Anti-Human IL4 Recombinant Antibody (TAB-221) (CAT#: TAB-221)
Recombinant monoclonal antibody to IL4. It is a humanized monoclonal antibody for the treatment of asthma.
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
Figure 1 Anti-Human IL4 Recombinant Antibody (TAB-221) in ELISA
ELISA analysis of TAB-221 was performed by coating with human IL4 protein (His tag). Then blocked with BSA and incubated with TAB-221. The HRP-conjugated goat anti-IgG was used as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 2 Anti-Human IL4 Recombinant Antibody (TAB-221) in WB
Western blot analysis of TAB-221 was performed by loading human IL4 protein onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-221 and a HRP goat anti-IgG (H+L) was used as the secondary antibody (1: 3000). Chemiluminescent detection was performed.
Figure 3 Anti-Human IL4 Recombinant Antibody (TAB-221) in DB
Dot Blot analysis of TAB-221 was performed by coating with human IL4 protein (His tag).
TAB-221 incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-IgG (H+L) secondary antibody (1: 3000)
NC: Negative control, PC: Positive control
Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Mouse
- Derivation
- Humanized (from mouse)
- Type
- IgG1 - kappa
- Specificity
- Tested positive against native human antigen.
- Species Reactivity
- Human
- Applications
- WB, ELISA, FC, IP, FuncS, IF, Neut
- Related Disease
- Allergy
Product Property
- Purity
- >95.0%, determined by analysis by RP-HPLC & analysis by SDS-PAGE.
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The IL4 antibody has been reported in applications of Inhib, Neut.
Target
- Alternative Names
- Pascolizumab;331243-22-2;SB-240683;IL4;interleukin 4;interleukin-4;B cell growth factor 1;B_cell stimulatory factor 1;BCGF 1;BCGF1;BSF1;IL 4;lymphocyte stimulatory factor 1;MGC79402;binetrakin;pitrakinra;IL-4;BSF-1;BCGF-1;
- Gene ID
- 3565
- UniProt ID
- P05112
Related Resources
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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DrMAB-102 | Mouse Anti-Il4 Recombinant Antibody (clone 11B11) | ELISA, FC | Mouse IgG |
MOB-085LC | Mouse Anti-IL4 Recombinant Antibody (clone YC133) | ELISA, FuncS | Mouse IgG |
FN-125CQ | Mouse Anti-IL4 Recombinant Antibody (clone CBL-1305) | FuncS, Neut, Inhib | Mouse IgG1 |
ZG-0746F | Rat Anti-IL4 Antibody (clone AR.98) | FC, IF | Rat IgG1 |
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-221, RRID: AB_3111881)
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For Research Use Only. Not For Clinical Use.
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