Mouse Anti-ITGB3 Recombinant Antibody (clone PT0052) (CAT#: VS3-CJ394)

• Immunogen
• Synthesized peptide derived from human CD61

• Host Species
• Mouse

• Type
• Mouse IgG1, κ

• Specificity
• Human ITGB3

• Species Reactivity
• Human

• Clone
• PT0052

• Applications
• IHC-P

• Conjugate
• Unconjugated

• Purification
• Affinity chromatography

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• 50% Glycerol, 0.5% BSA, PBS.

• Preservative
• 0.02% sodium azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• This antibody has been tested for use in Immunohistochemistry-Parraffin (1:100-1:500).

• Alternative Names
• GP3A; Integrin beta-3; Platelet membrane glycoprotein IIIa; GPIIIa; CD61; GT; CD61; GP3A; BDPLT2; BDPLT16; GT2; BDPLT24

• Gene ID
• 3690

• UniProt ID
• P05106

• Sequence Similarities
• Belongs to the integrin beta chain family.

• Cellular Localization
• Cell junction, Cell membrane, Cell projection, Membrane

• Post Translation Modifications
• Phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Probably involved in outside-in signaling. A peptide (AA 740-762) is capable of binding GRB2 only when both Tyr-773 and Tyr-785 are phosphorylated. Phosphorylation of Thr-779 inhibits SHC binding.

• Protein Refseq
• NP_000203.2

• Function
• Integrin alpha-V/beta-3 (ITGAV:ITGB3) is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 (ITGA2B:ITGB3) is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. Fibrinogen binding enhances SELP expression in activated platelets (By similarity).

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS