Human Anti-CD47 Recombinant Antibody (clone mIAP430) (CAT#: FAMAB-1068CQ)
This product is a recombinant human antibody that recognizes CD47. The antibody was purified by affinity chromatography. It can be used in Block, IF.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Figure 1 mAbs anti-CD47 (miaps) block fusion.
Alveolar macrophages were plated at 5 × 106/ml in 96-well plates and cultured in MEME supplemented with 5% human serum for 5 days. a, mAbs anti-CD47 miap 430, miap 450 and miap 470 (4 μg/ml) block fusion, but mAb anti-CD47 miap 400 fails to do so. Indeed, miap 400 is representative of six anti-CD47 mAbs that failed to block fusion (miaps 400, 410, 420, 440, 460, and 480).b, mAb anti-CD47 miap 450 blocks fusion in a concentration-dependent manner. Original magnification, ×100.
Han, X., Sterling, H., Chen, Y., Saginario, C., Brown, E. J., Frazier, W. A., ... & Vignery, A. (2000). CD47, a ligand for the macrophage fusion receptor, participates in macrophage multinucleation. Journal of Biological Chemistry, 275(48), 37984-37992.
Figure 2 Competitive binding between MFR and CD47.
a, alveolar macrophages were plated at 5 3 106/ml in 96-well plates, cultured inMEME supplemented with 5% human serum for 24 h, fixed with 4%paraformaldehyde, blocked in PBS plus 5% milk, and incubated for 1 h with increasing concentrations of mAb 10C4 or miap 430, then overnight with 20 nM GST-CD47e or GST-MFRev, respectively. GST fusion protein binding was detected using HRP-conjugated mouse anti-GST antibody.
Han, X., Sterling, H., Chen, Y., Saginario, C., Brown, E. J., Frazier, W. A., ... & Vignery, A. (2000). CD47, a ligand for the macrophage fusion receptor, participates in macrophage multinucleation. Journal of Biological Chemistry, 275(48), 37984-37992.
Specifications
- Immunogen
- Purified human placental CD47
- Host Species
- Human
- Type
- Human IgG1
- Specificity
- CD47
- Species Reactivity
- Human
- Clone
- mIAP430
- Applications
- Block, IF
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- CD47 Molecule; Antigenic Surface Determinant Protein OA3; CD47 Antigen (Rh-Related Antigen, Integrin-Associated Signal Transducer); Antigen Identified By Monoclonal Antibody 1D8; Leukocyte Surface Antigen CD47; Integrin Associated Protein; Rh-Related Antigen; CD47 Glycoprotein
- Gene ID
- 961
- UniProt ID
- Q08722
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone mIAP430"
See other products for "CD47"
Select a product category from the dropdown menu below to view related products.
Please select product type
Human Antibody Products
Humanized Antibody Products
Blocking Antibody Products
Neutralizing Antibody Products
Rabbit Monoclonal Antibody Products
Single Domain Antibody Products
IgG Antibody Products
Fab Antibody Products
ScFv Antibody Products
IgM Antibody Products
Non-Human Primate (NHP) Antibody Products
Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research
Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-278LC | Human Anti-CD47 Recombinant Antibody (TAB-278LC) | Block, FuncS | Human IgG, κ |
| TAB-279LC | Human Anti-CD47 Recombinant Antibody (TAB-279LC) | Block, FuncS | Human IgG, κ |
| TAB-278LC-S(P) | Human Anti-CD47 Recombinant Antibody; scFv Fragment (TAB-278LC-S(P)) | ELISA | Human scFv |
| TAB-278LC-F(E) | Human Anti-CD47 Recombinant Antibody; Fab Fragment (TAB-278LC-F(E)) | ELISA | Human Fab |
| TAB-279LC-F(E) | Human Anti-CD47 Recombinant Antibody; Fab Fragment (TAB-279LC-F(E)) | ELISA | Human Fab |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for FAMAB-1068CQ. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: FC, IP, ELISA, Neut, FuncS, IF, IHC
Application: FuncS, IF, Neut, ELISA, FC, IP, ICC
Application: ELISA, Neut, IF, IP, FC, FuncS
Application: FC, IHC, FuncS, Inhib, Cyt
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: FC, IP, ELISA, Neut, FuncS, IF, ICC
Application: Neut, ELISA, FuncS
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, FC
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
