Mouse Anti-MBP Recombinant Antibody (clone PT0183) (CAT#: VS3-CJ302)

• Immunogen
• Synthesized peptide derived from human Myelin Basic Protein (MBP)

• Host Species
• Mouse

• Type
• Mouse IgG1, κ

• Specificity
• Human MBP

• Species Reactivity
• Human

• Clone
• PT0183

• Applications
• IHC-P

• Conjugate
• Unconjugated

• Purification
• Affinity chromatography

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• 50% Glycerol, 0.5% BSA, PBS.

• Preservative
• 0.02% sodium azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• This antibody has been tested for use in Immunohistochemistry-Parraffin (1:100-1:500).

• Alternative Names
• Myelin basic protein; MBP; Myelin A1 protein; Myelin membrane encephalitogenic protein

• Gene ID
• 4155

• UniProt ID
• P02686

• Sequence Similarities
• Belongs to the myelin basic protein family.

• Cellular Localization
• Cell membrane, Membrane, Nucleus

• Post Translation Modifications
• Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
  The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
  Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
  Proteolytically cleaved in B cell lysosomes by cathepsin CTSG which degrades the major immunogenic MBP epitope and prevents the activation of MBP-specific autoreactive T cells.
  Phosphorylated by TAOK2, VRK2, MAPK11, MAPK12, MAPK14 and MINK1.

• Protein Refseq
• NP_001020252.1; NP_001020261.1; NP_001020263.1

• Function
• The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS