Recombinant Rabbit Anti-IL15 Capture Antibody (clone R02-5K7) (CAT#: VS3-WK1716)
This antibody is a rabbit recombinant antibody against human IL15. It was developed by immunization of rabbit with recombinant human IL15 protein and then was produced as a recombinant antibody. The isotype of this product is rabbit IgG. The sandwich ELISA was established using matched antibody pair R02-5K7 and R05-5W7. The clone R02-5K7 can be used as a capture antibody in ELISA analysis to identify human IL15.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A549
(Immunofluorescent staining of human cell line A549 shows localization to nuclear speckles & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37738/if_selected.jpg
(Immunofluorescent staining of human cell line A549 shows localization to nuclear speckles & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37738/if_selected.jpg
Figure 2 IHC staining of human lymph node
(Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in reaction center cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4457/ihc_selected.jpg
(Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in reaction center cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4457/ihc_selected.jpg
Figure 3 IF staining of human cell line HeLa
(Immunofluorescent staining of human cell line HeLa shows localization to nuclear speckles & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37738/785_B10_1_red_green.jpg
(Immunofluorescent staining of human cell line HeLa shows localization to nuclear speckles & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37738/785_B10_1_red_green.jpg
Figure 4 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37738/783_B10_1_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/37738/783_B10_1_red_green.jpg
Figure 5 Cerebral cortex
(Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4457/15421_B_8_5.jpg
(Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4457/15421_B_8_5.jpg
Figure 6 Lung
(Macrophages
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4457/15421_A_1_4.jpg
(Macrophages
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4457/15421_A_1_4.jpg
Figure 7 Colon
(Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4457/15421_A_8_3.jpg
(Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4457/15421_A_8_3.jpg
Figure 8 Testis
(Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4457/15421_A_6_6.jpg
(Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4457/15421_A_6_6.jpg
Figure 9 Lymph node
(Germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear
Non-germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4457/15421_A_9_8.jpg
(Germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear
Non-germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4457/15421_A_9_8.jpg
Figure 10 RNA cell line category: Cell line enhanced (HDLM-2, Karpas-707)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000164136-IL15
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000164136-IL15
Figure 10 RNA cell line category: Cell line enhanced (HDLM-2, Karpas-707)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000164136-IL15
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000164136-IL15
Specifications
- Immunogen
- Recombinant human IL15 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human IL15
- Species Reactivity
- Human
- Clone
- R02-5K7
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 0.01M PBS, pH 7.2
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- Interleukin 15
- Gene ID
- 3600
- UniProt ID
- P40933
- Long Name
- Interleukin 15
- Sequence Similarities
- Belongs to the IL-15/IL-21 family.
- Cellular Localization
- Cytoplasm, Nucleus, Secreted
- Protein Refseq
- NP_000576.1
- Function
- Cytokine that plays a major role in the development of inflammatory and protective immune responses to microbial invaders and parasites by modulating immune cells of both the innate and adaptive immune systems. Stimulates the proliferation of natural killer cells, T-cells and B-cells and promotes the secretion of several cytokines.
Recommended Products
Detection antibody
Isotype Control
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Related Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1254z-F(E) | Recombinant Anti-Human IL15 Antibody Fab Fragment | IF, FACS, FuncS | Fab |
| HPAB-0359-WJ-F(E) | Mouse Anti-IL15 Recombinant Antibody (clone IL15-3); Fab Fragment | ELISA | Mouse Fab |
| HPAB-S0072-YC-F(E) | Human Anti-IL15 Recombinant Antibody (clone 4H10); Fab Fragment | ELISA | Human Fab |
| HPAB-S0073-YC-F(E) | Human Anti-IL15 Recombinant Antibody (clone 1H3); Fab Fragment | ELISA | Human Fab |
| HPAB-S0074-YC-F(E) | Human Anti-IL15 Recombinant Antibody (clone 3C1); Fab Fragment | ELISA | Human Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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