Anti-Human IL4 Recombinant Antibody (TAB-221) (CAT#: TAB-221)
Recombinant monoclonal antibody to IL4. It is a humanized monoclonal antibody for the treatment of asthma.
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ELISA
Figure 1 Anti-Human IL4 Recombinant Antibody (TAB-221) in ELISA
ELISA analysis of TAB-221 was performed by coating with human IL4 protein (His tag). Then blocked with BSA and incubated with TAB-221. The HRP-conjugated goat anti-IgG was used as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
WB
Figure 2 Anti-Human IL4 Recombinant Antibody (TAB-221) in WB
Western blot analysis of TAB-221 was performed by loading human IL4 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-221 and a HRP goat anti-IgG (H+L) was used as the secondary antibody (1: 3000). Chemiluminescent detection was performed.
DB
Figure 3 Anti-Human IL4 Recombinant Antibody (TAB-221) in DB
Dot Blot analysis of TAB-221 was performed by coating with human IL4 protein (His tag).
TAB-221 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-IgG (H+L) secondary antibody (1: 3000)
NC: Negative control, PC: Positive control
Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Mouse
- Derivation
- Humanized (from mouse)
- Type
- IgG1 - kappa
- Specificity
- Tested positive against native human antigen.
- Species Reactivity
- Human
- Applications
- WB, ELISA, FC, IP, FuncS, IF, Neut
- Related Disease
- Allergy
Product Property
- Purity
- >95.0%, determined by analysis by RP-HPLC & analysis by SDS-PAGE.
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The IL4 antibody has been reported in applications of Inhib, Neut.
Target
- Alternative Names
- Pascolizumab;331243-22-2;SB-240683;IL4;interleukin 4;interleukin-4;B cell growth factor 1;B_cell stimulatory factor 1;BCGF 1;BCGF1;BSF1;IL 4;lymphocyte stimulatory factor 1;MGC79402;binetrakin;pitrakinra;IL-4;BSF-1;BCGF-1;
- Gene ID
- 3565
- UniProt ID
- P05112
Related Resources
Please refer to Pascolizumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Pascolizumab.
Asthma
Autoimmune Thyroid Disease
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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ScFv-Fc Chimera Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-102 | Mouse Anti-Il4 Recombinant Antibody (clone 11B11) | ELISA, FC | Mouse IgG |
| MOB-085LC | Mouse Anti-IL4 Recombinant Antibody (clone YC133) | ELISA, FuncS | Mouse IgG |
| FN-125CQ | Mouse Anti-IL4 Recombinant Antibody (clone CBL-1305) | FuncS, Neut, Inhib | Mouse IgG1 |
| ZG-0746F | Rat Anti-IL4 Antibody (clone AR.98) | FC, IF | Rat IgG1 |
| VS3-FY754 | Recombinant Rabbit Anti-IL4 Antibody (clone R08-2A7) | WB | Rabbit IgG |
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-221, RRID: AB_3111881)
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