Mouse Anti-EGFR Recombinant Antibody (clone EGFR) (CAT#: HPAB-0727-CN)
The mAb A13 specifically bound to a variety of tumor cells and human placenta tissues expressing EGFR. A13 efficiently inhibited both EGF-dependant EGFR tyrosine phosphorylation in cervical and breast tumor cells and also in vitro colony formation of EGFR-overexpressing lung tumors.
Specific Inquiry
SDS-PAGE
Figure 1 Mouse Anti-EGFR Recombinant Antibody (HPAB-0727-CN) in SDS-PAGE
Lane 2: Non-reduced mAb
Lane 1: Reduced mAb
SEC-HPLC
Figure 2 Mouse Anti-EGFR Recombinant Antibody (HPAB-0727-CN) in HPLC
The purity of HPAB-0727-CN was greater than 95% as determined by SEC-HPLC.
ELISA
Figure 3 Mouse Anti-EGFR Recombinant Antibody (HPAB-0727-CN) in ELISA
ELISA analysis of HPAB-0727-CN was performed by coating with Human EGFR Protein.
The secondary antibody: HRP-Anti-Mouse IgG (H+L)
WB
Figure 4 Mouse Anti-EGFR Recombinant Antibody (HPAB-0727-CN) in WB
Western blot analysis of HPAB-0727-CN was performed by loading Human EGFR Protein.
HPAB-0727-CN incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Mouse IgG (H+L)
Lane1: Reduced antigen (0.1 μg)
Lane2: Reduced antigen (0.3 μg)
Lane2: Reduced antigen (0.6 μg)
Specifications
- Immunogen
- Human cervical epidermal carcinoma A431 cells
- Host Species
- Mouse
- Type
- Mouse IgG1, κ
- Specificity
- Human EGFR
- Species Reactivity
- Human, Mouse
- Clone
- EGFR
- Applications
- ELISA, FC, IHC, Neut
- Related Disease
- Epithelial solid tumors including lung, colon, breast, prostate, brain, head, neck, ovarian, renal carcinoma
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Epidermal Growth Factor Receptor; Receptor Tyrosine-Protein Kinase ErbB-1; Erb-B2 Receptor Tyrosine Kinase 1; Proto-Oncogene C-ErbB-1; EC 2.7.10.1; ERBB1; ERBB; HER1; Epidermal Growth Factor Receptor (Avian Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog); Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog (Avian);
- Gene ID
- 1956
- UniProt ID
- P00533
Related Resources
Bladder Cancer
Non-small Cell Lung Cancer
Pancreatic Cancer
Hepatocellular Carcinoma
Cushing Syndrome
JAK-STAT Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Chimeric Antibody
Human Antibody
Recombinant Antibody
Single-domain Antibody
Mouse Antibody
Humanized Antibody
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High-mannose Glycoform
Deglycosylated Antibody (Non-glycosylated IgGs)
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Neutralizing Antibody
Rabbit Monoclonal Antibody
Fab Fragment Antibody
ADCC Enhanced Antibody
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-750 | Anti-EGFR/HER1 Recombinant Antibody (Futuximab/Zatuximab) | Neut, ELISA, IF, IP, FuncS, FC, WB | IgG1 - kappa |
| TAB-003 | Anti-Human EGFR Recombinant Antibody (Cetuximab) | IF, IP, Neut, FuncS, ELISA, FC, ICC | IgG1 - kappa |
| TAB-H49 | Anti-Human EGFR Recombinant Antibody (Modotuximab) | FuncS, IF, Neut, ELISA, FC, IP, IHC | IgG1 - kappa |
| TAB-308MZ-S(P) | Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-308MZ-S(P)) | ELISA, FC | Mouse scFv |
| TAB-308MZ-F(E) | Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-308MZ-F(E)) | ELISA, FC | Chimeric antibody (mouse/human) |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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