Mouse Anti-MAPK8 Recombinant Antibody (clone 1E5)
CAT#: VS7-0425-WR358
This product is a mouse IgG1 antibody that binds to human MAPK8. The antibody 1E5 can be used for WB and FC assays. It cross reacts with mouse.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human breast
Immunohistochemical staining of human breast shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4463/ihc_selected.jpg
Normal Tissue
Figure 2 IHC staining of human thyroid gland
Immunohistochemical staining of human thyroid gland shows moderate cytoplamsic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69396/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4463/12466_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4463/12962_A_7_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4463/12962_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4463/12962_A_4_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4463/12962_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000107643-MAPK8
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Specifications
- Immunogen
- Recombinant human MAPK8 fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human MAPK8
- Species Reactivity
- Human, Mouse
- Clone
- 1E5
- Molecule Class
- Map Kinase
- Applications
- WB, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2
- Gene ID
- 5599
- UniProt ID
- P45983
- Long Name
- Mitogen-Activated Protein Kinase 8
- Cellular Localization
- Cytoplasm; Nucleus; Synapse
- Post Translation Modifications
- Phosphoprotein; S-nitrosylation
- Protein Refseq
- NP_001265476.1
- Function
- Kinase; Serine/threonine-protein kinase; Transferase
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Related Signaling Pathways
FcεR1 Signaling Pathway
TCR Signaling Pathway
Related Diseases
Maturity Onset Diabetes of the Young
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "MAPK8"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1617z | Mouse Anti-MAPK8 Recombinant Antibody (clone 14A7) | WB, ELISA, IHC | Mouse IgG2a, κ |
| MOB-1000CT | Recombinant Mouse anti-Human MAPK8 Monoclonal antibody (4E3) | ELISA, WB | |
| VS3-QX744 | Mouse Anti-MAPK8 Recombinant Antibody (clone 1A4-C5-F11) | WB, IF | Mouse IgG2a |
| VS3-QX745 | Mouse Anti-MAPK8 Recombinant Antibody (clone 1H8-E10-D2) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS13-YC698 | Rabbit Anti-MAPK8 Recombinant Antibody (VS13-YC698) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2159 | Hi-Affi™ Recombinant Rabbit Anti-MAPK8 Monoclonal Antibody (DS2159AB) | WB | IgG |
| MOR-4636 | Hi-Affi™ Recombinant Rabbit Anti-MAPK8 Monoclonal Antibody (TH149DS) | WB, IF, ICC, FC | IgG |
| MOR-4691 | Hi-Affi™ Recombinant Rabbit Anti-MAPK8 Monoclonal Antibody (TH205DS) | WB, IF, ICC, IHC-P, FC, ChIP, ELISA | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC989 | AbPlus™ Anti-MAPK8 Magnetic Beads (VS-0724-YC989) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY292 | Mouse Anti-NHP MAPK8 Recombinant Antibody (clone 4A2G12) | WB, IF | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4244 | Anti-MAPK8 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4245 | Anti-Mouse MAPK8 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4246 | Anti-Rat MAPK8 Immunohistochemistry Kit | IHC |
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