Recombinant Camelid Anti-EGFR Single Domain Antibody

ELISA

Figure 1 Reactivity of purified Anti EGFR-SdAb and mAb C225 with A431 cell lysate.

The OD values related to reactivity of SdAb and mAb C225 with A431 cell lysate were plotted against different concentration of SdAb and mAb C225 (µg/ml).

Khosroshahi, S. A., Farajnia, S., Ghiamirad, M., Tanomand, A., Veisi, K., Rahbarnia, L., & Akbari, B. (2016). Development and evaluation of a single domain antibody against human epidermal growth factor receptor (EGFR). Protein expression and purification, 120, 59-64.

WB

Figure 2 Western blotting analysis of reactivity of the recombinant SdAb with A431 cells.

Khosroshahi, S. A., Farajnia, S., Ghiamirad, M., Tanomand, A., Veisi, K., Rahbarnia, L., & Akbari, B. (2016). Development and evaluation of a single domain antibody against human epidermal growth factor receptor (EGFR). Protein expression and purification, 120, 59-64.

ELISA

Figure 3 ELISA to detect A431 specific antibody titers in llama serum.

Subcellular Location and Protein Expression

Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cell junctions.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/144_E9_2_selected.jpg

Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/143_E9_1_blue_red_green.jpg

Normal Tissue

Figure 3 Colon
(Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_3.jpg

Figure 4 Liver
(Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_4.jpg

Figure 5 Kidney
(Bowman's capsule
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Collecting ducts
Staining:Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining:Medium
Intensity: Strong
Quantity: <25%
Proximal tubules (cell body)
Staining:Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_9_5.jpg

Figure 6 Testis
(Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Pachytene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Round or early spermatids
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_6_6.jpg

Figure 7 Placenta
(Cytotrophoblasts
Staining:High
Intensity: Strong
Quantity:>75%
Decidual cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Hofbauer cells
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - cell body
Staining:High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:High
Intensity: Strong)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_1_7.jpg

Figure 8 Lymph node
(Non-germinal center cells
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/18530/41191_A_7_8.jpg

RNA Expression

Figure 9 RNA cell line category: Cell line enriched (A-431)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000146648-EGFR

Datasheet

MSDS

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

CAT	Product Name	Application	Type
TAB-750	Anti-EGFR/HER1 Recombinant Antibody (Futuximab/Zatuximab)	Neut, ELISA, IF, IP, FuncS, FC, WB	IgG1 - kappa
TAB-003	Anti-Human EGFR Recombinant Antibody (Cetuximab)	IF, IP, Neut, FuncS, ELISA, FC, ICC	IgG1 - kappa
TAB-0225CL-S(P)	Human Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-0225CL-S(P))	Block, Inhib, FuncS, Apop, In vivo	Human scFv
TAB-272MZ	Human Anti-EGFR Recombinant Antibody (TAB-272MZ)	FuncS	Chimeric (mouse/human) IgG
TAB-308MZ-F(E)	Human Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-308MZ-F(E))	ELISA, FC	Chimeric antibody (mouse/human)

CAT	Product Name	Application	Type
TAB-040	Anti-Human EGFR Recombinant Antibody (Zalutumumab)	ELISA, FC, IP, FuncS, IF, Neut, ICC	IgG1 - kappa
TAB-119	Anti-Human EGFR Recombinant Antibody (Necitumumab)	FC, IP, ELISA, Neut, FuncS, IF, WB	IgG1 - kappa
PABX-053	Recombinant Human Anti-EGFR Antibody (DL11)	WB, ELISA, FuncS	IgG
PABX-052-S (P)	Recombinant Human Anti-EGFR Antibody scFv Fragment (Nimotuzumab)	WB, ELISA, FuncS	scFv
PABX-053-S (P)	Recombinant Human Anti-EGFR Antibody scFv Fragment (DL11)	WB, ELISA, FuncS	scFv

CAT	Product Name	Application	Type
TAB-753	Anti-EGFR Recombinant Antibody (Imgatuzumab)	Neut, ELISA, IF, IP, FuncS, FC, WB	IgG1 - kappa
TAB-228CL	Anti-Human EGFR Recombinant Antibody (ABT-806)	WB, IHC	Antibody
TAB-274MZ	Human Anti-EGFR Recombinant Antibody (TAB-274MZ)	FC	Humanized IgG
TAB-274MZ-S(P)	Human Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-274MZ-S(P))	FC	Humanized scFv
TAB-300MZ-S(P)	Human Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-300MZ-S(P))	ELISA	Humanized scFv

CAT	Product Name	Application	Type
PNBL-016	Recombinant Anti-Human EGFR VHH Single Domain Antibody (7D12)	WB, ELISA	Llama VHH
PNBL-017	Recombinant Anti-Human EGFR VHH Single Domain Antibody (9G8)	FuncS, ELISA, IF	Llama VHH
PNBL-018	Recombinant Anti-Human EGFR VHH Single Domain Antibody (EgA1)	FuncS, SPR	Llama VHH
HPAB-AP881-YC	Recombinant Llama Anti-EGFR Single Domain Antibody (VHH122)	ELISA, FC, IP, FuncS	Llama VHH
HPAB-AP882-YC	Recombinant Llama Anti-EGFR Single Domain Antibody (VHH205)	ELISA	Llama VHH

CAT	Product Name	Application	Type
TAB-0565CL-S(P)	Mouse Anti-EGFR Recombinant Antibody; scFv Fragment (TAB-0565CL-S(P))	ELISA	Mouse scFv
TAB-0564CL-F(E)	Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-0564CL-F(E))	ELISA	Mouse Fab
TAB-0565CL-F(E)	Mouse Anti-EGFR Recombinant Antibody; Fab Fragment (TAB-0565CL-F(E))	ELISA	Mouse Fab
TAB-316MZ-F(E)	Anti-Human EGFR Recombinant Antibody Fab Fragment (07D06)	WB, MTT assay
TAB-317MZ-F(E)	Anti-Human EGFR Recombinant Antibody Fab Fragment (12D03)	WB, MTT assay

Recombinant Camelid Anti-EGFR Single Domain Antibody (CAT#: HPAB-1027WJ)

Subcellular Location and Protein Expression

Normal Tissue

RNA Expression

Specifications

Applications

Target

Related Resources

Product Notes

Downloads

See other products for "EGFR"

Chimeric Antibody

Human Antibody

Humanized Antibody

Single-domain Antibody

Mouse Antibody

Fc Glycosylation

High-mannose Glycoform

Deglycosylated Antibody (Non-glycosylated IgGs)

Chicken IgY Antibody

MHC Tetramer for Cancer

Neutralizing Antibody

Rabbit Monoclonal Antibody

Recombinant Antibody

scFv Fragment Antibody

Fab Fragment Antibody

ADCC Enhanced Antibody

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