Anti-EpCAM Immunocytokine, IL12-IgG (KS)-IL2 (ACFP-SH182)

This antibody-cytokine fusion protein was achieved by conjugating/fusing the Anti-EpCAM IgG to IL12 and IL2. It was expressed in CHO and purified with affinity chromatography. The Immunocytokines retains the ability to bind the EpCAM as well as the biological activity of IL12 and IL2. Cytokine activity was retained in constructs where one cytokine was fused at the carboxyl terminus of the H chain while the second cytokine was fused to the amino terminus of either the light (L) chain variable region, antigen binding of the antibody-cytokine fusion proteins could be maintained. These IL-12/IL-2 bi-functional fusion proteins were shown to induce extremely high levels of interferon-gamma (IFN-γ), similar to the synergy normally seen with the combined application of the individual cytokines. These bifunctional molecules were shown to have striking anti-tumor activity as either gene therapy or as an antibody cytokine (s) fusion protein, and may provide a useful approach to the treatment of cancer.      

Molecule Class
Species Reactivity
Expression Host
Related Disease
Solid tumor
Cytokines 1
Cytokines 1 Description
Interleukin 12 (IL-12) is an interleukin that is naturally produced by dendritic cells, macrophages, neutrophils, and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation. IL-12 is composed of a bundle of four alpha helices. It is a heterodimeric cytokine encoded by two separate genes, IL-12A (p35) and IL-12B (p40). The active heterodimer (referred to as 'p70'), and a homodimer of p40 are formed following protein synthesis.
Cytokine 1 Species
Cytokines 2
Cytokines 2 Description
Interleukin-2 (IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It is a protein that regulates the activities of white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. IL-2 is part of the body's natural response to microbial infection, and in discriminating between foreign ("non-self") and "self". IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes.
Cytokine 2 Species
This product can be used therapeutically to deliver biologically active ligands to a desired tissue.
Alternative Names
EPCAM; epithelial cell adhesion molecule; ESA; KSA; M4S1; MK-1; DIAR5; EGP-2
Entrez Gene ID
UniProt ID

For lab research use only, not for diagnostic, therapeutic or any in vivo human use.

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