Rabbit Anti-IL15 Recombinant Antibody (clone R78-4W-8) (CAT#: VS3-FY2192)
This rabbit recombinant monoclonal antibody demonstrates a notable level of specificity and affinity towards human IL15 in western blotting at a dilution range of 1/500 to 1/1000. The calculated molecular weight of the protein is 18 kDa, while the observed molecular weight stands at 13 kDa.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A549
(Immunofluorescent staining of human cell line A549 shows localization to nuclear speckles & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37738/if_selected.jpg
(Immunofluorescent staining of human cell line A549 shows localization to nuclear speckles & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37738/if_selected.jpg
Figure 2 IHC staining of human lymph node
(Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in reaction center cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4457/ihc_selected.jpg
(Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in reaction center cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4457/ihc_selected.jpg
Figure 3 IF staining of human cell line HeLa
(Immunofluorescent staining of human cell line HeLa shows localization to nuclear speckles & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37738/785_B10_1_red_green.jpg
(Immunofluorescent staining of human cell line HeLa shows localization to nuclear speckles & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37738/785_B10_1_red_green.jpg
Figure 4 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37738/783_B10_1_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37738/783_B10_1_red_green.jpg
Figure 5 Cerebral cortex
(Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4457/15421_B_8_5.jpg
(Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4457/15421_B_8_5.jpg
Figure 6 Lung
(Macrophages
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4457/15421_A_1_4.jpg
(Macrophages
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4457/15421_A_1_4.jpg
Figure 7 Colon
(Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4457/15421_A_8_3.jpg
(Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4457/15421_A_8_3.jpg
Figure 8 Testis
(Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4457/15421_A_6_6.jpg
(Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4457/15421_A_6_6.jpg
Figure 9 Lymph node
(Germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear
Non-germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4457/15421_A_9_8.jpg
(Germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear
Non-germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4457/15421_A_9_8.jpg
Figure 10 RNA cell line category: Cell line enhanced (HDLM-2, Karpas-707)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000164136-IL15
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000164136-IL15
Figure 10 RNA cell line category: Cell line enhanced (HDLM-2, Karpas-707)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000164136-IL15
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000164136-IL15
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human IL15
- Species Reactivity
- Human
- Clone
- R78-4W-8
- Applications
- Western Blot
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 18kDa, while the observed molecular weight stands at 13kDa.
- Related Disease
- Severe Combined Immunodeficiency, X-Linked and Primary Cutaneous T-Cell Non-Hodgkin Lymphoma
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
- Alternative Names
- Interleukin 15
- Gene ID
- 3600
- UniProt ID
- P40933
- Sequence Similarities
- Belongs to the IL-15/IL-21 family.
- Cellular Localization
- Cytoplasm, Nucleus, Secreted
- Post Translation Modifications
- Glycosylation at Asn127 (NX_P40933 [NX_P40933-1])
Modification sites at PhosphoSitePlus (P40933)
Glycosylation from GlyGen (P40933) 1 site
- Protein Refseq
- NP_000576.1; NP_751915.1
- Function
- Cytokine that plays a major role in the development of inflammatory and protective immune responses to microbial invaders and parasites by modulating immune cells of both the innate and adaptive immune systems.
Stimulates the proliferation of natural killer cells, T-cells and B-cells and promotes the secretion of several cytokines.
Recommended Products
Isotype Control
Secondary Antibody
Related Resources
Rheumatoid Arthritis
TNF Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "IL15"
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1254z-F(E) | Recombinant Anti-Human IL15 Antibody Fab Fragment | IF, FACS, FuncS | Fab |
| HPAB-S0072-YC-F(E) | Human Anti-IL15 Recombinant Antibody (clone 4H10); Fab Fragment | ELISA | Human Fab |
| HPAB-S0073-YC-F(E) | Human Anti-IL15 Recombinant Antibody (clone 1H3); Fab Fragment | ELISA | Human Fab |
| HPAB-S0074-YC-F(E) | Human Anti-IL15 Recombinant Antibody (clone 3C1); Fab Fragment | ELISA | Human Fab |
| HPAB-S0075-YC-F(E) | Human Anti-IL15 Recombinant Antibody (clone 8C5); Fab Fragment | ELISA | Human Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1254z-S(P) | Recombinant Anti-Human IL15 Antibody scFv Fragment | WB, IF, FuncS | scFv |
| HPAB-0238-YC-S(P) | Human Anti-IL15 Recombinant Antibody; scFv Fragment (HPAB-0238-YC-S(P)) | ELISA, FC, FuncS | Human scFv |
| HPAB-S0073-YC-S(P) | Human Anti-IL15 Recombinant Antibody (clone 1H3); scFv Fragment | ELISA | Human scFv |
| HPAB-S0074-YC-S(P) | Human Anti-IL15 Recombinant Antibody (clone 3C1); scFv Fragment | ELISA | Human scFv |
| HPAB-S0075-YC-S(P) | Human Anti-IL15 Recombinant Antibody (clone 8C5); scFv Fragment | ELISA | Human scFv |
Rat Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-398CL | Human Anti-IL15 Recombinant Antibody (TAB-398CL) | ELISA, WB | Human IgG |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-382CL | Afuco™ Anti-IL15 Recombinant Antibody (AFC-382CL), ADCC Enhanced | ELISA, WB | Human IgG1 |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABZ-081 | Human Anti-IL15 Recombinant Antibody (clone DISC0280) | Neut | Human IgG |
| MOB-0784CT | Recombinant Mouse anti-Human IL15 Monoclonal antibody (C-F30) | Neut | |
| HPAB-0238-YC | Human Anti-IL15 Recombinant Antibody (HPAB-0238-YC) | ELISA, FC, Inhib, FuncS | Human IgG |
| HPAB-S0072-YC | Human Anti-IL15 Recombinant Antibody (clone 4H10) | ELISA | Human IgG |
| HPAB-S0073-YC | Human Anti-IL15 Recombinant Antibody (clone 1H3) | ELISA | Human IgG |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0234CL | Human Anti-IL15 Recombinant Antibody (TAB-0234CL) | ELISA | Human IgG |
| TAB-0235CL | Human Anti-IL15 Recombinant Antibody (TAB-0235CL) | ELISA | Human IgG |
| TAB-0236CL | Human Anti-IL15 Recombinant Antibody (TAB-0236CL) | ELISA | Human IgG |
| TAB-0237CL | Human Anti-IL15 Recombinant Antibody (TAB-0237CL) | ELISA | Human IgG |
| TAB-0238CL | Human Anti-IL15 Recombinant Antibody (TAB-0238CL) | ELISA | Human IgG |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-075ZJ | Anti-Human IL-15 Recombinant Antibody (DISC0100) | ELISA | Human antibody |
| TAB-075ZJ-S(P) | Anti-Human IL-15 Recombinant Antibody scFv Fragment (DISC0100) | ELISA | Human antibody |
| TAB-076ZJ-S(P) | Anti-Human IL-15 Recombinant Antibody scFv Fragment (DISC0280) | ELISA | Human antibody |
| TAB-075ZJ-F(E) | Anti-Human IL-15 Recombinant Antibody Fab Fragment (DISC0100) | ELISA | Human antibody |
| TAB-076ZJ-F(E) | Anti-Human IL-15 Recombinant Antibody Fab Fragment (DISC0280) | ELISA | Human antibody |
Fc Glycosylation
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-198LC | Recombinant Anti-Human IL15 Antibody (Fc glycosylation/High-mannose glycosylated) | ELISA | Human antibody |
High-mannose Glycoform
| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-198LC-1 | Recombinant Anti-Human IL15 Antibody (Fc glycosylation/High-mannose glycosylated) | ELISA | Human antibody |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1191CQ | Mouse Anti-IL15 Recombinant Antibody (clone 34593.11) | ELISA, Neut, WB | Mouse IgG1 |
| NEUT-1192CQ | Mouse Anti-IL15 Recombinant Antibody (clone MM0368-13S34) | WB, Neut | Mouse IgG1 |
| NEUT-1193CQ | Rat Anti-IL15 Recombinant Antibody (clone AIO.3) | Neut | Rat IgG2a, κ |
| NEUT-1195CQ | Mouse Anti-IL15 Recombinant Antibody (clone CBL096) | Neut, WB | Mouse IgG1 |
| NEUT-1196CQ | Mouse Anti-IL15 Recombinant Antibody (clone CBL457) | WB, CyTOF, ICFC, Neut | Mouse IgG1 |
Blocking Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1194CQ | Mouse Anti-IL15 Recombinant Antibody (clone B-E29) | Block | Mouse IgG1 |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1792 | Rabbit Anti-IL15 Recombinant Antibody (clone DS1792AB) | WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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