Afuco™ Anti-EGFR ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-020)

Name: Afuco™ Anti-EGFR ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-020)
SKU: AFC-TAB-020
Rating: 5 (1 reviews)

CAT#: AFC-TAB-020

This product is an ADCC enhanced antibody produced by our Afuco™ platform. Recombinant monoclonal antibody to Human EGFR. It is a fully human monoclonal antibody specific to the epidermal growth factor receptor (also known as EGF receptor, EGFR, ErbB-1 and HER1 in humans).

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cell junctions.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/144_E9_2_selected.jpg

Subcellular Location and Protein Expression Figure 2 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/143_E9_1_blue_red_green.jpg

Normal Tissue Figure 3 Colon

Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_3.jpg

Normal Tissue Figure 4 Liver

Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_4.jpg

Normal Tissue Figure 5 Kidney

Bowman's capsule
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Collecting ducts
Staining:Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Proximal tubules (cell body)
Staining:Medium
Intensity: Strong
Quantity: <25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_5.jpg

Normal Tissue Figure 6 Testis

Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_6_6.jpg

Normal Tissue Figure 7 Placenta

Cytotrophoblasts
Staining:High
Intensity: Strong
Quantity:>75%
Decidual cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Hofbauer cells
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - cell body
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_1_7.jpg

Normal Tissue Figure 8 Lymph node

Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_8.jpg

RNA Expression Figure 9 RNA cell line category: Cell line enriched (A-431)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000146648-EGFR

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Specifications

Immunogen
EGFR expressed on the surface of cells (A431 cells)

Host Species
Human

Derivation
Human

Type
ADCC enhanced antibody

Species Reactivity
Human

Applications
ELISA, IP, FC, FuncS, Neut, IF

Conjugate
Unconjugated

Trade name
Vectibix

Generic Name
Panitumumab

Related Disease
Colorectal cancers

Product Property

Purity
>95% by SDS-PAGE

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
EGFR; epidermal growth factor receptor; epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog), ERBB; ERBB1;

Gene ID
1956

UniProt ID
P00533

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Related Diseases

Bladder Cancer

Non-small Cell Lung Cancer

Pancreatic Cancer

Hepatocellular Carcinoma

Cushing Syndrome

Related Signaling Pathways

JAK-STAT Signaling Pathway

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - AFC-TAB-020.pdf

MSDS

MSDS - AFC-TAB-020.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

See other products for "Panitumumab"

Anti-Human EGFR Recombinant Antibody (Panitumumab)
Recombinant monoclonal antibody to Human EGFR. Panitumumab (INN), formerly ABX-EGF, is a fully human monoclonal antibody specific to the epidermal growth factor receptor (also known as EGF receptor, EGFR, ErbB-1 and HER1 in humans).

DrugMonitor™ Anti-Panitumumab Antibody (VS-1224-YC884)
Panitumumab is a recombinant humanized monoclonal antibody used in the treatment of EGFR-expressing metastatic colorectal carcinoma that is refractory to chemotherapy regimens containing fluoropyrimidine, oxaliplatin, and irinotecan. The DrugMonitor™ Anti-Panitumumab Antibody (VS-1224-YC884) is an anti-drug antibody (ADA) against Panitumumab. This drug-based antibody is raised in mice immunized with the Panitumumab. The anti-Panitumumab antibody may be used in ELISA, pharmacokinetics (PK), and pharmacodynamics (PD) analyses, or serves as a reference standard in ADA assays. It also is an excellent tool for therapeutic drug monitoring, allowing to evaluate the drug efficacy and determine the drug concentration of the Panitumumab in samples.

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TAB-302MZ-S(P)	Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-302MZ-S(P))	ELISA	Mouse scFv
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CAT	Product Name	Application	Type
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PABW-088	Mouse Anti-EGFR Recombinant Antibody (clone 7A7)	ELISA, WB	Mouse IgG
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HPAB-0137-YJ	Human Anti-EGFR Recombinant Antibody (HPAB-0137-YJ)	ELISA, FC, Inhib, FuncS	Human IgG1
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CAT	Product Name	Application	Type
PFBL-459	Human Anti-EGFR Recombinant Antibody (clone C225); Fab Fragment	FC	Human Fab
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TAB-278MZ-F(E)	Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-278MZ-F(E))	ELISA, WB	Human Fab
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TAB-283MZ-F(E)	Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-283MZ-F(E))	ELISA	Human Fab
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Gly-055LC	Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated)	ELISA	Chimeric antibody (mouse/human)
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CAT	Product Name	Application	Type
Gly-055LC-1	Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated)	ELISA	Chimeric antibody (mouse/human)

CAT	Product Name	Application	Type
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CAT	Product Name	Application	Type
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CAT	Product Name	Application
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MOR-4570	Hi-Affi™ Rabbit Anti-EGFR Recombinant Antibody (clone TH82DS)	ELISA	Rabbit IgG
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CAT	Product Name	Application	Type
HPAB-0010-YC-S(P)	Mouse Anti-EGFR Recombinant Antibody (clone L211C); scFv Fragment	ELISA, FC	Mouse scFv
HPAB-0011-YC-S(P)	Human Anti-EGFR Recombinant Antibody (clone Pep 1); scFv Fragment	FC	Human scFv
HPAB-0012-YC-S(P)	Human Anti-EGFR Recombinant Antibody (clone Pep 2); scFv Fragment	FC	Human scFv
HPAB-0013-YC-S(P)	Human Anti-EGFR Recombinant Antibody (clone Pep 3); scFv Fragment	FC	Human scFv
HPAB-0014-YC-S(P)	Human Anti-EGFR Recombinant Antibody (clone Pep 4); scFv Fragment	FC	Human scFv

CAT	Product Name	Application	Type
AFC-TAB-165	Afuco™ Anti-EGFR ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-165)	Neut, ELISA, IF, IP, FuncS, FC	ADCC enhanced antibody
AFC-TAB-464CQ	Afuco™ Anti-EGFR ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-464CQ)	ELISA, IHC, FC, IP, IF, FuncS	ADCC enhanced antibody
AFC-TAB-003	Afuco™ Anti-EGFR ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-003)	IF, IP, Neut, FuncS, ELISA, FC	ADCC enhanced antibody
AFC-TAB-040	Afuco™ Anti-EGFR ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-040)	ELISA, FC, IP, FuncS, IF, Neut	ADCC enhanced antibody
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CAT	Product Name	Application	Type
VS-1024-XY177	Mouse Anti-NHP EGFR Recombinant Antibody (clone 225)	IF, IP	Mouse IgG1

CAT	Product Name	Application	Type
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CAT	Product Name	Application	Type
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CAT	Product Name	Application
VS-0325-XY735	Anti-EGFR Immunohistochemistry Kit	IHC
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VS-0525-XY2182	Anti-Human EGFR Immunohistochemistry Kit	IHC

CAT	Product Name	Application	Type
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VS-0525-YC65	Recombinant Anti-EGFR (AA 269-278 x AA 526-535) Biparatopic Antibody, Tandem scFv (Clone Pep 2 x Clone Pep 3)	FC	Tandem scFv
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