Mouse Anti-EGFR Recombinant Antibody (clone EGFR) (CAT#: HPAB-0727-CN)

The mAb A13 specifically bound to a variety of tumor cells and human placenta tissues expressing EGFR. A13 efficiently inhibited both EGF-dependant EGFR tyrosine phosphorylation in cervical and breast tumor cells and also in vitro colony formation of EGFR-overexpressing lung tumors.

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SDS-PAGE

Figure 1 Mouse Anti-EGFR Recombinant Antibody (HPAB-0727-CN) in SDS-PAGE

Figure 1 Mouse Anti-EGFR Recombinant Antibody (HPAB-0727-CN) in SDS-PAGE

Lane 2: Non-reduced mAb
Lane 1: Reduced mAb

SEC-HPLC

Figure 2 Mouse Anti-EGFR Recombinant Antibody (HPAB-0727-CN) in HPLC

Figure 2 Mouse Anti-EGFR Recombinant Antibody (HPAB-0727-CN) in HPLC

The purity of HPAB-0727-CN was greater than 95% as determined by SEC-HPLC.

ELISA

Figure 3 Mouse Anti-EGFR Recombinant Antibody (HPAB-0727-CN) in ELISA

Figure 3 Mouse Anti-EGFR Recombinant Antibody (HPAB-0727-CN) in ELISA

ELISA analysis of HPAB-0727-CN was performed by coating with Human EGFR Protein.
The secondary antibody: HRP-Anti-Mouse IgG (H+L)

WB

Figure 4 Mouse Anti-EGFR Recombinant Antibody (HPAB-0727-CN) in WB

Figure 4 Mouse Anti-EGFR Recombinant Antibody (HPAB-0727-CN) in WB

Western blot analysis of HPAB-0727-CN was performed by loading Human EGFR Protein.
HPAB-0727-CN incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Mouse IgG (H+L)
Lane1: Reduced antigen (0.1 μg)
Lane2: Reduced antigen (0.3 μg)
Lane2: Reduced antigen (0.6 μg)


Specifications

  • Immunogen
  • Human cervical epidermal carcinoma A431 cells
  • Host Species
  • Mouse
  • Type
  • Mouse IgG1, κ
  • Specificity
  • Human EGFR
  • Species Reactivity
  • Human, Mouse
  • Clone
  • EGFR
  • Applications
  • ELISA, FC, IHC, Neut
  • Related Disease
  • Epithelial solid tumors including lung, colon, breast, prostate, brain, head, neck, ovarian, renal carcinoma

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

  • Alternative Names
  • Epidermal Growth Factor Receptor; Receptor Tyrosine-Protein Kinase ErbB-1; Erb-B2 Receptor Tyrosine Kinase 1; Proto-Oncogene C-ErbB-1; EC 2.7.10.1; ERBB1; ERBB; HER1; Epidermal Growth Factor Receptor (Avian Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog); Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog (Avian);

Related Resources

  • Related Diseases
  • Related Signaling Pathways

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "Clone EGFR"

See other products for "EGFR"

Fc Glycosylation

High-mannose Glycoform

CAT Product Name Application Type
Gly-055LC-1 Recombinant Anti-Human EGFR Antibody (Fc glycosylation/High-mannose glycosylated) ELISA Chimeric antibody (mouse/human)

Deglycosylated Antibody (Non-glycosylated IgGs)

CAT Product Name Application Type
Gly-167LC Recombinant Anti-Human EGFR Antibody (Non-glycosylated) ELISA Human antibody

Chicken IgY Antibody

CAT Product Name Application Type
BRD-0183MZ Chicken Anti-EGFR Polyclonal IgY WB Chicken antibody
BRD-0668MZ Chicken Anti-EGFR Polyclonal IgY WB Chicken antibody

Neutralizing Antibody

ADCC Enhanced Antibody

CAT Product Name Application Type
AFC-TAB-165 Afuco™ Anti-EGFR ADCC Recombinant Antibody (Matuzumab), ADCC Enhanced Neut, ELISA, IF, IP, FuncS, FC ADCC enhanced antibody
AFC-TAB-464CQ Afuco™ Anti-EGFR ADCC Recombinant Antibody (Tomuzotuximab), ADCC Enhanced ELISA, IHC, FC, IP, IF, FuncS ADCC enhanced antibody
AFC-TAB-003 Afuco™ Anti-EGFR ADCC Recombinant Antibody (Cetuximab), ADCC Enhanced IF, IP, Neut, FuncS, ELISA, FC ADCC enhanced antibody
AFC-TAB-040 Afuco™ Anti-EGFR ADCC Recombinant Antibody (Zalutumumab), ADCC Enhanced ELISA, FC, IP, FuncS, IF, Neut ADCC enhanced antibody
AFC-TAB-119 Afuco™ Anti-EGFR ADCC Recombinant Antibody (Necitumumab), ADCC Enhanced FC, IP, ELISA, Neut, FuncS, IF ADCC enhanced antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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