Rabbit Anti-MBP Recombinant Antibody (clone R08-9K4)
CAT#: VS3-FY1782
This recombinant primary antibody specific recognizes human MBP, it exhibits cross-reactivity with mouse, rat. The calculated molecular weight of the protein is 33 kDa, while the observed molecular weight of the protein is 18 kDa. It is recommended for western blotting at dilutions of 1/500 to 1/1000.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line HaCaT
Immunofluorescent staining of human cell line HaCaT shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64368/1577_H5_1_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous
nuclear
Neuropil
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2300/6024_B_9_5.jpg
Normal Tissue
Figure 3 Caudate
Glial cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/
membranous
nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2300/6024_B_8_7.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (HL-60, SK-MEL-30)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000197971-MBP
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IHC
Figure 1 Anti-MBP Antibody (VS3-FY1782) in IHC.
Immunohistochemistry staining of paraffin-embedded Brain using the anti-Myelin Basic Protein antibody. High-pressure and temperature Tris-EDTA pH 8.0 was used for antigen retrieval.
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Specifications
- Immunogen
- Synthetic peptide of human myelin basic protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat MBP
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R08-9K4
- Applications
- Western Blot
- MW
- The calculated weight of the target protein is approximately 33 kDa, while the observed molecular weight stands at 18 kDa.
- Related Disease
- Secondary Progressive Multiple Sclerosis and Demyelinating Disease
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Target
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Related Diseases
Asthma
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| Gly-174LC | Recombinant Anti-MBP Antibody (Non-glycosylated) | ELISA | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1267CT | Recombinant Mouse anti-Human MBP Monoclonal antibody (33) | IHC-Fr, IHC-P | |
| ZG-0271C | Mouse Anti-MBP Recombinant Antibody (ZG-0271C) | WB, ELISA | Mouse IgG |
| ZG-0281U | Mouse Anti-MBP Recombinant Antibody (ZG-0281U) | ELISA, WB | Mouse IgG2a |
| VS3-QX757 | Mouse Anti-MBP Recombinant Antibody (clone 408CT17.4.3) | WB, ICC | Mouse IgM |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0807MZ | Chicken Anti-MBP Polyclonal IgY | ICC, IF, IHC, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2184 | Hi-Affi™ Recombinant Rabbit Anti-MBP Monoclonal Antibody (DS2184AB) | IHC-FoFr, IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0216CQ-F(E) | Mouse Anti-MBP Recombinant Antibody (clone 845D3); Fab Fragment | ELISA, WB | Mouse Fab |
| FAMAB-0107-CN-F(E) | Mouse Anti-MBP Recombinant Antibody (clone IN-1); Fab Fragment | WB, IF, IP, Block | Mouse Fab |
| FAMAB-0217-CN-F(E) | Recombinant Human Anti-MBP Antibody Fab Fragment (E2) | WB, ELISA, IP | Human Fab |
| FAMAB-0218-CN-F(E) | Recombinant Human Anti-MBP Antibody Fab Fragment (F11) | WB, ELISA, IP | Human Fab |
| FAMAB-0219-CN-F(E) | Recombinant Human Anti-MBP Antibody Fab Fragment (E6) | WB, ELISA, IP | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0107-CN | Mouse Anti-MBP Recombinant Antibody (clone IN-1) | WB, IF, IP, Block | Mouse IgM, Κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0217-CN-S(P) | Recombinant Human Anti-MBP Antibody scFv Fragment (E2) | WB, ELISA, IP | Human scFv |
| FAMAB-0218-CN-S(P) | Recombinant Human Anti-MBP Antibody scFv Fragment (F11) | WB, ELISA, IP | Human scFv |
| FAMAB-0219-CN-S(P) | Recombinant Human Anti-MBP Antibody scFv Fragment (E6) | WB, ELISA, IP | Human scFv |
| FAMAB-0220-CN-S(P) | Recombinant Human Anti-MBP Antibody scFv Fragment (D1) | WB, ELISA, IP | Human scFv |
| FAMAB-0221-CN-S(P) | Recombinant Human Anti-MBP Antibody scFv Fragment (B5V6) | WB, ELISA, IP | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY316 | Mouse Anti-NHP MBP Recombinant Antibody (clone MBP101) | WB, IF, ELISA | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0125-FY31 | Human Anti-MBP (clone W25MBP10) scFv-Fc Chimera | ADCC, CDC | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC123 | Recombinant Anti-MBP (AA 65-92 x AA 130-156) Biparatopic Antibody, Tandem scFv (Clone E6 x Clone D1) | ELISA, IP, WB | Tandem scFv |
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