Does this antibody compete with EGF for binding?

Yes, the monoclonal antibody 425 (MAb 425) binds to the external domain of the human EGFR and has been shown to inhibit the binding of epidermal growth factor (EGF) at both low and high affinity EGFR sites. This blocking capability makes it useful for signal transduction research.

What applications can I use this antibody for?

This Anti-EGFR Recombinant Antibody is versatile and validated for multiple applications, including ELISA, Flow Cytometry (FC), Block, and Cytotoxicity assays (Cyt). It was found to mediate tumor cytotoxicity and suppress tumor cell growth in vitro, providing a robust tool for cancer research.

Anti-EGFR Recombinant Antibody (clone MAb 425)

CAT#: VS-0524-YC33

The monoclonal antibody 425 (MAb 425) was raised against the human A431 carcinoma cell line and binds to the external domain of the human epidermal growth factor receptor (EGFR). The antibody inhibited the binding of epidermal growth factor (EGF) at both low and high affinity EGFR sites. MAb 425 was found to mediate tumor cytotoxicity and to suppress tumor cell growth of epidermoid and colorectal carcinoma-derived cell lines in vitro.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cell junctions.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/144_E9_2_selected.jpg

Subcellular Location and Protein Expression Figure 2 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/143_E9_1_blue_red_green.jpg

Normal Tissue Figure 3 Colon

Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_3.jpg

Normal Tissue Figure 4 Liver

Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_4.jpg

Normal Tissue Figure 5 Kidney

Bowman's capsule
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Collecting ducts
Staining:Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Proximal tubules (cell body)
Staining:Medium
Intensity: Strong
Quantity: <25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_5.jpg

Normal Tissue Figure 6 Testis

Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_6_6.jpg

Normal Tissue Figure 7 Placenta

Cytotrophoblasts
Staining:High
Intensity: Strong
Quantity:>75%
Decidual cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Hofbauer cells
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - cell body
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_1_7.jpg

Normal Tissue Figure 8 Lymph node

Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_8.jpg

RNA Expression Figure 9 RNA cell line category: Cell line enriched (A-431)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000146648-EGFR

❮

❯

Sub Cat	Host Animal	Derivation	Type	Purification	Size	Conjugation	Endotoxin	Purity	Fc Engineering
VS-0524-YC48	Mouse	Hybridoma	Mouse IgG	Protein G purified
VS-0524-YC34	Human	Chimeric (mouse/human)	Human IgG	Protein A purified

Specifications

Immunogen
The human A431 carcinoma cell line

Specificity
Human Epidermal growth factor receptor (EGFR)

Species Reactivity
Human

Clone
MAb 425

Applications
ELISA, FC, Block, Cyt

Product Property

Purity
>95%

Buffer
PBS, pH 7.4

Preservative
No preservative

Storage
Centrifuge briefly prior to opening vial. Store at 4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
Epidermal Growth Factor Receptor; Receptor Tyrosine-Protein Kinase ErbB-1; Erb-B2 Receptor Tyrosine Kinase 1; Proto-Oncogene C-ErbB-1; EC 2.7.10.1; ERBB1; ERBB; HER1; Epidermal Growth Factor Receptor (Avian Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog); Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog (Avian)

Gene ID
1956

UniProt ID
P00533

Long Name
Epidermal growth Factor receptor

Cellular Localization
Cell membrane, Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane, Nucleus, Secreted

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Breast cancer biomarkers at key points during disease progression

Inhibits EGF Binding Effectively

We used the MAb 425 clone to investigate EGFR signaling. This antibody successfully inhibited the binding of epidermal growth factor to the receptor in our cell lines. It also mediated tumor cytotoxicity in our in vitro models, confirming the functional activity described in the product data.

Versatile EGFR Analysis Tool

This recombinant antibody proved to be a versatile tool for our lab, working well in ELISA, Flow Cytometry, and blocking assays. It specifically binds to the external domain of human EGFR. The consistency of the recombinant production allows us to compare data across different experiments with high confidence.

Q&As

Does this antibody compete with EGF for binding?

A: Yes, the monoclonal antibody 425 (MAb 425) binds to the external domain of the human EGFR and has been shown to inhibit the binding of epidermal growth factor (EGF) at both low and high affinity EGFR sites. This blocking capability makes it useful for signal transduction research.
What applications can I use this antibody for?

A: This Anti-EGFR Recombinant Antibody is versatile and validated for multiple applications, including ELISA, Flow Cytometry (FC), Block, and Cytotoxicity assays (Cyt). It was found to mediate tumor cytotoxicity and suppress tumor cell growth in vitro, providing a robust tool for cancer research.

View the frequently asked questions answered by Creative Biolabs Support.

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Related Diseases

Bladder Cancer

Non-small Cell Lung Cancer

Pancreatic Cancer

Hepatocellular Carcinoma

Cushing Syndrome

Related Signaling Pathways

JAK-STAT Signaling Pathway

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS-0524-YC33.pdf

MSDS

MSDS - VS-0524-YC33.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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