Mouse Anti-EGFR Recombinant Antibody (clone 7A7); scFv Fragment (CAT#: PSBW-088)

Recombinant Mouse Antibody (7A7) is capable of binding to Human EGFR, expressed in Chinese Hamster Ovary cells (CHO). 7A7 recognizes a site on the EGFR extracellular domain that is not accessible in its most stable conformations, but that becomes exposed upon treatment with a tyrosine kinase inhibitor.

Host: Mouse

Isotype: Mouse scFv

Applications: ELISA, WB


Specific Inquiry
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  • Conjugation:
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  • Purity:
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  • Gene Expression
  • Datasheet
  • MSDS
  • COA

Subcellular Location and Protein Expression

Normal Tissue

RNA Expression


Specifications

  • Immunogen
  • Human epidermal growth factor receptor
  • Host Species
  • Mouse
  • Type
  • Mouse scFv
  • Specificity
  • Human EGFR
  • Species Reactivity
  • Human
  • Clone
  • 7A7
  • Applications
  • ELISA, WB

Product Property

  • Purity
  • >95% as determined by analysis by SDS-PAGE
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The EGFR antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Flow Cytometry, Western Blot, Immunofluorescence. Flow Cytometry: Cells were prepared for flow cytometry by washing with PBS and then FACs buffer (2% FBS in PBS). Cell suspensions (1x10^6cells) were stained with 7A7, 7A7 Fc Silent, cetuximab or AF1280 antibodies, and incubated with Alexa 488-conjugated-anti-mouse, anti-human or anti-goat secondary antibodies respectively. Immunofluorescence: Sections were then incubated with either 7A7, 7A7 Fc Silent, cetuximab or AF1280 antibody overnight at 4°C. After washing with PBS, sections were incubated with Alexa-Fluor-594 conjugated anti-mouse, anti-human or anti-goat secondary antibodies respectively. Western Blot: PVDF membranes were blocked with 2% BSA in PBS and incubated with 7A7, 7A7 Fc Silent, anti-mouse EGFR "AF1280" or β-tubulin primary antibodies and anti-mouse or anti-goat HRP secondary antibodies.

Target

  • Alternative Names
  • ERBB; ERRP; HER1; mENA; ERBB1; PIG61; NISBD2; epidermal growth factor receptor; EGFR vIII; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; epidermal growth factor receptor tyrosine kinase domain; erb-b2 receptor tyrosine kinase 1; proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1

Related Resources

  • Related Diseases
  • Related Signaling Pathways

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "Clone 7A7"

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Single-domain Antibody

CAT Product Name Application Type
NABG-056 Recombinant Anti-Mouse Egfr VHH Single Domain Antibody ELISA, IHC, FC, FuncS Llama VHH
PNBL-016 Recombinant Anti-Human EGFR VHH Single Domain Antibody (7D12) WB, ELISA Llama VHH
PNBL-017 Recombinant Anti-Human EGFR VHH Single Domain Antibody (9G8) FuncS, ELISA, IF Llama VHH
PNBL-018 Recombinant Anti-Human EGFR VHH Single Domain Antibody (EgA1) FuncS, SPR Llama VHH
TAB-015MZ-VHH Anti-Human EGFR Recombinant Antibody (PMP7A5) sELISA Single domain antibody

Chimeric Antibody

CAT Product Name Application Type
TAB-H35 Anti-Human EGFR Recombinant Antibody (Futuximab) IF, WB, Inhib IgG1 - kappa
TAB-003 Anti-Human EGFR Recombinant Antibody (Cetuximab) IF, IP, Neut, FuncS, ELISA, FC, ICC IgG1 - kappa
TAB-H49 Anti-Human EGFR Recombinant Antibody (Modotuximab) FuncS, IF, Neut, ELISA, FC, IP, IHC IgG1 - kappa
TAB-272MZ-F(E) Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-272MZ-F(E)) FuncS Chimeric (mouse/human) Fab
TAB-302MZ-F(E) Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-302MZ-F(E)) ELISA Chimeric (mouse/human) Fab

Humanized Antibody

CAT Product Name Application Type
TAB-165 Anti-Human EGFR Recombinant Antibody (Matuzumab) Neut, ELISA, IF, IP, FuncS, FC, ICC IgG1
TAB-710 Anti-EGFR Recombinant Antibody (Nimotuzumab) ELISA, IP, FC, FuncS, Neut, IF, IHC IgG1 - kappa
TAB-274MZ-S(P) Human Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-274MZ-S(P)) FC Humanized scFv
TAB-297MZ-S(P) Anti-Human EGFR Recombinant Antibody scFv Fragment (H225) ELISA, WB Humanized antibody
TAB-298MZ-S(P) Anti-Human EGFR Recombinant Antibody scFv Fragment (Hu225) ELISA, WB Humanized antibody

Recombinant Antibody

CAT Product Name Application Type
PABW-171 Mouse Anti-EGFR Recombinant Antibody (PABW-171) WB Mouse IgG
PFBW-088 Mouse Anti-EGFR Recombinant Antibody (clone 7A7); Fab Fragment ELISA, WB Mouse Fab
MOB-0094MZ Recombinant Mouse Anti-EGFR Antibody (clone FGF-R2) ELISA, IHC-P, FC Mouse antibody
FAMAB-0094YC Human Anti-EGFR Recombinant Antibody (clone F4) ELISA, WB, FC Human IgG
HPAB-0044-FY Human Anti-EGFR Recombinant Antibody (clone TGM10-1) FC Humanized IgG

Mouse Antibody

CAT Product Name Application Type
TAB-271MZ-S(P) Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-271MZ-S(P)) FuncS Mouse scFv
TAB-303MZ-S(P) Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-303MZ-S(P)) ELISA Mouse scFv
TAB-304MZ-S(P) Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-304MZ-S(P)) ELISA Mouse scFv
TAB-305MZ-S(P) Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-305MZ-S(P)) ELISA Mouse scFv
TAB-306MZ-S(P) Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-306MZ-S(P)) ELISA Mouse scFv

Human Antibody

CAT Product Name Application Type
TAB-299MZ-S(P) Human Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-299MZ-S(P)) Internalization Assay Human scFv
TAB-299MZ-F(E) Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-299MZ-F(E)) Internalization Assay Human Fab
TAB-313MZ-F(E) Anti-Human EGFR Recombinant Antibody Fab Fragment (EG-19-11) ELISA, FC Human antibody
TAB-314MZ-F(E) Anti-Human EGFR Recombinant Antibody Fab Fragment (EG-26-11) ELISA, FC Human antibody
PABX-053 Recombinant Human Anti-EGFR Antibody (DL11) WB, ELISA, FuncS IgG

Fc Glycosylation

CAT Product Name Application Type
Gly-055LC Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated) ELISA Chimeric antibody (mouse/human)
Gly-144LC Recombinant Anti-Human EGFR Antibody (Fc glycosylation) ELISA Humanized antibody

High-mannose Glycoform

CAT Product Name Application Type
Gly-055LC-1 Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated) ELISA Chimeric antibody (mouse/human)

Deglycosylated Antibody (Non-glycosylated IgGs)

CAT Product Name Application Type
Gly-167LC Recombinant Anti-Human EGFR Antibody (Non-glycosylated) ELISA Human antibody

Chicken IgY Antibody

CAT Product Name Application Type
BRD-0183MZ Chicken Anti-EGFR Polyclonal IgY WB Chicken antibody
BRD-0668MZ Chicken Anti-EGFR Polyclonal IgY WB Chicken antibody

MHC Tetramer for Cancer

CAT Product Name Application Type
MHC-LC773 A*0201/Human EGFR (YLNTVQPTCV) MHC Tetramer FCM
MHC-LC4545 PE-DQB1*03:02/Human EGFR (SRALEEKKGNYVVTHG) MHC Tetramer FCM
MHC-YF409 A*02:01/Human EGF-R-479 (KLFGTSGQKT) MHC Pentamer FCM

Neutralizing Antibody

CAT Product Name Application Type
NEUT-722CQ Rabbit Anti-EGFR Recombinant Antibody (clone CBL1011) Neut Rabbit IgG
NEUT-723CQ Mouse Anti-EGFR Recombinant Antibody (clone CBL931) WB, IP, IHC, ICC, Neut Mouse IgG1
NEUT-724CQ Rabbit Anti-EGFR Recombinant Antibody (clone D1D4J) IF, FC, WB, IP, Neut Rabbit IgG

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1101 Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone DS1101AB) IHC-P Rabbit IgG
MOR-4520 Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH28DS) IF, ICC, FC Rabbit IgG
MOR-4570 Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH82DS) ELISA Rabbit IgG
MOR-4571 Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH83DS) WB, IF, ICC, FC Rabbit IgG
MOR-0033-FY Rabbit Anti-EGFR Recombinant Antibody (clone AFY0004) ICC, IHC, WB Rabbit IgG

Fab Fragment Antibody

CAT Product Name Application Type
HPAB-1443LY-F(E) Human Anti-EGFR Recombinant Antibody (clone P1X); Fab Fragment ELISA, WB Human Fab
HPAB-1444LY-F(E) Human Anti-EGFR Recombinant Antibody (clone P2X); Fab Fragment ELISA, WB Human Fab
HPAB-1445LY-F(E) Human Anti-EGFR Recombinant Antibody (clone P3X); Fab Fragment ELISA, WB Human Fab
HPAB-1446LY-F(E) Human Anti-EGFR Recombinant Antibody (clone 25E); Fab Fragment ELISA, WB Human Fab
HPAB-2194LY-F(E) Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (HPAB-2194LY-F(E)) ELISA, WB Mouse Fab

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-165 Afuco™ Anti-EGFR ADCC Recombinant Antibody (Matuzumab), ADCC Enhanced Neut, ELISA, IF, IP, FuncS, FC ADCC enhanced antibody
AFC-TAB-464CQ Afuco™ Anti-EGFR ADCC Recombinant Antibody (Tomuzotuximab), ADCC Enhanced ELISA, IHC, FC, IP, IF, FuncS ADCC enhanced antibody
AFC-TAB-003 Afuco™ Anti-EGFR ADCC Recombinant Antibody (Cetuximab), ADCC Enhanced IF, IP, Neut, FuncS, ELISA, FC ADCC enhanced antibody
AFC-TAB-040 Afuco™ Anti-EGFR ADCC Recombinant Antibody (Zalutumumab), ADCC Enhanced ELISA, FC, IP, FuncS, IF, Neut ADCC enhanced antibody
AFC-TAB-119 Afuco™ Anti-EGFR ADCC Recombinant Antibody (Necitumumab), ADCC Enhanced FC, IP, ELISA, Neut, FuncS, IF ADCC enhanced antibody

scFv Fragment Antibody

CAT Product Name Application Type
HPAB-0894-WJ-S(P) Human Anti-EGFR Recombinant Antibody (clone YZ-EGFR V1); scFv Fragment ELISA, WB Human scFv
HPAB-0895-WJ-S(P) Human Anti-EGFR Recombinant Antibody (clone YZ-EGFR V2); scFv Fragment ELISA, WB Human scFv
HPAB-1519-FY-F(E) Human Anti-EGFR Recombinant Antibody (clone 15H8); scFv Fragment ELISA Human scFv
HPAB-2059-FY-S(P) Mouse Anti-EGFR Recombinant Antibody (clone L2-12B); scFv Fragment FC, ELISA Mouse scFv
HPAB-2060-FY-S(P) Mouse Anti-EGFR Recombinant Antibody (clone L3-11D); scFv Fragment FC, ELISA Mouse scFv

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

* Abbreviations
3D IHC3D Immunohistochemistry
ActivActivation
AgonistAgonist
ApopApoptosis
BABioassay
BIBioimaging
BlockBlocking
Cell ScreeningCell Screening
SeparationCell Separation
ChIPChromatin Immunoprecipitation
CMCDComplement Mediated Cell Depletion
CostimCostimulation
CytCytotoxicity
DepletionDepletion
DBDot Blot
EMElectron Microscopy
ELISAEnzyme-linked Immunosorbent Assay
ELISPOTEnzyme-linked Immunosorbent Spot
FCFlow Cytometry
FuncSFunctional Assay
GSGel Super Shift Assay
HAHemagglutination
REImmunohistology - Resin Sections
IPImmunoprecipitation
IRMAImmunoradiometric Assay
SHIn situ hybridization
InhibInhibition
ICFCIntracellular Staining for Flow Cytometry
KO/KD-WBKnockout/Knockdown target confirmation by Western Blot
CyTOF®Mass Cytometry
MeDIPMethylated DNA Immunoprecipitation
PGProteogenomics
RIRadial Immunodiffusion
RIARadioimmunoassay
StimStimulation
SPRSurface Plasmon Resonance
WBWestern Blot

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