Human Anti-CD47 Recombinant Antibody (clone mIAP430) (CAT#: FAMAB-1068CQ)

Figure 1 mAbs anti-CD47 (miaps) block fusion.

Alveolar macrophages were plated at 5 × 106/ml in 96-well plates and cultured in MEME supplemented with 5% human serum for 5 days. a, mAbs anti-CD47 miap 430, miap 450 and miap 470 (4 μg/ml) block fusion, but mAb anti-CD47 miap 400 fails to do so. Indeed, miap 400 is representative of six anti-CD47 mAbs that failed to block fusion (miaps 400, 410, 420, 440, 460, and 480).b, mAb anti-CD47 miap 450 blocks fusion in a concentration-dependent manner. Original magnification, ×100.

Han, X., Sterling, H., Chen, Y., Saginario, C., Brown, E. J., Frazier, W. A., ... & Vignery, A. (2000). CD47, a ligand for the macrophage fusion receptor, participates in macrophage multinucleation. Journal of Biological Chemistry, 275(48), 37984-37992.

Figure 2 Competitive binding between MFR and CD47.

a, alveolar macrophages were plated at 5 3 106/ml in 96-well plates, cultured inMEME supplemented with 5% human serum for 24 h, fixed with 4%paraformaldehyde, blocked in PBS plus 5% milk, and incubated for 1 h with increasing concentrations of mAb 10C4 or miap 430, then overnight with 20 nM GST-CD47e or GST-MFRev, respectively. GST fusion protein binding was detected using HRP-conjugated mouse anti-GST antibody.

Han, X., Sterling, H., Chen, Y., Saginario, C., Brown, E. J., Frazier, W. A., ... & Vignery, A. (2000). CD47, a ligand for the macrophage fusion receptor, participates in macrophage multinucleation. Journal of Biological Chemistry, 275(48), 37984-37992.