH-SY5Y, SuSa)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000148053-NTRK2
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WB
Figure 1. Recombinant Rabbit Anti-NTRK2 (phospho Tyr516) Antibody (clone R04-3D9) in WB.
Western Blot analysis of Phospho-TrkA (tyr490)/TrkB (Tyr516) in K562, C6, 3T3 lysates using Phospho-TrkA/B (Tyr490/Tyr516) Antibody.
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Specifications
- Immunogen
- A synthetic phosphopeptide corresponding to residues surrounding Tyr516 of human TrkB.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat NTRK2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R04-3D9
- Applications
- Western Blot
- Conjugate
- Unconjugated
- MW
- Calculated MW: 92 kDa; Observed MW: 140 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Neurotrophic Receptor Tyrosine Kinase 2; Neurotrophic Tyrosine Kinase Receptor Type 2; Tropomyosin-Related Kinase B; EC 2.7.10.1; GP145-TrkB; Trk-B; TRKB; Neurotrophic Tyrosine Kinase, Receptor, Type 2
- Gene ID
- 4915
- UniProt ID
- Q16620
- Cellular Localization
- Cell membrane, Cell projection, Cytoplasm, Endosome, Membrane, Synapse
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Related Diseases
EGFR Tyrosine Kinase Inhibitor Resistance
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| NS-095CN-F(E) | Human Anti-NTRK2 Recombinant Antibody; Fab Fragment (NS-095CN-F(E)) | ELISA | Human Fab |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0662-CN | Human Anti-NTRK2 Recombinant Antibody (HPAB-0662-CN) | ELISA, Neut | Humanized IgG |
| HPAB-0663-CN | Human Anti-NTRK2 Recombinant Antibody (HPAB-0663-CN) | ELISA, Neut | Humanized IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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