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    Mouse Anti-EGFR Recombinant Antibody (clone 7A7); Fab Fragment (CAT#: PFBW-088)

    Recombinant Mouse Antibody (7A7) is capable of binding to Human EGFR, expressed in Chinese Hamster Ovary cells (CHO). 7A7 recognizes a site on the EGFR extracellular domain that is not accessible in its most stable conformations, but that becomes exposed upon treatment with a tyrosine kinase inhibitor.

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
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    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line A-431
    Figure 1 IF staining of human cell line A-431
    (Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cell junctions.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/144_E9_2_selected.jpg
    Figure 2 IF staining of human cell line U-251 MG
    Figure 2 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/143_E9_1_blue_red_green.jpg
    Normal Tissue
    Figure 3 Colon
    Figure 3 Colon
    (Glandular cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_3.jpg
    Figure 4 Liver
    Figure 4 Liver
    (Cholangiocytes
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_4.jpg
    Figure 5 Kidney
    Figure 5 Kidney
    (Bowman's capsule
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Collecting ducts
    Staining:Medium
    Intensity: Strong
    Quantity: <25%
    Distal tubules
    Staining:Medium
    Intensity: Strong
    Quantity: <25%
    Proximal tubules (cell body)
    Staining:Medium
    Intensity: Strong
    Quantity: <25%)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_5.jpg
    Figure 6 Testis
    Figure 6 Testis
    (Leydig cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Pachytene spermatocytes
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Round or early spermatids
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_6_6.jpg
    Figure 7 Placenta
    Figure 7 Placenta
    (Cytotrophoblasts
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Decidual cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Hofbauer cells
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Syncytiotrophoblasts - cell body
    Staining:High
    Intensity: Strong
    Quantity:>75%
    Syncytiotrophoblasts - microvilli
    Staining:High
    Intensity: Strong)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_1_7.jpg
    Figure 8 Lymph node
    Figure 8 Lymph node
    (Non-germinal center cells
    Staining:Medium
    Intensity: Strong
    Quantity: <25%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_8.jpg
    RNA Expression
    Figure 9 RNA cell line category: Cell line enriched (A-431)
    Figure 9 RNA cell line category: Cell line enriched (A-431)
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000146648-EGFR

    Specifications

    • Immunogen
    • Human epidermal growth factor receptor
    • Host Species
    • Mouse
    • Type
    • Mouse Fab
    • Specificity
    • Human EGFR
    • Species Reactivity
    • Human
    • Clone
    • 7A7
    • Applications
    • ELISA, WB

    Product Property

    • Purity
    • >95% as determined by analysis by SDS-PAGE
    • Concentration
    • Please refer to the vial label for the specific concentration.
    • Storage
    • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

    Target

    • Alternative Names
    • ERBB; ERRP; HER1; mENA; ERBB1; PIG61; NISBD2; epidermal growth factor receptor; EGFR vIII; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; epidermal growth factor receptor tyrosine kinase domain; erb-b2 receptor tyrosine kinase 1; proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1

    Related Resources

    • Related Diseases
    • Related Signaling Pathways

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

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